Abstract:

Objective To explore the gene types of HPV that infects the genital tract of women and the relationships of multiple infections, hr-HPV load, integrated state of HPV16, and the severity of cervical lesions. Methods A total of 1 082 patients who received treatment in the Department of Obstetrics and Gynecology of Sixth People's Hospital, Shanghai Jiao Tong University during the period of 2009.1.1-2011.12.31 were selected. PCR-reverse dot blot, HC-Ⅱ, and multiplicitas real time PCR were adopted to detect the gene types of HPV that infected the genital tract of patients, hr-HPV load, and integrated state of HPV16. Results Nineteen gene types of HPV were detected from the genital tract of patients. Among 379 patients who were infected by HPV (35.03%), 310 of them were infected by hr-HPV (28.65%). The most common HPV types were HPV16, HPV11, HPV6, HPV52, HPV58, and HPV18. Their infection rates were 12.57%, 8.32%, 7.95%, 6.56%, 5.54%, and 4.71%, respectively. The higher the hr-HPV load of the genital tract of patients and the multiple infection rate, the risk of suffering serious cervical diseases was higher (P<0.05，P<0.001). The proportion of HPV16 integration in cervical tissue cells was positively correlated to the severity of cervical lesions (r=0.812，P<0.001). Conclusion The most common hr-HPV types that threat the female reproductive health are HPV16, HPV18, HPV52, and HPV58. The multiple infection rate of HPV in the genital tract, loading dose of hr-HPV, and proportion of HPV16 integration are high risk factors of suffering cervical precancerosis and cancer.

Key words: human papillomavirus, cervical lesions, genetype, loading dose, integrated state