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Treatment of female primary bladder neck obstruction with transurethral resection of bladder neck and pathological analysis ZHANG Xiao-peng, HU Hao, ZHANG Guo-xi, et al    2012, 32 (4): 408-.   DOI: 10.3969/j.issn.1674-8115.2012.04.008 Abstract （7899）      PDF（pc） （294KB）（1225）       Knowledge map    Save Objective To investigate the outcomes of female primary bladder neck obstruction (PBNO) treated with transurethral resection of bladder neck (TURBN), and perform the pathological analysis. Methods Twenty-two women with PBNO were recruited, and preoperative examinations were conducted, including physical examination, urine routine, American Urological Association scoring, urodymamics, ultrasonography and cystoscopy. Bladder outlet obstruction was diagnosed according to Blaivas-Groutz nomogram, TURBN was performed with Olympus standard resectoscope, and urinary catheter was retained for 24 to 72 h after operation. Results There were 4 patients with severe obstruction, 10 patients with moderate obstruction and 8 patients with mild obstruction. Elevated posterior lip of bladder neck and trabecula hyperplasia of bladder wall were observed through cystoscopy. Twelve months after operation, all the symptoms and parameters were improved. Pathological examinations revealed glandular cystitis, fibrous tissue hyperplasia and chronic inflammation. Conclusion TURBN is an effective and safe method in treatment of PBNO, especially for patients with unsuccessful pharmacotherapy. Related Articles | Metrics

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Primary arterial switch operation for D-transposition of the great arteries with intact ventricular septum in children older than 3 weeks XIE Feng, CHEN Hui-wen, LIU Jin-fen    2011, 31 (9): 1250-.   DOI: 10.3969/j.issn.1674-8115.2011.09.008 Abstract （7527）      PDF（pc） （4379KB）（1416）       Knowledge map    Save Objective To assess the outcomes of primary arterial switch operation (ASO) in treatment of D-transposition of the great arteries (DTGA) with intact ventricular septum (IVS) in children older than 3 weeks, and explore the significance of helix ventricular myocardial band (HVMB) in the treatment of DTGA/IVS. Methods The clinical data of 54 children with DTGA/IVS aged between 3 weeks and 16 months (late ASO group) and 74 children with DTGA/IVS aged less than 3 weeks (early ASO group) were analysed. In late ASO group, children were subdivided into ventricular septal migration (VSM) group (n=20, ventricular septa were straight or skewed to the left) and ventricular septal normal (VSN) group (n=34, ventricular septa were normal in appearance). The surgical outcomes of ASO were evaluated. Results There was no significant difference in time of extracorporeal circulation, time of aortic clamping, time of ventilator application after operation, time of intensive care after operation, duration of hospitalization and mortality between late ASO group and early ASO group (P＞0.05). There was no significant difference in mortality between VSM group and VSN group (10.0% vs 8.8%, P＞0.05). Conclusion Primary ASO may be appropriate treatment for children with DTGA/IVS aged between 3 weeks and 16 months, and the normal appearance of ventricular septa plays an important role in the outcomes, which can be well explained by the theory of HVMB. Related Articles | Metrics

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Project introductionShanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support

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Application of Cytokeratin 19, HBME-1 and Galectin-3 in diagnosis of thyroid papillary carcinoma DU Guang-ye, LI Xiao-qiang, LIU Li-wei, et al    2010, 30 (4): 448-.   Abstract （5662）      PDF（pc） （5702KB）（1595）       Knowledge map    Save Objective To investigate the value of Cytokeratin 19 (CK19), HBME-1 and Galectin-3 in differential diagnosis of papillary carcinoma of thyroid and thyroid disease with papillary hyperplasia. Methods The specimens of 289 cases of thyroid lesions, including 144 cases of classic papillary carcinoma (CPC), 21 cases of follicular variant papillary carcinoma (FVPC), 19 cases of papillary microcarcinoma (PM), 46 cases of nodular goiter with papillary hyperplasia (NGWPH) and 59 cases of follicular adenoma with papillary hyperplasia (FAWPH) were collected. The expression of CK19, HBME-1 and Galectin-3 was detected by EnVision system. Results CK19, HBME-1 and Galectin-3 expressed in 100%, 93.1% and 89.6% of CPC, in 100%, 76.2% and 76.2% of FVPC, in 100%, 73.7% and 78.9% of PM, in 5.1%, 2.2% and 4.3% of NGWPH, and in 4.3%, 1.7% and 5.1% of FAWPH, respectively. There were significant differences between thyroid papillary carcinoma and thyroid diseases with papillary hyperplasia in expression of CK19, HBME-1 and Galectin-3 (P<0.01). Conclusion CK19 is a sensitive marker for thyroid papillary carcinoma, and HBME-1 and Galectin-3 are better in specificity. This panel of markers is useful to differentiate papillary carcinoma of thyroid from thyroid papillary hyperplasia. Related Articles | Metrics

