›› 2019, Vol. 39 ›› Issue (10): 1148-.doi: 10.3969/j.issn.1674-8115.2019.10.008

• Original article (Basic research) • Previous Articles     Next Articles

Evaluation of SDF-1 complex with CGF applied to canine dental-pulp-like tissue regeneration

QIAO Lu1, WANG Hui-hui1, QI Ying2, FU Meng-chen1, YAN Yan-hong1, ZHAO Yu-mei1   

  1. 1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Pediatric Dentistry, School of Stomatology, Tongji University, Shanghai 200072, China; 2. Jiading District Odontopathy Dispensary, Shanghai 201800, China
  • Online:2019-10-28 Published:2019-11-22
  • Supported by:
    Project of Science and Technology Commission of Shanghai Municipality,14411963600

Abstract: Objective · To evaluate the feasibility of immature permanent teeth pulp regeneration with a new method that utilizes the integration of concentrated growth factor (CGF) as a scaffold and stromal cell-derived factor-1 (SDF-1). Methods · Canine dental pulp cells (DPC) were isolated and cultured in vitro. Then the effects of SDF-1 and CGF were observed on DPC proliferation and differentiation. The pulpless model was established on the beagle’s immature incisors which were divided into four groups: natural pulp (A), empty cannel (B), CGF-filling (C) and SDF-1/CGF-filling (D). After 10 weeks, specimens were checkedimaging examination, RT-PCR and histological observation. Results · CGF extraction (CGFe) induced DPC proliferation while the combination of SDF-1 and CGFe enhanced this effect and also facilitated odontogenic and angiogenic differentiation of DPC. According to imaging examination, the apex growth of all four groups was in varying degrees. RT-PCR indicated the s of odontogenesis and angiogenesis related genes in group D were higher than those in group C. Besides, neonatal dentin and dental-pulp-like tissue were observed inside the canal of both group C and D, while only cementum-like tissue existed around root apex of group B. Conclusion · SDF-1 plays an important role in driving the process of pulp-like-tissue regeneration of immature permanent teethusing CGF as an effective scaffold.

Key words: immature permanent teeth, pulp regeneration, stromal cell-derived factor-1 (SDF-1), concentrated growth factor (CGF)

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