Original article (Basic research)

Inhibition of the lipogenesis of hepatocellular carcinoma HepG2 cells by miR-143 via suppressing FASN

  • LI Wei-ping ,
  • LI Jin-ping ,
  • LI Yuan-hong ,
  • et al
Expand
  • 1.Department of Pharmacology, 2.Department of Biochemistry and Molecular Biology, 3.Department of Pathophysiology, Shanxi Medical University Fenyang College, Fenyang 032200, China

Online published: 2015-03-26

Supported by

Hepatitis Prevention and Control Foundation of China, TQGB20120013

Abstract

Objective To investigate the effects of miR-143 on the lipogenesis of hepatocellular carcinoma HepG2 cells and the molecular mechanism. Methods Expressions of miR-143 of the clinical hepatocarcinoma tissue, para cancer tissue, hepatocellular carcinoma cell line, and normal liver cell line were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The effect of miR-143 on the lipogenesis of hepatocellular carcinoma cells was detected by the oil red O staining assay. The effect of miR-143 on the expression of fatty acid synthase (FASN) of hepatocellular carcinoma cells was detected by the Western blotting. The effects of miR-143 and its inhibitor on the activity of reporter gene vector FASN-CDS were detected by the reporter gene assays. Results The results of qRT-PCR showed that the expression of miR-143 of the clinical hepatocarcinoma tissue and HepG2 cells significantly lower than that of the para cancer tissue and Chang liver cell line (P<0.01). The results of oil red O staining assay indicated that miR-143 significantly decreased the lipid formation of hepatocellular carcinoma cells. The results of qRT-PCR and Western blotting showed that miR-143 significantly decreased mRNA and protein levels of FASN. The results of reporter gene assays indicated that miR-143 in HepG2 cells significantly decreased the activity of FASN-CDS (P<0.01), while the inhibitor of miR-143 significantly increased the activity of FASN-CDS (P<0.01). The inhibitor of miR-143 in HepG2 cells was able to up-regulate the expression of FASN and enhance the lipogenesis. Conclusion MiR-143 can inhibit the lipogenesis of hepatocellular carcinoma cells by suppressing the expression of FASN.

Key words: miR-143; hepatoma; lipogenesis; FASN

Cite this article

LI Wei-ping , LI Jin-ping , LI Yuan-hong , et al . Inhibition of the lipogenesis of hepatocellular carcinoma HepG2 cells by miR-143 via suppressing FASN[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2015 , 35(3) : 337 . DOI: 11.3969/j.issn.1674-8115.2015.03.007

Outlines

/