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Comparison of references for assessment of overweight and obesity in children of Shanghai CHEN Lei, TANG Qing-ya, RUAN Hui-juan, et al    2010, 30 (1): 36-.   Abstract （5649）      PDF（pc） （3179KB）（1330）       Knowledge map    Save Objective To compare and analyse four references for assessment of obesity in Chinese children. Methods The height and weight of 2 347 children (1 175  boys and 1 172 girls) aged 7 to 8 years in Shanghai were measured, and their body mass indexes (BMI) were calculated. The prevalences of overweight and obesity were evaluated and compared among reference of Weight for Height 1985 (WFH 1985 reference), BMI reference of Working Group on Obesity in China (WGOC reference), BMI reference of Europe International Obesity Task Force (IOTF reference) and BMI reference of Centers for Disease Control and Prevention of American 2000 (CDC reference). Results The prevalence of overweight in boys evaluated by IOTF reference was significantly higher than those by the other three references (P<0.05), and the prevalence of overweight in girls evaluated by IOTF reference was significantly higher than those by WGOC and CDC references (P<0.05). The prevalence of obesity in boys evaluated by IOTF reference was significantly lower than those by the other three references (P<0.01), and the prevalence of obesity in girls evaluated by IOTF reference was significantly lower than those by WGOC and WFH 1985 references (P<0.01). There was no significant difference in the evaluation findings of obesity and overweight between WFH 1985 and WGOC references (P>0.05). Conclusion WFH 1985 and WGOC references are more suitable than IOTF and CDC references for assessment of overweight and obesity in Chinese children. Related Articles | Metrics

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Isolation, identification and growth characteristics of Porphyromonas gingivalis clinical strains LI Yun-peng, LIU Da-li, SHU Rong, et al    2012, 32 (1): 64-.   DOI: 10.3969/j.issn.1674-8115.2012.01.012 Abstract （5215）      PDF（pc） （6147KB）（2618）       Knowledge map    Save Objective To isolate and identify Porphyromonas gingivalis (P. gingivalis) from clinical subjects, and analyse the growth characteristics of P. gingivalis. Methods Subgingival plaque samples were collected from patients with chronic periodontitis and periodontally healthy subjects, and anaerobic culture was performed. P. gingivalis strains were identified by PCR and 16S rDNA sequencing, and detection rate was analysed. Bacteria colonies and growth characteristics of the clinical strains of P. gingivalis were observed, and the biological properties were analysed. Results Six P. gingivalis clinical strains named as L2, L3, L4, L5, L11 and L12 were isolated from 146 subgingival plaque samples collected from 35 subjects. The detection rate of P. gingivalis was 27.4% in subgingival plaque samples of periodontally healthy subjects, 86.7% in periodontally healthy sites of patients with chronic periodontitis, 95.6% in periodontal pockets with probing depth of 4-6 mm, and 96.0% in periodontal pockets with probing depth >6 mm. Strains L2, L5, L11 and L12 formed black colonies on BHI plates after culture for 1 week. Strains L3 and L4 formed grey colonies 1 week after culture for 1 week, and formed black colonies after culture for 10 d. Strains L2, L4, L5 and L12 grew to exponential phase 30 h after inoculation, whereas strains L3 and L11 grew to exponential phase 60 h after inoculation. Conclusion P. gingivalis is one of the dominant pathogenic bacteria in periodontal pockets of patients with chronic periodontitis. The detection rate of P. gingivalis in healthy sites of patients with chronic periodontitis is significantly higher than that of periodontally healthy subjects, and the detection rates of P. gingivalis are higher in deeper periodontal pockets. The growth characteristics of P. gingivalis clinical strains vary from each other, indicating that the biological and pathogenic properties of P. gingivalis may be strain-specific. Related Articles | Metrics

