上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (12): 1420-.doi: 10.3969/j.issn.1674-8115.2018.12.004

• 论著·基础研究 • 上一篇    下一篇

利拉鲁肽对高糖诱导内皮细胞 miRNA -146b-3p、炎症因子及环氧化酶 -2表达的影响

苏征佳，潘晓茜，曹久妹，吴方   

  1. 上海交通大学医学院附属瑞金医院老年病科，上海 200025
  • 出版日期:2018-12-28 发布日期:2019-01-27
  • 通讯作者: 吴方，电子信箱：wf10428@rjh.com.cn。
  • 作者简介:苏征佳（1985—），女，主治医师，硕士；电子信箱： szj11699@rjh.com.cn。
  • 基金资助:
    上海市卫生和计划生育委员会科研课题（20164Y0233）；上海市科学技术委员会课题（ 16ZR1430900）

Effects of liraglutide on glucose induced of miRNA-146b-3p, inflammatory factors and COX-2 in human umbilical vein endothelial cells

SU Zheng-jia, PAN Xiao-qian, CAO Jiu-mei, WU Fang   

  1. Department of Geratology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2018-12-28 Published:2019-01-27
  • Supported by:
    Research Project of Shanghai Health and Family Planning Commission, 20164Y0233; Subject of Shanghai Municipal Committee of Science and Technology, 16ZR1430900

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摘要： 目的 ·探讨利拉鲁肽对高糖诱导人脐静脉内皮细胞（ human umbilical vein endothelial cells，HUVECs）miR-146b-3p及白细胞介素 -6（interleukin-6，IL-6）、肿瘤坏死因子 α（tumor necrosis factor-α，TNF-α）、环氧化酶 -2(cyclooxygenase-2，COX-2)表达的影响。方法 ·高糖组和对照组 HUVECs分别以高浓度葡萄糖（ 25 mmol/L）及正常浓度葡萄糖（ 7 mmol/L）处理 24 h，再以利拉鲁肽分别干预上述 2组细胞。定量聚合酶链反应（ quantitative polymerase chain reaction，qPCR）检测各组 miR-146b-3p、IL-6、TNF-α、COX-2的表达变化。抑制 miR-146b-3p后观察炎症因子及 COX-2的表达变化。结果 ·在高糖诱导的 HUVECs中，miR-146b-3p的表达下降， IL-6、TNF-α及 COX-2的表达均升高。抑制 miR-146b-3p表达会显著升高高糖诱导的 HUVECs中 IL-6、TNF-α及 COX-2的表达。利拉鲁肽可显著升高高糖诱导的 HUVECs中 miR-146b-3p的表达，降低 IL-6、TNF-α及 COX-2的表达。结论 ·在高糖环境下，利拉鲁肽可能通过升高 miR-146b-3p的表达而降低 IL-6、TNF-α及 COX-2的表达，从而改善炎症和血管内皮功能。

关键词: 利拉鲁肽, miR-146b-3p, 炎症因子, 环氧化酶 -2, 糖尿病

Abstract:

Objective · To investigate the effects of liraglutide on glucose induced of miRNA-146b-3p, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2) in human umbilical vein endothelial cells (HUVECs). Methods · HUVECs were grown in the medium with glucose of high concentration (25 mmol/L) or normal concentration (7 mmol/L) for 24 h, then stimulated with liraglutide. Quantitative polymerase chain reaction (qPCR) was performed to detect the of miR-146b-3p, IL-6, TNF-α and COX-2. The of IL-6, TNF-α and COX-2 were detected after HUVECs were transfected with anti-miR-146b-3p. Results · The of miR-146b-3p was decreased in high glucose induced cells, while the of IL-6, TNF-α and COX-2 were increased. Silenced of miR-146b-3p increased the of IL-6, TNF-α and COX-2. Liraglutide increased the of miR-146b-3p and decreased the of IL-6, TNF-α and COX-2 in high glucose induced cells. Conclusion · Liraglutide may relieve inflammation and improve vascular endothelial function in hyperglycemia condition through regulating miR146b-3p to decrease of IL-6, TNF-α and COX-2.

Key words: liraglutide, miR-146b-3p, inflammatory factor, cyclooxygenase-2 (COX-2), diabetes

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