Effect of dexmedetomidine on expression of triggering receptor expressed on myeloid cells-1 in lung tissues of rats with sepsis
Online published: 2015-09-30
Supported by
Science and Technology Project Fund of Nantong, HS2013041
Objective To observe the effect of dexmedetomidine on the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in lung tissues of rats with sepsis. Methods Sixty male Sprague Dawley (SD) rats were randomly divided into the sham group (n=15), sepsis group (n=15), dexmedetomidine group (n=15), and yohimbine+ dexmedetomidine group (n=15). The sepsis model was established by cecal ligation and puncture (CLP). The survival rate of rats in 24 h was observed. Serum IL-6, TNF-α, and sTREM-1 levels were measured by enzyme-linked immunosorbent assay (ELISA). The pathological changes of lung tissues were observed and scored. The expression of TREM-1 in lung tissues was detected by real-time PCR and immunohistochemichal method. Results Compared with the sepsis group, the mortality rate and serum IL-6, TNF-α, and sTREM-1 levels of dexmedetomidine group remarkably decreased (P<0.05). The inflammatory response of lung tissue significantly alleviated (P<0.05) and the expression of TREM-1 remarkably decreased (P<0.05). Conclusion Dexmedetomidine can alleviate the inflammatory response of lung tissues of rats with sepsis by inhibiting the expression of TREM-1.
LU Yang , XUE Fei , ZHAO Hong-sheng , et al . Effect of dexmedetomidine on expression of triggering receptor expressed on myeloid cells-1 in lung tissues of rats with sepsis[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2015 , 35(9) : 1274 . DOI: 10.3969/j.issn.1674-8115.2015.09.007
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