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Influence of depth of burn wound on volume of fluid infusion during shock stage CHEN Zheng-li, YUAN Ke-jian    2011, 31 (1): 64-.   DOI: 10.3969/j.issn.1674-8115.2011.01.015 Abstract （5107）      PDF（pc） （4240KB）（1461）       Knowledge map    Save Objective To explore the relationship between depth of burn wound and volume of fluid infusion during shock stage. Methods The clinical data of patients with total burn surface area no less than 25% of total body surface area and undergoing fluid infusion by Ruijin resuscitation formula during shock stage were collected. The total burn surface area, full-thickness burn surface area and volumes of fluid infusion in the first and second 24 h after burn were recorded, the ratio of full-thickness burn surface area to burn surface area (full-thickness burn surface area ratio) was calculated, and volumes infused per body weight and burn surface area （VIWB）in the first and second 24 h after burn were obtained. The relationship between full-thickness burn surface area ratio and VIWB was explored, the regression equations were established, and the corrected VIWB in the first and second 24 h after burn were calculated. Results The data of 166 patients were included. Compared with VIWB of patients with superficial burn （n=53）, those of patients with fullthickness burn （n=7） increased 37% and 41% in the first and second 24 h after burn, respectively (P<0.05). Fullthickness burn surface area ratio was significantly positively related to VIWB in the first and second 24 h after burn (R2=0.138, P<0.001; R2=0.108, P<0.001). The regression equations were y=0.537x+1.595 and y=0.314x+0.775. Compared with corrected VIWB in the first and second 24 h after burn of patients with superficial burn, those of patients with full-thickness burn and same burn surface area increased 35% and 41%, respectively. With each 20% increase in full-thickness burn surface area ratio, the corrected VIWB in the first and second 24 h after burn increased 0.1 and 0.06, respectively. Conclusion Full-thickness burn surface area ratio is related to volume of fluid infusion during shock stage, and corrected formula is suitable to VIWB of different depth of burn wound. Related Articles | Metrics

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Value of brain natriuretic peptide and N-terminal pro-brain natriuretic peptide in diagnosis of heart failure in the elderly CAI Qin, JIN Yu-hua, FANG Ning-yuan    2009, 29 (10): 1226-.   Abstract （5011）      PDF（pc） （4225KB）（1676）       Knowledge map    Save Objective To investigate the relationship between brain natriuretic peptide(BNP)/N-terminal pro-brain natriuretic peptide (NT-proBNP) and New York Heart Association (NYHA) classification, left ventricular structure and function, and explore the value of BNP/NT-proBNP in the diagnosis of heart failure in the elderly. Methods Fifty-five elder patients with heart failure were selected (NYHA Ⅱ, n=15; NYHA Ⅲ, n=25; NYHA IV, n=15) (heart failure group), and another 16 elder people with NYHA I were served as control group.The plasma mass concentrations of NT-proBNP and BNP were detected by electrochemiluminescence immunoassay and immunofluorescence method, respectively, and the structure and function of left ventricle were examined by echocardiography. Results The levels of plasma BNP and NT-proBNP increased with NYHA grades, were negatively correlated with LVEF (P<0.001), and were positively correlated with LVST, LVEDD, LAD and LVMI(P<0.05). The area under the curve of BNP in diagnosis of heat failure was 0.879 (P<0.001), and that of NT-proBNP was 0.914（P<0.001）. Conclusion Both plasma BNP and NT-proBNP can be used to evaluate the heart function of patients with heart failure, and are useful tools for diagnosis of HF in the elderly. Related Articles | Metrics

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GPER: a novel target in treatment of estrogen-related diseases WANG Jie, Haji Akber AISA, MA Hai-rong, et al    2013, 33 (2): 225-.   DOI: 10.3969/j.issn.1674-8115.2013.02.020 Abstract （4858）      PDF（pc） （492KB）（1125）       Knowledge map    Save G protein-coupled estrogen receptor 1 (GPER) is a novel receptor, which belongs to the family of G protein-coupled receptors, to many of the rapid biological responses to estrogen. Different from the two classical nuclear estrogen receptors, ERα and ERβ, GPER modulates both rapid non-genomic reaction and genomic transcriptional events of estrogen. In this regard, GPER may be considered as a valuable target toward novel therapeutic strategy for many estrogen-related diseases. The intrinsic appearance of GPER, including structure, subcellular localization, ligands, signal transduction pathway and relationship with diseases is reviewed in this paper. Related Articles | Metrics

