Objective To study the effect of huachansu (HCS) injection and AD injection on treatment of advanced colorectal cancer. Methods Sixty patients with advanced colorectal cancer were randomly assigned into 2 groups. The HCS group (30 cases) was treated with HCS injection and basal therapy; and the AD group (30 cases) was treated with AD injection and basal therapy. All the patients received 4 treatment courses which were of 10 d and 2 weeks off as one course. The traditional Chinese medicine syndrome, quality of survival, NK and T cells, serum CEA and CA199 levels were observed. Results In AD group, patients got remarkable improvement in joy for hot and pressing, fatigue, sallow complexion, and pale tongue with fat tongue body and white fur after treatment, and there were significant differences compared to HCS group (P<0.05). The improvement rate and total effective rate were 46.67% and 76.67% in AD group, which were better than 23.33% and 63.33% in HCS group (P<0.05). For improvement of immune function, the immunological indexes of CD3, CD4, the percentage of NK cells and CD4/CD8 of patients in two groups improved markedly in comparison to before treatment (P<0.05), but there were no statistical differences between two groups (P>0.05). However, the of serum tumor markers of CEA and CA199 had better improvement in HCS group (P<0.05). Conclusion In treatment on advanced spleenqi deficiency colorectal cancer patients, HCS injection and AD injection had good curative effect. AD injection improved patient's syndromes and quality of life, and HCS injection improve the expression of serum tumor markers CEA and CA199, whose internal mechanism needs further research.