Original article (Clinical research)

Expression and clinical significance of Pokemon in gastric carcinoma tissue

  • HUO Hua ,
  • CHEN Hong-xing ,
  • WANG Ping
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  • 1.Department of Oncology, 2.Department of Pediatrics, Dongming People's Hospital, Heze 274000, China; 3.Department of Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Online published: 2015-03-26

Abstract

Objective To detect the expression of Pokemon gene in gastric carcinoma tissue and adjacent normal gastric mucosa tissue and analyze the relationship of the expression of Pokemon gene and cinlicopathologic features. Methods Immunohistochemical staining with Eli Vision method was adopted to detect the expression of Pokemon protein in gastric carcinoma tissue of 75 cases and adjacent normal gastric mucosa tissue of 35 cases. The mRNA expression of Pokemon in fresh gastric cancer tissue and adjacent normal gastric mucosa tissue of 30 cases was detected by the reverse transcriptase-polymerase chain reaction (RT-PCR). Results The results of immunohistochemical analysis showed that the positive expression rates of Pokemon protein in gastric cancer tissue and adjacent normal gastric mucosa tissue were 78.7% (59/75) and 34.3% (12/35), respectively. The difference was statistically significant (P<0.05). The results of RT-PCR showed that the mRNA expression levels of Pokemon in fresh gastric cancer tissue of 30 cases were significantly higher than those of adjacent normal gastric mucosa tissue. The differences were statistically significant (P<0.05). The expression of Pokemon in gastric carcinoma tissue was relevant to infiltration depth, clinical stages, and lymph node metastasis (P<0.05). Conclusion The expression of Pokemon plays an important role in the occurrence, development, invasion, and metastasis of gastric carcinoma. Pokemon may become an effective target for the theatment of gastric cancer.

Cite this article

HUO Hua , CHEN Hong-xing , WANG Ping . Expression and clinical significance of Pokemon in gastric carcinoma tissue[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2015 , 35(3) : 418 . DOI: 11.3969/j.issn.1674-8115.2015.03.022

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