上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2017, Vol. 37 ›› Issue (7): 901-.doi: 10.3969/j.issn.1674-8115.2017.07.004

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TACI-Ig 融合蛋白对小鼠视神经病理损伤的影响

韩露 1，杨红 1，李梅 1，朱德生 2，李则挚 2，刘明媛 1，管阳太 1, 2   

  1. 1. 第二军医大学附属长海医院神经内科，上海 200433；2. 上海交通大学  医学院附属仁济医院神经内科，上海 200001
  • 出版日期:2017-07-28 发布日期:2017-08-25
  • 通讯作者: ?管阳太，电子信箱：yangtaiguan@sina.com
  • 作者简介:韩露（1990—），女，硕士生；电子信箱：hlkcn1990@163.com
  • 基金资助:
    国家自然科学基金（81200924, 81230027，81401127）；上海市卫生和计划生育委员会基金（201440347，20134Y044）；上海交通大学医工交叉基金 （YG2016MS48）

Pathological damage of optic nerve in mice by the application of TACI-Ig#br#

HAN Lu1, YANG Hong1, LI Mei1, ZHU De-sheng2, LI Ze-zhi2, LIU Ming-yuan1, GUAN Yang-tai1, 2   

  1. 1. Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China
  • Online:2017-07-28 Published:2017-08-25
  • Supported by:
    National Natural Science Foundation of China, 81200924, 81230027, 81401127; Foundation of Shanghai Municipal Commission of Health and Family Planning, 201440347, 20134Y044; Medical Engineering Foundation of Shanghai Jiao Tong University, YG2016MS48

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摘要： 目的 · 探讨腹腔注射 TACI-Ig 融合蛋白对小鼠视神经炎症浸润、髓鞘完整性等的影响。方法 · 将 24 只 C57BL/6J 小鼠随机分 为 4 组，分别为空白对照组、生理盐水注射组、TACI-Ig 低剂量注射组（0.4 mg/kg）、 TACI-Ig 高剂量注射组（4 mg/kg），每组6 只。 TACI-Ig 隔日腹腔注射 1 次，生理盐水对照组按照同样方式注射生理盐水 0.2 mL，空白对照组不给予任何干预。20 日后将小鼠麻醉灌 注，取眼球和视神经组织，石蜡包埋，切片后行苏木精 - 伊红（H-E）染色和固蓝（LFB) 染色，分别观察炎症细胞浸润情况和髓鞘 脱失情况。结果 · H-E 染色可见空白对照组和生理盐水对照组着色均匀，视神经纤维排列紧密、规则；TACI-Ig 低剂量注射组（0.4 mg/ kg）视神经结构与两对照组类似；高剂量注射组（4 mg/kg）细胞均匀分布，可观察到炎症细胞少量浸润，炎症细胞浸润评分与其他组 比较无明显差异（P=0.610 3）。所有组别视网膜结构清晰、层次分明，神经节细胞单层紧密排列、胞体完整、胞核可见、分布均匀， 各组实验动物视网膜各层结构相比较并无明显差异。经 LFB 染色后光学显微镜下观察，发现视神经髓鞘均无明显脱失和损伤情况，4 组之间比较差异无统计学意义（P=0.473 6）。 结论 · 低剂量（0.4 mg/kg）TACI-Ig 腹腔注射对视神经、视网膜神经节细胞无损伤，高 剂量（4 mg/kg）可能引起视网膜区域炎症少量浸润。

关键词: &ensp, TACI-Ig 融合蛋白, B 淋巴细胞刺激因子, 视神经, 脱髓鞘

Abstract:

Objective · To eliminate the effects of intraperitoneal injection of transmembrane activator and calcium modulator and cyclophilin ligand interactor-Ig (TACI-Ig) on the opitc neuritis and the integrity of myelin sheath in mice.  Methods · Twenty-four C57BL/6J mice were randomly divided into 4 groups, which were blank control group, saline control group, low-dose (0.4 mg/kg) TACI-Ig group and high-dose (4 mg/kg) TACI-Ig group, with 6 mice in each group. All groups were received intraperitoneal injection every other day. The saline control group received 0.2 mL saline injection in the same way, and the blank control group was not given any intervention. After 20 d of treatment, the eyeballs and optic nerve tissues were collected from each mouse under anaesthesia, embedded in paraffin and stained with hematoxylin-eosin (H-E) and Luxol fast blue (LFB), respectively.  Results · H-E staining indicated that optic nerve fibers arranged closely both in blank and saline control groups and the staining of tissues was uniform. The optic nerve structure of low-dose TACI-Ig group was similar to blank and saline control groups, while in high-dose of TACI-Ig group, the infiltration of inflammatory cells was observed. The inflammatory cell infiltration scores were not significantly different in all groups (P=0.610 3). The retinal structures of all groups were clear and distinct to observe, and single ganglion cells arranged tightly with complete cell shape, visible nucleus and uniform distribution. There was no difference in the retina structure among 4 groups. LFB staining indicated that there was no significant loss of the optic nerve myelin in 4 groups by microscope observation (P=0.473 6).  Conclusion · Low-dose (0.4 mg/kg) TACI-Ig injection wouldn’t damage the normal structure of optic nerves and retinal ganglion cells, meanwhile high-dose (4 mg/kg) of TACI-Ig injection might cause minor infiltration of inflammatory cells into retina.

Key words:  TACI-Ig, B lymphocyte stimulator, optic nerve, demyelination