JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (9): 1267-1271.doi: 10.3969/j.issn.1674-8115.2021.09.021

• Review • Previous Articles    

Research progress of artificial oocyte activation technology in clinical application

Pei-qin CHEN(), Di SUN, Yue FU, Qi-meng XIAO, Ming-zhu BAI, Zhen-bo ZHANG()   

  1. Department of Gynecology and Obstetrics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China
  • Received:2020-07-17 Online:2021-08-09 Published:2021-08-09
  • Contact: Zhen-bo ZHANG;
  • Supported by:
    Medicine and Engineering Interdisciplinary Research Fund of Shanghai Jiao Tong University(YG2016MS27)


Oocyte activation deficiency (OAD) is a common cause of failure of intracytoplasmic sperm microinjection (ICSI). The occurrence of OAD is usually related to the inability of sperm to produce sufficient amount of phospholipase C-ζ (PLCζ), causing calcium (Ca2 +) oscillation to activate oocytes. In order to solve OAD and improve the success rate of ICSI, the research and clinical application of artificial oocyte activation (AOA) are becoming more and more extensive. However, its biological safety has not been fully determined. It is necessary to clarify the role of Ca2 + in oocyte activation, summarize the principles of different activators and their current status in clinical application, and explore the safety and application prospect of AOA technology.

Key words: artificial oocyte activation (AOA), oocyte activation deficiency (OAD), intracytoplasmic sperm microinjection (ICSI), calcium oscillation

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