›› 2011, Vol. 31 ›› Issue (6): 733-.doi: 10.3969/j.issn.1674-8115.2011.06.012

• Original article (Basic research) • Previous Articles     Next Articles

Evaluation of transfection efficiency of novel non-viral nano gene vector PEI-β-CyD on chondrocytes and bone marrowderived mesenchymal stem cells

TONG Hai-jun1, HUANG Jin-gang1, TANG Gu-ping2, DAI Ke-rong1,3, ZHANG Xiao-ling1,3   

  1. 1.Laboratory of Orthopedical Cellular and Molecular Biology, Institute of Health Sciences, Shanghai Jiaotong University School of Medicine &|Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, China；2.Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China；3.Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedics, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-06-28 Published:2011-06-27
  • Supported by:

    Foundation of the Ministry of Science and Technology of China, S2011GR0323；National Natural Science Foundation of China, 30811120440, 30871435；Foundation of Shanghai Science and Technology Committee, 09540703600；Foundation of Shanghai Education Committee, J50206

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Abstract:

Objective To evaluate the transfection efficiency of novel non-viral nano gene vector polyethyleneimine-β-cyclodextrin (PEI-β-CyD) on chondrocyte cell line C5.18 and bone marrow-derived mesenchymal stem cell line C3H10T1/2. Methods Chondrocyte cell line C5.18 and bone marrow-derived mesenchymal stem cell line C3H10T1/2 were cultured in vitro, and the cytotoxicity of PEI-β-CyD and PEI25KDa (polyethyleneimine of 25 000 in molecular weight) on C5.18 cells and C3H10T1/2 cells was observed and compared by MTT method. C5.18 cells and C3H10T1/2 cells were transfected with PEI-β-CyD and PEI25KDa respectively under different ratios of effective nitrogen content in vectors to effective phosphorus content in exogenous gene (N/P), and the transfection efficiency was analysed by inverted fluorescent microscopy and flow cytometry. Results The cytotoxicity of PEI-β-CyD was significantly lower than that of PEI25KDa to both C5.18 cells and C3H10T1/2 cells. For C5.18 cells, there was no significant difference in transfection efficiency between PEI-β-CyD and PEI25KDa (P>0.05), and for C3H10T1/2 cells, transfection efficiency of PEI-β-CyD was significantly higher than that of PEI25KDa (P<0.05). Conclusion PEI-β-CyD is an ideal non-viral nano gene vector with low cytotoxicity and favorable transfection efficiency for chondrocyte cell line C5.18 and bone marrow-derived mesenchymal stem cell line C3H10T1/2, which is promising in the research and application of cartilage tissue engineering and cell therapy.

Key words: non-viral nano gene vector, PEI-β-CyD, cytotoxicity, transfection efficiency