上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2013, Vol. 33 ›› Issue (6): 833-.doi: 10.3969/j.issn.1674-8115.2013.06.027

• 论著（临床研究） • 上一篇    下一篇

三阴性乳腺癌中p53和Ki67表达的相关研究

徐 婷1，何 奇1，吴克瑾2，邵玉国1，张慧娟3，籍 敏1，王 啸3   

  1. 1.上海交通大学  医学院附属国际和平妇幼保健院乳腺科， 上海 200030； 2.上海交通大学  医学院附属新华医院普外科， 上海 200092； 3.上海交通大学  医学院附属国际和平妇幼保健院病理科， 上海 200030
  • 出版日期:2013-06-28 发布日期:2013-06-28
  • 通讯作者: 吴克瑾, 电子信箱: kejinwu@163.com。
  • 作者简介:徐婷（1980—）, 女， 主治医师， 硕士生; 电子信箱: xanadutt@163.com。

Expression of p53 and Ki67 in triple negative breast cancer

XU Ting1, HE Qi1, WU Ke-jin2, SHAO Yu-guo1, ZHANG Hui-juan3, JI Min1, WANG Xiao3   

  1. 1.Department of Breast Disease, International Peace Maternity & Child Health Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China; 2.Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China; 3.Department of Pathology, International Peace Maternity & Child Health Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China
  • Online:2013-06-28 Published:2013-06-28

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摘要：

目的 观察雌激素受体（ER）、孕激素受体（PR）及人类表皮生长因子受体2 （HER-2）均阴性的乳腺癌（三阴性乳腺癌）中p53和Ki67的表达，并探讨其临床意义。方法 收集病理确诊的浸润性乳腺癌233例，其中三阴性乳腺癌44例（18.88%），非三阴性乳腺癌189例（71.12%），采用免疫组织化学二步法检测乳腺癌组织中p53和Ki67的表达，分析三阴性乳腺癌中p53和Ki67表达与临床病理学特征的关系，并分析两者的相关性及与预后的关系。结果 三阴性乳腺癌组织中p53和Ki67的阳性表达率分别为45.5%和68.2%，显著高于非三阴性乳腺癌组织（20.6%和47.1%）（P<0.05）。p53阳性表达与三阴性乳腺癌的大小、组织学分级、淋巴结转移、脉管内癌浸润有关（P<0.05）；Ki67阳性表达与三阴性乳腺癌的组织学分级有关（P<0.05）。在所有乳腺癌病例中，p53与Ki67表达呈正相关（r=0.294，P=0.000）；在三阴性乳腺癌病例中，p53与Ki67表达不相关（r=0.134，P>0.05）。在所有乳腺癌病例中，p53表达阳性者总体生存率低于p53表达阴性者（P=0.000），Ki67表达阳性者总体生存率低于Ki67表达阴性者（P=0.000）；在三阴性乳腺癌病例中，p53表达阳性者与阴性者以及Ki67表达阳性者与阴性者总体生存率的差异均无统计学意义（P>0.05）。结论 三阴性乳腺癌组织中p53和Ki67的表达水平均上调，但两者无明显相关性。p53和Ki67表达可能与乳腺癌的预后有关，但尚不能指导三阴性乳腺癌的预后判断。

关键词: 乳腺肿瘤, 三阴性乳腺癌, p53, Ki67

Abstract:

Objective To investigate the expression of p53 and Ki67 in breast cancer with negative estrogen receptor (ER), negative progesterone receptor (PR) and negative human epidermal growth factor receptor 2 (HER-2) (triple negative breast cancer), and explore its clinical significance. Methods A total of 233 cases of invasive breast cancer confirmed by pathology were collected, among which 44 (18.88%) were triple negative breast cancer. The expression of p53 and Ki67 in tissues of triple negative breast cancer was detected by immunohistochemical EnVision two-step methods. The tissues of 189 cases of non-triple negative breast cancer were served as controls. The correlation of expression of p53 and Ki67 in triple negative breast cancer with clinicopathological features and their relationship with prognosis were analysed. Results The positive expression rates of p53 and Ki67 in tissues of triple negative breast cancer were 45.5% and 68.2% respectively, which were significantly higher than those in tissues of nontriple negative breast cancer (20.6% and 47.1%) (P<0.05). In triple negative breast cancer, the expression of p53 correlated with tumor size, histological grade, lymph node status and vessel invasion (P<0.05), and the expression of Ki67 correlated with histological grade (P<0.05). The expression of p53 was significantly related to that of Ki67 in all the breast cancer cases (r=0.294, P=0.000), but the expression of p53 was not significantly related to that of Ki67 in triple negative breast cancer cases (r=0.134, P>0.05). In all the breast cancer cases, the overall survival of cases with positive expression of p53 was lower than that of cases with negative expression of p53 (P=0.000), and the overall survival of cases with positive expression of Ki67 was lower than that of cases with negative expression of Ki67 (P=0.000). In triple negative breast cancer cases, there was no significant difference in the overall survival between cases with positive expression of p53 and those with negative expression of p53 (P>0.05) and between cases with positive expression of Ki67 and those with negative expression of Ki67(P>0.05). Conclusion The expression of p53 and Ki67 is up-regulated in tissues of triple negative cancer, while the expression of p53 is not significantly related to that of Ki67. The expression of p53 and Ki67 may be related to the prognosis of breast cancer, while may not indicate the prognosis of triple negative breast cancer.

Key words: breast cancer, triple negative breast cancer, p53, Ki67