›› 2010, Vol. 30 ›› Issue (6): 679-.

• 论著(基础研究) • 上一篇    下一篇

磁性纳米介孔材料携载尿激酶的体外溶栓实验研究

杨文芝, 吴 强, 宋蓓雯, 杜新华   

  1. 上海交通大学 第六人民医院眼科, 上海 200233
  • 出版日期:2010-06-25 发布日期:2010-06-28
  • 通讯作者: 吴 强, 电子信箱: wyan559@hotmail.com。
  • 作者简介:杨文芝(1984—), 女, 硕士生;电子信箱: yangwenzhi@sjtu.edu.cn。
  • 基金资助:

    上海交通大学医工(理)交叉研究基金(YG2009ZD203)

In vitro thrombolysis study of magnetic nano-mesoporous materials carrying urokinase

YANG Wen-zhi, |WU Qiang, |SONG Bei-wen, DU Xin-hua   

  1. Department of Ophthalmology, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, |China
  • Online:2010-06-25 Published:2010-06-28
  • Supported by:

    Shanghai Jiaotong University Foundation, YG2009ZD203

摘要:

目的 评价携载尿激酶的磁性纳米介孔材料在体外流体环境中的溶栓效果。方法 制备血栓,接入体外流体模型中,分为4组,分别注入生理盐水、尿激酶、磁性尿激酶和磁性尿激酶加外磁场。称量溶栓前后的血栓质量,计算溶栓率,并记录再通的液体量。结果 磁性尿激酶加外磁场组在靶位置可见明显的磁性尿激酶聚集。尿激酶组和磁性尿激酶组溶栓率相近,分别为(76.89±0.048)%和(75.42±0.094)%(P>0.05),磁性尿激酶加外磁场组溶解率达到(90.45±0.076)%,高于尿激酶组和磁性尿激酶组(P<0.05)。尿激酶组和磁性尿激酶组收集到再通的液体量分别为(42.47±1.834)mL和(39.70±5.028)mL(P>0.05);磁性尿激酶加外磁场组收集到的液体量达到(60.29±3.216)mL,高于前两组(P<0.05)。结论 磁性纳米介孔材料作为溶栓药物的载体可以增强尿激酶溶栓的靶向性,能在流体条件下提高血栓的溶解率,为在体研究静脉阻塞的靶向性溶栓治疗奠定基础。

关键词: 磁性纳米介孔材料, 尿激酶, 体外溶栓

Abstract:

Objective To evaluate the thrombolytic effects of magnetic nano-mesoporous materials carrying urokinase in flow conditions. Methods Thrombosis was prepared and linked to flow model in vitro. Four groups were divided, and were treated with normal saline (blank control group), urokinase (urokinase control group), magnetic urokinase (magnetic urokinase group) and magnetic urokinase plus external magnetic field (magnetic urokinase plus external magnetic field group), respectively. The weight of thrombus before and after thrombolysis was obtained, the rates of thrombolysis were calculated, and the amount of liquid recanalization was recorded. Results Magnetic urokinase aggregation in the target position was clearly observed in magnetic urokinase plus external magnetic field group. There was no significant difference in the rates of thrombolysis between urokinase control group (76.89%±0.048%) and magnetic urokinase group (75.42%±0.094%) (P>0.05), while the rate of thrombolysis in magnetic urokinase plus external magnetic field group (90.45%±0.076%) was significantly higher than those in urokinase control group and magnetic urokinase group (P<0.05). There was no significant difference in the amount of liquid recanalization between urokinase control group [(42.47±1.834) mL)] and magnetic urokinase group [(39.70±5.028) mL] (P>0.05), while the amount of liquid recanalization in magnetic urokinase plus external magnetic field group [(60.29±3.216)mL] was significantly higher than that in urokinase control group and magnetic urokinase group (P<0.05). Conclusion The magnetic nano-mesoporous material as a carrier of thrombolytic drugs can enhance the targeting of urokinase, and improve the rate of thrombosis in flow conditions.

Key words: magnetic nano-mesoporous materials, urokinase, thrombolysis in vitro