JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (3): 314-319.doi: 10.3969/j.issn.1674-8115.2021.03.005

• Basic research • Previous Articles     Next Articles

Expression patterns of interferon regulatory factor 2 binding protein 2 during the early embryonic development of zebrafish

Hai-hong WANG(), Shuo GAO, Jun ZHU(), Jun ZHOU()   

  1. Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Received:2020-04-01 Online:2021-03-28 Published:2021-04-06
  • Contact: Jun ZHU,Jun ZHOU E-mail:wanghaihong51@163.com;zhuj1966@yahoo.com;junjun_j@yahoo.com

Abstract: Objective

·To explore the expression similarities and differences of the two paralogs of interferon regulatory factor 2 binding protein 2 (irf2bp2) gene (irf2bp2a and irf2bp2b) during the early embryonic development of zebrafish.

Methods

·Zebrafish embyos at different developmental stages (embryonic and larval stages) were chosen as research object. The mRNA whole mount in situ hybridization was used to detect the temporal and spatial expression patterns of irf2bp2a and irf2bp2b, and real-time quantitative PCR (qPCR) was used to detect their expression levels.

Results

·Although irf2bp2a and irf2bp2b shared similar expression patterns in the nervous system, eye and pectoral fin, irf2bp2a and irf2bp2b were specifically expressed in liver and stannius corpuscles, respectively, suggesting their expressions were quite unique in certain organs. The results of qPCR showed that both irf2bp2a and irf2bp2b were expressed in the early embryonic development stage, but their expression levels were different.

Conclusion

·Although the functional domains of irf2bp2a and irf2bp2b are highly conserved, the two paralogs of zebrafish irf2bp2 display some distinctions in the expression patterns during the early embryonic development of zebrafish, which suggests that their functions might not be redundant.

Key words: interferon regulatory factor 2 binding protein 2 (irf2bp2), zebrafish embyo, expression pattern, mRNA whole mount in situ hybridization, real-time quantitative PCR (qPCR)

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