C型凝集素受体和Toll样受体在子前期重度患者胎盘中的表达
网络出版日期: 2011-08-29
基金资助
上海市科委基金(10411966500)
Expression of C-type lectin receptors and Toll-like receptors in placenta of severe pre-eclampsia
Online published: 2011-08-29
Supported by
Shanghai Science and Technology Committee Foundation, 10411966500
目的 分析子前期(PE)患者胎盘组织中C型凝集素样受体家族中DC-SIGN和甘露糖受体(MR)以及Toll样受体(TLR)家族中TLR-2和TLR-4的表达情况。方法 采集PE重度患者(PE组,n=20)及正常妊娠妇女(NP组,n=20)的新鲜胎盘标本;采用免疫组织化学技术对胎盘组织中DC-SIGN、MR、TLR-2和TLR-4的表达进行定位分析;采用Western blotting法检测胎盘组织中的DC-SIGN、MR、TLR-2和TLR-4的蛋白表达。结果 两组胎盘组织中均有DC-SIGN、MR、TLR-2和TLR-4的表达,主要位于胎盘合体滋养层细胞,在细胞滋养层细胞中亦有少量表达。PE组与NP组比较,胎盘组织TLR-4阳性细胞表达率明显升高(P<0.01);DC-SIGN、MR阳性细胞表达率明显降低(P<0.01,P<0.05),TLR-2阳性细胞表达率的差异无统计学意义(P>0.05)。结论 相对于正常晚期妊娠妇女,PE患者胎盘组织中C型凝集素受体DC-SIGN和MR表达减少,TLR-4过度表达;两种受体家族活化平衡失调可能是PE发病机制中的关键环节之一。
李 黎, 徐 亮, 滕银成 . C型凝集素受体和Toll样受体在子前期重度患者胎盘中的表达[J]. 上海交通大学学报(医学版), 2011 , 31(8) : 1145 . DOI: 10.3969/j.issn.1674-8115.2011.08.022
Objective To investigate the expression of C-typed lectin receptors of dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) and mannose receptor (MR) and Toll-like receptors (TLR) of TLR-2 and TLR-4 in placenta tissues in patients with pre-eclampsia (PE). Methods The placenta tissues were obtained from patients with severe pre-eclampsia (PE group, n=20) and normal pregnant women (NP group, n=20). The expression of DC-SIGN, MR, TLR-2 and TLR-4 in placenta tissues was localized by immunohistochemistry, and the expression of DC-SIGN, MR, TLR-2 and TLR-4 protein in placenta tissues was detected by Western blotting. Results There was expression of DC-SIGN, MR, TLR-2 and TLR-4 in syncytiotrophoblasts and cytotrophoblasts of placenta tissues in both groups, and the majority was in syncytiotrophoblasts. The expression of TLR-4 in placenta tissues in PE group was significantly higher than that in NP group (P<0.01), the expression of DC-SIGN and MR in placenta tissues in PE group was significantly lower than that in PE group (P<0.01, P<0.05), and there was no significant difference in the expression of TLR-2 in placenta tissues between two groups (P>0.05). Conclusion Compared with normal pregnant women, the expression of C-typed lectin receptors of DC-SIGN and MR is lower, while the expression of TLR-4 is abnormally higher in placenta tissues in patients with PE. The imbalance between these two receptors may play an important role in the pathogenesis of PE.
Key words: pre-eclampsia; placenta; C-type lectin receptors; Toll-like receptors
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