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Ubiquitin-like modification (SUMOylation) and genomic stability CHENG Jin-ke    2018, 38 (7): 719-.   DOI: 10.3969/j.issn.1674-8115.2018.07.001 Abstract （4844）      PDF（pc） （3290KB）（1387）       Knowledge map    Save The mechanism of protein post-translational modifications in regulation of cell mitosis and genomic stability is always a hot issue in biological researches. The present review introduced the functional activity of de-SUMOylase SENP3 in sister chromatin dissociation at mitosis, and demonstrated a new molecular mechanism of cross-talk regulation between protein SUMOylation and phosphorylation in maintaining genomic stability. Related Articles | Metrics

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Clinical significance of food allergen-specific IgG detection CHEN Yi, CHEN Tong-xin, ZHU Ya-zhong    2010, 30 (2): 143-.   Abstract （4717）      PDF（pc） （3798KB）（1666）       Knowledge map    Save Objective To explore the clinical significance of detection of 14 kinds of food allergen-specific IgG. Methods Fourteen kinds of food allergen-specific IgG were detected by ELISA method in 211 patients with allergic diseases, and IgG positive rates of various foods were compared among patients with different sex, age and allergic diseases. Results Positive food allergen-specific IgG was detected in 193 (91.4%) patients. Among 14 kinds of foods, the positive rate of food allergen-specific IgG was the highest for eggs (73.9％), and milk came the second. However, no elevated food allergen-specific IgG was observed for chicken and meat. Milk was the most common sensitizers for 0-12 month-old patients, and egg was the first cause for the other age groups. There were significant differences in the positive rates of food allergen-specific IgG for milk among different age groups (P<0.01). There was no significant difference in the positive rate of food allergen-specific IgG between males and females (P>0.05). The positive rate of food allergen-specific IgG in patients with eczema was the highest (96.4%), and the lowest (83.3%) was found in those with chronic diarrhea, while there was no significant difference among different diseases (P>0.05). The positive rate of food allergen-specific IgG for milk differed significantly among different diseases (P<0.01). Positive food allergen-specific IgG was detected in 12 kinds of food (except for chicken and meat) for patients with allergic purpura. Conclusion Food intolerance is a complex allergy. The food allergen-specific IgG detection is of great importance as reference for etiologic diagnosis of allergic diseases. Related Articles | Metrics

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Feasibility on treating indigestible cell lines by trypsin-EDTA solution for detection of cell apoptosis FU Rong, ZHAO Ben-peng, YANG Jie, et al    DOI: 10.3969/j.issn.1674-8115.2015.09.036

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Expression of CypB in colorectal cancer tissues and its effects on |metastasis and invasion of cancer cells YUE Fei, ZHENG Min-hua, LU Ai-guo, et al    2010, 30 (3): 288-.   Abstract （4266）      PDF（pc） （4317KB）（1614）       Knowledge map    Save Objective To investigate the expression of cyclophilin B (CypB) in colorectal cancer tissues with or without lymph node metastasis, and explore the effects of CypB plasmids knockdown on metastasis and invasion of cancer cells. Methods The expression of CypB in 59 colorectal cancer tissue microarray, including 31 without lymph node metastasis and 28 with lymph node metastasis, were evaluated with immunohistochemistry. Colorectal cancer cell line SW1116 was transfected with shRNA plasmids targeting CypB(SW1116-siCypB) and negative control plasmids(SW1116-NC). CypB knockdown effects were analysed with Western blotting. Wound healing assay and transwell cell invasion assay were performed to observe the effects of CypB knockdown on metastasis and invasion of SW1116 cells. Results The expression of CypB in colorectal cancer tissues with lymph node metastasis was significantly higher than that in colorectal cancer tissues without lymph node metastasis (P<0.01). Compared with SW1116-NC, the expression of CypB of SW1116-siCypB significantly reduced (P<0.01), and the performance of metastasis and invasion significantly decreased (P<0.01). Conclusion CypB plays an important role in lymph node metastasis of colorectal cancer, which makes it a potential target for the prevention and treatment of lymph node metastasis of colorectal cancer. Related Articles | Metrics

