上海交通大学学报(医学版)

• 论著(基础研究) • 上一篇    下一篇

T细胞淋巴瘤长链非编码RNA ST20-AS1的表达及其生物学作用

孙漪峰, 许彭鹏, 赵维莅   

  1. 上海交通大学 医学院附属瑞金医院血液科, 上海血液学研究所, 上海 200025
  • 出版日期:2016-09-28 发布日期:2016-10-31
  • 通讯作者: 赵维莅, 电子信箱: zhao.weili@yahoo.com。
  • 作者简介:孙漪峰(1989—), 男, 硕士生; 电子信箱: sunyifeng89@gmail.com。
  • 基金资助:

    国家自然科学基金(81325003, 81520108003, 81201863);上海市科学技术委员会(14430723400, 14140903100);瑞金医院优秀青年教师(800000000003);上海交通大学晨星青年学者计划;上海市青年医师培养资助计划;上海市教育委员会高峰高原学科建设计划(20152208)

Expression and biological effect of long non-coding RNA  ST20-AS1  in T-cell lymphoma

SUN Yi-feng, XU Peng-peng, ZHAO Wei-li   

  1. Shanghai Institute of Hematology, Department of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200025, China
  • Online:2016-09-28 Published:2016-10-31
  • Supported by:

    National Natural Science Foundation of China, 81325003, 81520108003, 81201863; Shanghai Commission of Science and Technology, 14430723400, 14140903100; Ruijin Hospital Outstanding Young Teachers, 800000000003; Shanghai Jiao Tong University Morningstar Award; Shanghai Young Doctor Training Program; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support, 20152208

摘要:

目的·检测T细胞淋巴瘤患者长链非编码RNA(lncRNA)ST20-AS1的表达,并探索其对T细胞淋巴瘤细胞增殖和侵袭性的影响。方法·运用lncRNA芯片筛选出ST20-AS1作为研究的lncRNA,通过芯片数据发现ST20-AS1的高表达与NOTCH信号通路的关系;Real-time RT-PCR方法验证ST20-AS1在T细胞淋巴瘤患者中的表达水平,并验证其对NOTCH通路的激活作用;构建ST20-AS1稳定表达的T淋巴瘤Jurkat细胞株,进行细胞增殖和Transwell侵袭实验,观察细胞增殖和侵袭性的改变。结果·ST20-AS1在T淋巴瘤患者中呈高表达,ST20-AS1高表达患者的NOTCH通路被显著激活。在Jurkat细胞株中发现,ST20-AS1高表达活化NOTCH信号通路,增强细胞增殖和侵袭能力。利用siRNA敲低NOTCH1的表达后,ST20-AS1高表达细胞株的增殖和侵袭能力降低。结论·ST20-AS1激活NOTCH信号通路,促进淋巴瘤细胞的增殖和侵袭性。

关键词: T细胞淋巴瘤, ST20-AS1, NOTCH信号通路, 细胞增殖, 细胞侵袭

Abstract:

Objective·To measure the expression of ST20-AS1, a long non-coding RNA (lncRNA) in patients with T-cell lymphoma, and to explore its effects on the proliferation and invasion of T-cell lymphoma cells. Methods·Study lncRNA was screened using the lncRNA array. The association between high expression of ST20-AS1 and NOTCH signaling pathway was detected via the data from the lncRNA array. The expression level of ST20-AS1 in T-cell lymphoma patients was assessed by real-time RT-PCR and its activation effect on NOTCH signaling pathway was verified. ST20-AS1-overexpressing T-cell lymphoma cell line Jurkat was constructed and cell proliferation test and Transwell assay were performed to observe changes in cell proliferation and invasion. Results·ST20-AS1 was overexpressed and NOTCH signaling pathway was significantly activated in T-cell lymphoma patients. The overexpression of ST20-AS1 activated the NOTCH signaling pathway and promoted lymphoma cell proliferation and invasion in Jurkat. When NOTCH1 was knocked down by siRNA, cell proliferation and invasion was reduced in ST20-AS1-overexpressing cell line Jurkat. Conclusion·ST20-AS1 can activate the NOTCH signaling pathway and promote lymphoma cell proliferation and invasion.

Key words: T-cell lymphoma, ST20-AS1, NOTCH signaling pathway, cell proliferation, cell invasion