Abstract:

Objective To investigate changes in proliferation and migration in vascular endothelial cells (VECs) with over-expressed survivin (Svv) gene. Methods Rat aorta abdominalis segments were harvested and stable VECs were obtained by primary cell culture. Cell types were identified by observing cell shapes and using CD31 immunochemistry. VECs were assigned to three groups, i.e. blank control group (bc group), negative control group (nc group), and experiment group (svv group). The bc group used the serum free basic medium. The nc group and the svv group were transfected with empty virus vector and adenovirus carrying Svv gene, respectively. Changes of the Svv protein level in three groups were detected by Western blotting. The proliferation and migration of endothelial cells were measured by CCK8 assay and Transwell test, respectively. Aurora B, phosphorylated Aurora B, and Incenp levels were detected by Western blotting. Results The Svv protein level (P=0.000), proliferation, and migration of rat VECs were increased after being transfected with adenovirus carrying  Svv gene. Aurora B (P=0.000) and Incenp (P=0.000) levels and phosphorylation level of Aurora B (P=0.000) in the svv group were significantly increased as compared with the bc group and the nc group. Conclusion Over-expression of Svv gene can improve the proliferation and migration of rat VECs.

Key words: survivin, endothelial cells, Aurora B, Incenp, chromosomal passenge complex, mitosis