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Comparison analysis of researches on mental health of university students in China and abroad LU Wei, ZHAO Min    2012, 32 (10): 1382-.   DOI: 10.3969/j.issn.1674-8115.2012.10.023 Abstract （4262）      PDF（pc） （404KB）（2517）       Knowledge map    Save The research on mental health of university students has been developing rapidly in China in recent years, but it still significantly lags behind that of the developed countries. Through analysis of related domestic and overseas literatures, the status of mental health and research method of mental health of university students in China are summarized, and are compared with those of the other countries in this paper. It is suggested that the team building, methodology, horizon and range of research on mental health of university students should be strengthened in China. Related Articles | Metrics

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Culture and differentiation of C2C12 cells for identification of skeletal muscular fibers ZHOU Li-na, WANG Yan, LI Qing-yun, et al    DOI: 10.3969/j.issn.1674-8115.2013.10.024

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2011, 31 (9): 1218-.   Abstract （3657）      PDF（pc） （2895KB）（936）       Knowledge map    Save Related Articles | Metrics

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Impact of bilateral ovariectomy on climacteric symptoms in post-menopausal women TAO Min-fang, ZHU Jie-ping, TENG Yin-cheng    2010, 30 (2): 162-.   Abstract （3570）      PDF（pc） （3123KB）（1581）       Knowledge map    Save Objective To evaluate the impact of bilateral ovariectomy in post-menopausal women by comparison of climacteric symptoms before and after operation. Methods Post-menopausal women who received hysterectomy with concurrent bilateral ovariectomy on benign uterine indications were recruited. Climacteric symptoms before and after operation were accessed by a questionaire including modified Kupperman Index. Comparison was made after grouping the patients into early menopause group, whose menopausal period was no more than 5 years, and late menopause group, whose menopausal period was more than 5 years. Results Among a total of 95 individuals, 8 had normal, 46 had mild, 34 had moderate, and the other 7 had severe Kupperman Index scores before operation, while 5 had normal, 28 had mild, 48 had moderate, and the other 14 had severe Kupperman Index scores after operation, with more moderate and severe climacteric symptoms after operation than before operation (P<0.05). Women of early menopause group had higher total Kupperman Index scores and symptom scores of sweating, insomnia, depression, fatigue and headache than those of late menopause group before operation (P<0.05). Women of early menopause group had significantly higher Kupperman Index scores after operation than before operation (23.93±7.53 vs 17.63±8.07, P<0.05). However, there was no significant difference in total Kupperman Index scores in women of late menopause group before and after operation. Conclusion Bilateral ovariectomy may affect the climactic symptoms in post-menopausal women, especially in early menopausal period. Related Articles | Metrics

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Comparison of effects of cyclopentolate hydrochloride, compound tropicamide and atropine on cycloplegia DONG Ling-yan, KANG Xiao-li, WANG Ya-fu    2011, 31 (10): 1432-.   DOI: 10.3969/j.issn.1674-8115.2011.10.016 Abstract （3523）      PDF（pc） （4153KB）（1767）       Knowledge map    Save Objective To compare the effects of cyclopentolate hydrochloride, compound tropicamide and atropine on cycloplegia in children. Methods Forty children (80 eyes) with refractive errors, aged from 6 to 12, were randomly divided into 2 groups. Children in group A were subjected to cycloplegic refraction by compound tropicamide, followed with cyclopentolate hydrochloride 2 weeks later. Children in group B were administered cyclopentolate hydrochloride, followed with atropine 2 weeks later. Subjective and objective refraction, pupil size, residual accommodation and systemic side effects were observed. Results After application of two agents in group A, there were significant differences in residual accommodation between two agents, while there was no significant difference in pupil size between two agents, with 82.5%, 85.0% and 85.0% in agreement with the degree of spherical lens, cylinder lens and axis of astigmatism respectively. In group B, there was no significant difference in pupil size and residual accommodation between two agents, with 90.0%, 92.5% and 92.5% in agreement with the degree of spherical lens, cylinder lens and axis of astigmatism respectively. Conclusion Cyclopentolate hydrochloride is a safe and effective cycloplegic, which can be widely used in cycloplegic refraction for children. Related Articles | Metrics

