上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (5): 544-.

• 论著（基础研究） • 上一篇    下一篇

乳清蛋白水解物诱导的食物口服耐受小鼠血清Th2相关细胞因子的变化

姜 平, 邵 洁   

  1. 上海交通大学 医学院瑞金医院儿内科, 上海 200025
  • 出版日期:2010-05-25 发布日期:2010-05-28
  • 作者简介:姜 平（1981—）, 女, 硕士生；电子信箱: jiangping-2001@163.com。

Changes of serum Th2 related cytokines in lactalbumin hydrolysate induced oral tolerance to food in mice

JIANG Ping, SHAO Jie   

  1. Department of Pediatrics, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-05-25 Published:2010-05-28

PDF (PC)

1349

摘要：

目的 观察乳清蛋白水解物能否诱导口服耐受，阐明Th2相关细胞因子与食物口服耐受之间的关系。方法 乳清蛋白及其水解物喂养BALB/c小鼠（n=176），建立口服耐受模型，实验分为对照A组、乳清蛋白组、部分水解乳清蛋白组、深度水解乳清蛋白组（n=44）；同时，用乳清蛋白致敏、激发BALB/c小鼠（n=83）建立食物过敏模型，实验分为对照B组（n=41）和乳清蛋白致敏组（n=42）。检测各组血清IgE、IgG、IgG1、IL-4、TGFβ1水平；进行小肠病理组织学分析；流式细胞术检测脾CD4+CD25+Foxp3+Treg细胞数量。结果 与对照A组相比，乳清蛋白组血清IgE、IgG1、IgG和IL-4的水平降低，而TGF-β1水平升高, 脾CD4+CD25+Foxp3+Treg细胞百分比升高（P<0.05）；部分水解乳清蛋白组血清IgE和IL-4水平降低，而TGF-β1水平升高，CD4+CD25+Foxp3+Treg细胞百分比增加（P<0.05）；深度水解乳清蛋白组与对照A组各指标的差异无统计学意义（P>0.05）。乳清蛋白致敏组血清IgE和IL-4水平升高，而TGF-β1水平降低，脾CD4+CD25+Foxp3+Treg细胞百分比升高，与对照B组的差异均具有统计学意义(P<0.05)。结论 乳清蛋白和部分水解乳清蛋白可诱导出以Th2型淋巴细胞反应抑制为主要特征的口服耐受，而深度水解乳清蛋白未能诱导出任何有意义的反应。

关键词: 口服耐受, 牛奶过敏, 乳清蛋白, IgE

Abstract:

Objective To observe whether lactalbumin hydrolysate can induce oral tolerance, and explore the relationship between Th2 related cytokines and oral tolerance to food. Methods BALB/c mice (n=176) were fed with lactalbumin and its hydrolysate, the models of oral tolerance were established, and control group A, lactalbumin group, partially hydrolysed lactalbumin group and extensively hydrolysed lactalbumin group (n=44) were divided. Meanwhile, BALB/c mice (n=83) were allergized and stimulated by lactalbumin to establish the models of food allergy, and control group B (n=41) and lactalbumin allergy group (n=42) were divided. The serum levels of IgE, IgG, IgG1, IL-4 and TGF-β1 were detected by ELISA, the pathological features of intestines were analysed, and the percentages of CD4+CD25+Foxp3+Treg in splenic cells were determined by flow cytometry. Results Compared with control group A, the serum levels of IgE, IgG1, IgG and IL-4 in lactalbumin group were lower, while the serum level of TGF-β1 was higher, and the percentage of CD4+CD25+Foxp3+Treg in splenic cells was higher (P<0.05). The serum levels of IgE and IL-4 were lower in partially hydrolysed lactalbumin group, while the serum level of TGF-β1 was higher, and the percentage of CD4+CD25+Foxp3+Treg in splenic cells was higher (P<0.05). There was no significant difference in each parameter between extensively hydrolysed lactalbumin group and control group A (P>0.05). Compared with control group B, the serum levels of IgE and IL-4 in lactalbumin allergy group were higher, while the serum level of TGF-β1 was lower, and the percentage of CD4+CD25+Foxp3+Treg in splenic cell was higher (P<0.05). Conclusion Both lactalbumin and partially hydrolysed lactalbumin may induce oral tolerance with main feature of suppression of Th2 lymphocyte responses, while extensively hydrolysed lactalbumin may induce no significant reaction.

Key words: oral tolerance, milk allergy, lactalbumin, IgE