上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (4): 400-.doi: 10.3969/j.issn.1674-8115.2013.04.004

• 论著（基础研究） • 上一篇    下一篇

肥胖的非糖尿病大鼠对急性炎症刺激的耐受性研究

章 瑶, 韩亭亭, 苏布德格日乐, 姜冬冬, 李阳雪, 胡耀敏   

  1. 上海交通大学 医学院附属仁济医院内分泌代谢病科, 上海 200127
  • 出版日期:2013-04-28 发布日期:2013-05-03
  • 通讯作者: 胡耀敏, 电子信箱: amin99@163.com。
  • 作者简介:章 瑶 (1989—), 女, 硕士生； 电子信箱: 475511243@qq.com。
  • 基金资助:

    国家自然科学基金（81170758, 30670988, 81270946）

Responsive ability of obese non-diabetic rats to acute inflammatory stimulus

ZHANG Yao, HAN Ting-ting, SU Budegerile, JIANG Dong-dong, LI Yang-xue, HU Yao-min   

  1. Department of Endocrinology and Metabolic Disease, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2013-04-28 Published:2013-05-03
  • Supported by:

    National Natural Science Foundation of China, 81170758, 30670988, 81270946

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摘要：

目的 比较由高脂膳食诱发肥胖的非糖尿病和2型糖尿病大鼠对急性炎症刺激的反应能力。方法 对2型糖尿病GK大鼠（GK组）和Wistar大鼠（Wistar组）进行高脂饲料喂养14周，观察两组大鼠体质量的变化情况。按随机数字表法将高脂饲料喂养14周后的大鼠再分为GK高脂喂养基线组（GK+HFF组）、Wistar+HFF组、GK高脂喂养后内毒素（LPS）注射组（GK+HFF+LPS组）和Wistar+HFF+LPS组，每组6只。取GK+HFF+LPS组和Wistar+HFF+ LPS组大鼠，分别以5 mg/kg LPS经腹腔注射建立脓毒血症危重病模型，观察大鼠血糖波动情况，Real-Time PCR检测肝脏、肺脏、肾脏和心肌组织中肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）、IL-6和IL-10等炎症细胞因子mRNA的表达。结果 高脂喂养14周后，Wistar组大鼠的体质量明显大于GK组（P<0.05），GK组大鼠血糖浓度显著高于Wistar组（P<0.05）。LPS注射后，GK+HFF+LPS组大鼠血糖浓度上升趋势明显，而Wistar+HFF+LPS组大鼠的血糖浓度无明显变化。炎症细胞因子mRNA表达的检测结果显示：Wistar+HFF+LPS组显著高于Wistar+HFF组（P<0.05），GK+HFF+LPS组显著高于GK+HFF组（P<0.05）；Wistar+HFF+LPS组炎症细胞因子mRNA表达的上升幅度与GK+HFF+LPS组比较，差异无统计学意义（P>0.05）。结论 正常大鼠经高脂膳食诱发肥胖后，对急性炎症刺激出现一定的耐受性，且较2型糖尿病大鼠更为显著。

关键词: 糖尿病, 肥胖, 应激性高血糖, 炎症, 2型糖尿病大鼠

Abstract:

Objective To investigate the responsive ability to acute inflammatory stimulus of type 2 diabetic rats and obese non-diabetic rats induced by high-fat diet. Methods Type 2 diabetic GK rats (GK group) and Wistar rats (Wistar group) were fed with high-fat diet for 14 weeks, and the body weight of rats in two groups were observed. Then rats were allocated to GK high-fat feeding baseline group (GK+HFF group), Wistar+HFF group, GK highfat feeding+lipopolysaccharide (LPS) injection group (GK+HFF+LPS group) and Wistar+HFF+LPS group by random number table, with 6 rats in each group. Severe sepsis model was established by intraperitoneal injection of 5 mg/kg LPS in GK+HFF+LPS group and Wistar+HFF+LPS group. The blood glucose fluctuation was observed, and the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-10 mRNA in liver, lung, kidney and myocardial tissues was determined by Real-Time PCR. Results After feeding with highfat diet for 14 weeks, the body weight of rats in Wistar group was significantly higher than that in GK group (P<0.05), and the blood glucose concentration of rats in GK group was significantly higher than that in Wistar group (P<0.05). After LPS injection, the blood glucose concentration of rats in GK+HFF+LPS group significantly increased, while the blood glucose concentration of rats in Wistar+HFF+LPS group did not change significantly. Real-Time PCR revealed that the expression of TNF-α, IL-1β, IL-6 and IL-10 mRNA in Wistar+HFF+LPS group was significantly higher than that in Wistar+HFF group (P<0.05), and the expression of TNF-α, IL-1β, IL-6 and IL-10 mRNA in GK+HFF+LPS group was significantly higher than that in GK+HFF group (P<0.05). The increase of expression of TNF-α, IL-1β, IL-6 and IL-10 mRNA in Wistar+HFF+LPS group was not significantly different from that in GK+HFF+LPS group (P>0.05). Conclusion After feeding with high-fat diet, the tolerance to acute inflammation may increase in obese non-diabetic rats, which is more significant than that in type 2 diabetic rats.

Key words: diabetes mellitus, obesity, stress hyperglycemia, inflammation, type 2 diabetic rat