Effect of miR-877-3p on proliferation of bone marrow mesenchymal stem cells in osteoporosis

doi:10.3969/j.issn.1674-8115.2021.07.006

Abstract

Abstract: Objective

·To investigate the effect of miR-877-3p on the proliferation of bone marrow mesenchymal stem cells (BMSCs) during the occurrence and development of osteoporosis.

Methods

·Twenty female healthy mice aged 8 weeks underwent bilateral ovariectomy to establish the postmenopausal osteoporosis models (OVX group). In addition, 20 healthy female mice were selected in the same period and the adipose tissue near the ovary was removed to establish sham operation models (Sham group). Two months after surgery, bone parameters of the mice, including bone volume fraction (BVF), trabecular number (Tb.N), and bone mineral density (BMD) were detected by micro-CT, and cell proliferation ability of the two groups was detected by CCK-8 and cell counting. The expression of miR-877-3p in the two groups was detected by real-time PCR. After up-regulating (down-regulating) the level of miR-877-3p in the BMSCs, cell proliferation was detected by CCK-8 and cell counting.

Results

·The micro-CT results showed that there were significant differences in bone parameters between the Sham group and the OVX group (P<0.05). The results of CCK-8 and cell counting showed that the proliferation ability of BMSCs in the Sham group was significantly higher than that in the OVX group (P<0.05). Real-time PCR results showed that the expression of miR-877-3p in BMSCs in the Sham group was significantly lower than that in the OVX group (P<0.05). After upregulation (downregulation) of miR-877-3p, the proliferation ability of BMSCs decreased (increased).

Conclusion

·In postmenopausal osteoporosis mice, the proliferation ability of BMSCs is regulated by miR-877-3p, which may be one of the important factors affecting the incidence of osteoporosis.

Key words: bone mesenchymal stem cells, proliferation, miR-877-3p, menopause

CLC Number:

R392.6

Xu TONG, Lin-jing SHU. Effect of miR-877-3p on proliferation of bone marrow mesenchymal stem cells in osteoporosis[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(7): 884-890.

Figures/Tables 8

Fig 1 Flow cytometry analysis of BMSCs

Fig 2 Comparison of micro-CT imaging features of femurs and bone parameters between the two groups

Fig 3 Comparison of osteogenic ability of BMSCs between the two groups

Fig 4 Comparison of adipogenic ability of BMSCs between the two groups

Fig 5 Comparison of expression levels of Runx2 and PPARγ2 in BMSCs between the two groups

Fig 6 Comparison of proliferation ability of BMSCs between the two groups

Fig 7 Expression of miR-877-3p in BMSCs after transfection

Fig 8 Comparison of proliferation ability of BMSCs in the groups after transfection of miR-877-3p

TrendMD

References 21

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