上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

CTHRC1在卵巢癌细胞侵袭转移中的作用及相关机制

郭碧莹，严欢，张殊   

  1. 上海交通大学医学院附属仁济医院妇产科，上海市妇科肿瘤重点实验室，上海 200127
  • 出版日期:2017-04-28 发布日期:2017-05-04
  • 通讯作者: 张殊，电子信箱：drzhangshu@126.com。
  • 作者简介:郭碧莹（1991—），女，硕士生；电子信箱：biying_07@163.com。
  • 基金资助:

    国家自然科学基金（81672564）

Effects of CTHRC1 on human ovarian cancer cell metastasis and its mechanism

GUO Bi-ying, YAN Huan, ZHANG Shu   

  1. Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Gynecology Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2017-04-28 Published:2017-05-04
  • Supported by:

    National Natural Science Foundation of China, 81672564

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摘要：

目的 ·探究胶原三股螺旋重复蛋白1（CTHRC1）在卵巢癌细胞侵袭转移中的作用及其相关机制。方法 ·采用Western blotting法检测卵巢癌细胞株中CTHRC1蛋白水平。选取高表达细胞株转染以CTHRC1为靶标的shRNA下调CTHRC1表达水平，以慢病毒lenti-CTHRC1转染低表达细胞株，通过Transwell实验观察细胞过表达或沉默CTHRC1后体外迁移能力和侵袭能力的变化，并检测转移相关蛋白Slug、MMP-2的表达情况。结果 · HO8910细胞中CTHRC1表达量较低，lenti-CTHRC1转染后CTHRC1蛋白水平明显升高，细胞迁移和侵袭能力增强，Slug、MMP-2表达显著增高（均P<0.05）。CaOV3细胞则高表达CTHRC1，转染慢病毒lenti-shCTHRC1后，CTHRC1蛋白水平下调，细胞迁移和侵袭能力明显降低，Slug、MMP-2表达也显著降低（均P<0.05）。结论 · CTHRC1可能通过上调
Slug、MMP-2蛋白表达，进而促进卵巢癌细胞侵袭转移。

关键词: 卵巢癌, CTHRC1, Slug, MMP-2, 细胞迁移

Abstract:

Objective · To investigate the role of collagen triple helix repeat containing-1 (CTHRC1) in ovarian cancer cell metastasis and the related mechanism. Methods · The expression of CTHRC1 in ovarian cancer cells was detected by Western blotting. The cell line which had high expression of CTHRC1 was transfected with a CTHRC1 specific shRNA, and the lenti-CTHRC1 was used to overexpress CTHRC1 in the cell line whose expression of CTHRC1 was very low. Then the expression of Slug and MMP-2 was assessed. Transwell assay was used to determine the migration and invasion capability of ovarian cancer cells after transfection. Results · The expression of CTHRC1 in HO8910 cells was the lowest, while the CTHRC1 protein level was dramatically increased after transfection of lenti-CTHRC1. Meanwhile, there was a distinct rise of the migration and invasion ability, as well as the expression of Slug and MMP-2 (all P<0.05). Conversely, CaOV3 cells had a higher protein expression of CTHRC1. By using lenti-shCTHRC1, a remarkable knockdown of CTHRC1 was obtained. Likewise, the capability of migration and invasion was decreased, and the Slug and MMP-2 expression was reduced (all P<0.05). Conclusion · CTHRC1 might positively regulate Slug and MMP-2 to promote ovarian cancer cell metastasis.

Key words: ovarian cancer, CTHRC1, Slug, MMP-2, cell migration