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Changes of expression of complement receptor 1 protein of patients with cerebral amyloid angiopathy

WANG Cheng1, CHU Xing-kun2, LI Gen1, MA Jian-fang1   

  1. 1.Institute of Neurology， Department of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Shanghai Institute for Biological Science, Chinese Academy of Science, Shanghai 200025, China
  • Online:2015-11-28 Published:2016-01-13
  • Supported by:

    Young Scienticts Project of National Natural Science Foundation of China， 81200979

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Abstract:

Objective  To observe the expression of complement receptor 1 (CR1) protein of patients with cerebral amyloid angiopathy (CAA). Methods  APP-PS1 transgenic mice (Tg6799) were used as the animal model of CAA. The hippocampus and temporal cortex tissues of mice were harvested and the CR1 protein was detected by Western blotting and enzyme-linked immune sorbent assay (ELISA). CD31 was regarded as the marker of vascular endothelial cells and co-localization of CR1 and CD31 was performed by immunofluorescence. Changes of the expression of CR1 protein in blood vessels were observed. Aβ40 was regarded as the marker of CAA and immunofluorescence staining of CR1 and Aβ40 was performed by immunofluorescence. The expression of CR1 of Tg6799 mice was observed. Results  The results of Western blotting and ELISA showed that the expression of CR1 protein of APP-PS1 transgenic mice was significantly higher than that of normal mice. The results of immunofluorescence showed that CR1 protein in blood vessels of APP-PS1 transgenic mice was granular or lumpish and Tg6799 mice expressed CR1 protein. Conclusion  CR1 may play an important role in the pathogenesis of CAA.

Key words: CR1, cerebral amyloid angiopathy, Alzheimer's disease