›› 2011, Vol. 31 ›› Issue (11): 1564-.doi: 10.3969/j.issn.1674-8115.2011.11.013

• Original article (Basic research) • Previous Articles     Next Articles

Effects of co-treatment with puerariae radix flavones and arsenic trioxide on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells

TANG Yu-hong1, SHAO Hua-min2, ZHU Hong-qing3, JIANG Peng-jun4, JI Jian-min4, SHEN Qun4   

  1. 1.Department of Hematology, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China；2.Department of Hematology and Oncology, No.155 Hospital of People's Liberation Army, Kaifeng 475003, China；3.Department of Hematology, The Central Hospital of Maanshan, Maanshan 243000, China；4.Department of Hematology, the Hospital Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210009, China
  • Online:2011-11-28 Published:2011-11-29
  • Supported by:

    Foundation of Department of Health, Jiangsu Province, RC2007068；Talent Program of Jiangsu Province, 2008

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Abstract:

Objective To investigate the effects of co-treatment with puerariae radix flavones (PRF) and arsenic trioxide (ATO) on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells of acute myeloid leukemia M2. Methods Kasumi-1 cells and HL-60 cells were treated with 100 μg/mL PRF and 1 μmol/L ATO for 48 h (RRF+ATO group). MTT assay was employed to measure the inhibition rate of cell proliferation, Annexin V-FITC/PI double staining was used to determine the early apoptosis rate by flow cytometry, the expression of Bcl-2 and Survivin mRNA was detected by Real-Time PCR, and the expression of Caspase-3, Caspase-8 and Caspase-9 protein was detected by Western blotting. Cells only treated with 100 μg/mL PRF were served as PRF group, and blank control group was also established. Results The inhibition rate of cell proliferation, early apoptosis rate and expression of Caspase-3 and Caspase-9 protein of Kasumi-1 cells in RRF+ATO group were significantly higher than those in PRF group (P<0.05), and there was an obvious trend of down-regulation of expression of Bcl-2 mRNA in Kasumi-1 cells. There was no significant difference in each parameter of HL-60 cells between RRF+ATO group and PRF group (P>0.05). Conclusion Co-treatment with PRF and ATO can effectively inhibit the proliferation and induce early apoptosis of Kasumi-1 cells, while that has no significant effect on HL-60 cells.

Key words: puerariae radix flavones, arsenic trioxide, proliferation, apoptosis, acute myeloid leukemia M2