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Progression of renal tubulointerstitial fibrosis and expression of α-SMA, TGF-β 1 and VDR in rat UUO models XIE Sheng-bin, WANG Wei-ming, CHEN nan    2010, 30 (7): 752-.   Abstract （3517）      PDF（pc） （12513KB）（1729）       Knowledge map    Save Objective To observe the progression of renal tubulointerstitial fibrosis in rat unilateral ureteral obstruction (UUO) models, and investigate the changes in expression of renal fibrosis-related proteins during the pathological course. Methods Thirty-two SD rats were randomly divided into sham-operation group (n=16) and UUO model group (model group, n=16). Rats were sacrificed 2, 5, 9 and 14 d after operation (after model establishment for model group) (n=4 for each day), and the renal tissues of the operated side (obstructed side for model group) were obtained. HE staining, Masson staining, immunohistochemical staining and Western blotting were used to evaluate the degree of renal tubulointerstitial fibrosis and detect the expression of α-smooth muscle actin (α-SMA), fibronectin (FN), vitamin D receptor (VDR) and transforming growth factor-β1 (TGF-β1) in renal tissues. Results Histological observations of renal tissues revealed that the degree of renal tubulointerstitial fibrosis gradually increased with time of ureteral obstruction. It was indicated by immunohistochemical staining that the percentages of area with positive α-SMA and FN in renal interstitium of model group, which gradually increased with time of ureteral obstruction and reached the peak 14 d after model establishment, were significantly higher than those of sham-operation group at each time point after model establishment (P<0.05). Western blotting analysis demonstrated that the relative expression of α-SMA and TGF-β1 at each time point after model establishment in model group was significantly higher than that in sham-operation group (P<0.05), while the relative expression of VDR was significantly lower than that in sham-operation group (P<0.05). The relative expression of α-SMA in renal tissues of rats in model group significantly increased on the second day after model establishment, and the relative expression of α-SMA and TGF-β1 in model group was 12.7 times and 8.8 times respectively of that in sham-operation group on the fourteenth day after model establishment, while the relative expression of VDR decreased to 3% of sham-operation group. Conclusion Significant interstitial fibrosis may emerge at 14 d after model establishment in rat UUO models.The expression of α-SMA in renal interstitium increases in the early stage of model establishment, indicating the activation of renal interstitial fibroblasts. The progression of decrease in expression of VDR indicates VDR may relate to renal interstitial fibrosis. Related Articles | Metrics

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Regulation effects of Nocodazole with different concentrations on Hela cell synchronization in G 2/M phase XU Qiu-fang, YU Ke-hua, YI Ting, et al    2010, 30 (3): 284-.   Abstract （3500）      PDF（pc） （5035KB）（2224）       Knowledge map    Save Objective To investigate the regulation effects of Nocodazole with different concentrations on Hela cell synchronization in G2/M phase and spindle morphology. Methods Hela cells were treated with 3.0, 1.0 and 0.3 μmol/L Nocodazole, respectively for 18 h (Nocodazole 3.0, 1.0, 0.3 μmol/L group, respectively). Cells were harvested 0 h, 3 h, 6 h and 9 h after removal of Nocodazole, and the percentage of cells in G2/M phase was detected by flow cytometry. Besides, the arrangement of α-tubulin of cells was observed by confocal microscopy with indirect immunofluorescence staining. Hela cells without treatment with Nocodazole were served as controls. Results After treatment with Nocodazole for 18 h, the percentages of Hela cells in G2/M phase in Nocodazole 3.0, 1.0, 0.3 μmol/L group were 55.95%, 51.09% and 47.81%, respectively, and were significantly higher than that in control group (9.54%)（P<0.05）. There was no significant change in the percentages of Hela cells in G2/M phase in Nocodazole 3.0 and 1.0 μmol/L group 3, 6 and 9 h after removal of Nocodazole, while that in Nocodazole 0.3 μmol/L group decreased significantly （30.43%, 12.91% and 10.23%）, and there had been no significant difference in the percentage of Hela cells in G2/M phase with control group 6 h after removal of Nocodazole （P>0.05）. After removal of Nocodazole, microtubules of cells in G2/M phase in Nocodazole 0.3 μmol/L group reaggregated, and two-pole spindles were formed, with clear spindle fibers. Conclusion Nocodazole can regulate Hela cell synchronization in G2/M phase. After removal of Nocodazole, those treated with 0.3 μmol/L Nocodazole may recover better in cell cycle and spindle morphology. Related Articles | Metrics