上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (11): 1568-.doi: 10.3969/j.issn.1674-8115.2011.11.014

• 论著（基础研究） • 上一篇    下一篇

去势雄性大鼠肾上腺皮质束状带和网状带的变化及低雄激素对COX-2信号通路的影响

施 超, 李艳香, 翟华玲, 姜博仁, 李 影, 夏芳珍, 徐 慧, 乔 洁, 林东平, 陆颖理   

  1. 上海交通大学 医学院附属第九人民医院内分泌科, 上海 200011
  • 出版日期:2011-11-28 发布日期:2011-11-29
  • 通讯作者: 陆颖理, 电子信箱: luy662011@yahoo.com.cn。
  • 作者简介:施 超（1979—）, 男, 住院医师, 硕士生；电子信箱: shichaophy@163.com。
  • 基金资助:

    上海市科委基金（09140903700）

Changes in zona fasciculata and reticularis of adrenal cortex in castrated male rats and effect of low androgen on signal pathway of COX-2

SHI Chao, LI Yan-xiang, ZHAI Hua-ling, JIANG Bo-ren, LI Ying, XIA Fang-zhen, XU Hui, QIAO Jie, LIN Dong-ping, LU Ying-li   

  1. Department of Endocrinology and Metabolism, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-11-28 Published:2011-11-29
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 09140903700

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摘要：

目的 观察去势雄性大鼠肾上腺皮质束状带和网状带的形态结构和功能的变化，探讨低雄激素对环氧化酶2(COX-2)信号通路的影响。方法 30只10周龄雄性SD大鼠随机分为对照组（n=8）、去势组（n=11）和替代组(n=11)，对照组大鼠接受假去势手术，去势组大鼠接受去势手术，替代组大鼠在接受去势手术后给予肌肉注射睾酮（十一酸睾酮每月50 mg/kg）进行替代治疗。10周后采集各组大鼠血清，放射免疫分析法测定血清睾酮（T）、皮质酮（F）、促肾上腺皮质激素（ACTH）、黄体生成素（LH）和促卵泡激素（FSH）水平；取大鼠肾上腺皮质组织，HE染色后光学显微镜观察肾上腺皮质束状带和网状带形态学变化，透射电子显微镜观察超微结构改变；采用RT-PCR技术分别检测大鼠肾上腺皮质和胸腹主动脉组织中COX-2 mRNA表达。结果 与对照组和替代组比较，去势组大鼠血清T水平显著降低（P<0.01），血清F、ACTH、LH和FSH水平显著升高（P<0.05）。去势组大鼠肾上腺皮质束状带和网状带细胞变大，细胞核增大且常染色质增多，线粒体和滑面内质网也较发达，脂滴明显减少；替代组大鼠肾上腺皮质束状带和网状带的细胞形态和超微结构与对照组相似。去势组大鼠肾上腺皮质组织中COX-2 mRNA表达显著低于对照组和替代组（P<0.05），而主动脉血管组织中COX-2 mRNA表达明显高于对照组和替代组（P<0.05）。结论 去势雄性大鼠肾上腺皮质束状带和网状带增生且功能增强，肾上腺皮质组织中COX-2 mRNA表达下调;主动脉血管组织中COX-2 mRNA表达上调，可能与低雄激素导致的血管病变有关。

关键词: 低雄激素, 肾上腺皮质, 环氧化酶2, 血管

Abstract:

Objective To investigate the changes in structure and function of zona fasciculata and reticularis of adrenal cortex in castrated male rats, and explore the effect of low androgen on  the signal pathway of cyclooxgenase-2 (COX-2). Methods Thirty male SD rats aged 10 weeks were randomly divided into control group (n=8), castration group (n=11) and replacement group (n=11). Rats in control group received sham castration, those in castration group underwent castration, and those in replacement group were managed with testosterone undecanoate (50 mg/kg per month) after castration. Ten weeks later, serum samples were collected for determination of serum concentrations of testosterone (T), corticosterone (F), adrenocorticotrophic hormone (ACTH), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by radioimmunoassay, adrenal cortex tissues were obtained for observation of morphological changes in zona fasciculata and reticularis of adrenal cortex with HE staining by light microscopy and ultrastructural changes by transmission electron microscopy, and the expression of COX-2 mRNA in tissues of adrenal cortex and thoracoabdominal aorta was detected by RT-PCR. Results Serum T concentration in castration group was significantly lower than those in control group and replacement group （P<0.01）, and serum concentrations of F, ACTH, LH and FSH in castration group were significantly higher than those in control group and replacement group （P<0.05）. Cells in zona fasciculate and reticularis of adrenal cortex in castration group were larger, with bigger nuclei, more mitochondrion, better-developed smooth endoplasmic reticula and less lipid droplets. However, the morphology and ultrastructure of cells in zona fasciculata and reticularis of adrenal cortex in replacement group were similar to those in control group. The expression of COX-2 mRNA in adrenal cortex tissues in castration group was significantly lower than that in control group and replacement group (P<0.05), while the expression of COX-2 mRNA in aorta tissues in castration group was significantly higher than that in control group and replacement group (P<0.05). Conclusion There are hyperplasia and hyperfunction of zona fasciculate and reticularis of adrenal cortex and lower expression of COX-2 mRNA in adrenal cortex tissues in castrated male rats, and the increased expression of COX-2 mRNA in aorta tissues may be related to the vascular disease induced by low androgen.

Key words: low androgen, adrenal cortex, cyclooxgenase-2, blood vessel