›› 2011, Vol. 31 ›› Issue (5): 580-.doi: 10.3969/j.issn.1674-8115.2011.05.012

• Original article (Basic research) • Previous Articles     Next Articles

Effects of serum of patients with acute motor axonal neuropathy on expression of apoptosis-related proteins in rat spinal motor neuron

PENG Li-wei1, YANG Guo-feng1, JI Jian-guo2, WEI Xiao-shan1, LI Pei1, ZHAO Jing-ru1, ZHANG Rui-li1, FENG Xiao1   

  1. 1.Department of Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China；2.National Laboratory of Protein Engineering, School of Life Science, Peking University, Beijing 100871, China
  • Online:2011-05-28 Published:2011-05-27
  • Supported by:

    National Natural Science Foundation of China, 30500164；Natural Science Foundation of Hebei Province, C2007000848

PDF (PC)

1958

Abstract:

Objective To investigate the effects of serum of patients with acute motor axonal neuropathy(AMAN) on expression of apoptosis-related proteins in rat spinal motor neuron with proteomics. Methods Serum samples of 16 patients with AMAN and 16 healthy people were obtained, and two-dimensional gel electrophoresis was performed 24 h after primary culture of rat spinal motor neuron. The proteins of interest were identified by MALDI-TOF/TOF tandem mass spectrometry, and differential protein spots were defined as those with changes of expression >30%. Results There were 21 differentially expressed proteins, among which heterogeneous nuclear ribonucleoprotein H, proteasome subunit alpha type 1, endoplasmic reticulum protein 29, glyceraldehyde-3-phosphate dehydrogenase and voltagedependent anion-selective channel protein 1 were apoptosis-related proteins. Conclusion Serum of patients with AMAN may lead to changes of expression of apoptosis-related proteins in rat spinal motor neuron.

Key words: acute motor axonal neuropathy, proteomics, serum, apoptosis-related protein