上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

去铁胺对大鼠脑外伤急性期脑红蛋白表达及损伤灶的影响

王凯，荆尧，徐晨，金芝萍，王敢，曹合利，陈世文   

  1. 上海交通大学附属第六人民医院神经外科， 上海 200233
  • 出版日期:2016-03-28 发布日期:2017-06-02
  • 通讯作者: 陈世文, 电子信箱: chenshiwen@126.com。
  • 作者简介:王 凯(1990—)， 男， 硕士生; 电子信箱: wkai852@126.com。

Effects of deferoxamine on neuroblobin expression and lesions in rats with traumatic brain injury in acute stage

WANG Kai, JING Yao, XU Chen, JIN Zhi-ping, WANG Gan, CAO He-li, CHEN Shi-wen   

  1. Department of Neurosurgery, Shanghai Sixth Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2016-03-28 Published:2017-06-02

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摘要：

目的 探讨去铁胺（DFX）对大鼠脑外伤急性期半影区脑红蛋白（Ngb）表达及损伤灶的影响。方法 90只SD大鼠随机分为假手术组、实验组和对照组,每组30只。实验组和对照组大鼠分别建立控制性皮质撞击模型，假手术组开颅而不致伤。实验组大鼠伤后2、6 h经腹腔注射DFX（100 mg/kg），此后每12 h给药1次；对照组大鼠在相同时间点经腹腔注射等量生理盐水。各组大鼠分别于术后6、12、24、48 h取6只断头取脑，RT-PCR及Western blotting检测损伤半影区脑组织Ngb表达。伤后3 d，每组各取6只大鼠行3.0T磁共振成像（MRI）检查，选T2序列计算损伤灶体积。结果 损伤半影区脑组织Ngb mRNA和蛋白表达在伤后早期显著增高，分别在12 h和24 h达高峰，随后缓慢下降，直到伤后48 h仍维持较高的表达水平。与对照组比较，实验组大鼠伤后12、24、48 h的Ngb mRNA和蛋白表达明显上调，伤后3 d损伤灶体积较小。结论 DFX在脑外伤急性期有脑保护作用，能缩小损伤灶体积，其保护作用可能与诱导内源性脑保护因子Ngb的表达有关。

关键词: 去铁胺, 脑外伤, 脑红蛋白, 损伤灶体积

Abstract:

Objective To investigate effects of deferoxamine (DFX) on neuroblobin (Ngb) expression in cerebral penumbra and lesions in rats with traumatic brain injury (TBI) in acute stage. Methods Ninety SD rats were randomly assigned to sham operation group (n=30), experimental group (n=30) and control group (n=30). A controlled cortical impact (CCI) model was established for experimental group and control group. Rats in the sham group underwent the same surgical procedure without injury. Rats in the experimental group were intraperitoneal injected with DFX (100 mg/kg) at 2 and 6 h after TBI and were administrated once every 12 h afterward. Rats in the control group were intraperitoneal injected with the same volume of saline at same time points. At 6, 12, 24 and 48 h after surgery, six rats from each group were sacrificed and brains were harvested. The Ngb expression in cerebral penumbra was detected by RT-PCR and Western blotting. Six rats from each group underwent MRI examination at 3.0T at 3 d after TBI and lesion volumes were calculated on the basis of T2weighted images. Results Ngb mRNA and protein expressions in penumbra significantly increased in early stage after TBI, reached peaks at 12 and 24 h, respectively, and then decreased slowly and maintained fairly high levels until 48 h after TBI. Ngb mRNA and protein expressions at 12, 24 and 48 h after TBI in the experimental group were significantly higher than those in the control group. Lesion volumes at 3 d after TBI in the experimental group were smaller than those in the control group. Conclusion DFX has neuroprotective effect in acute stage after TBI. It can reduce the lesion volume and the protective effect may be associated with inducing the expression of endogenous brain protection factor Ngb.

Key words: deferoxamine, traumatic brain injury, neuroglobin, lesion volume