Original article (Basic research)

Regulation of aquaporin 1 expression in rat pulmonary microvascular endothelial cells by free fatty acid mixture and action mechanism

  • ZHANG Yi-wei ,
  • TIAN Kun ,
  • WANG Yan ,
  • et al
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  • Department of Anesthesiology, Shanghai Sixth Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200233, China

Online published: 2017-06-02

Supported by

National Natural Science Foundation of China,81272147

Abstract

Objective To investigate the regulation of aquaporin 1 (AQP1) expression in rat pulmonary microvascular endothelial cells (PMVECs) by free fatty acid (FFA) mixture and action mechanism. Methods Western blotting was used to detect changes in AQP1 protein expression in rat PMVECs after FFA treatment for different periods. Western blotting and real-time PCR were used to detect changes in AQP1 protein and mRNA expressions in rat PMVECs after being treated with FFA at different concentrations. The effect of FFA treatment on AQP1 expression was detected by Western blotting after p38 MAPK pathway had been suppressed by SB203580. Results AQP1 protein expressions in RAT PMVECs after being treated with FFA for 6 and 12 h were significantly higher than those in the blank control group (P=0.000,P=0.003). AQP1 protein expressions in 200 μmol/L and 500μmol/L FFA groups were significantly higher than those in the blank control group (P=0.007, P=0.002). AQP1 mRNA expressions in 100 μmol/L, 200 μmol/L and 500μmol/L FFA groups were significantly higher than those in the blank control group (P=0.000). SB203580 pretreatment could suppress FFA-induced increase in AQP1 protein expression in rat PMVECs (P=0.016). Conclusion FFA can up-regulate AQP1 expression and affect AQP1-mediated intrapulmonary water transport progress in fat embolisms to a certain extent. The regulation mechanism may be associated with activation of p38 MAPK signaling pathway.

Cite this article

ZHANG Yi-wei , TIAN Kun , WANG Yan , et al . Regulation of aquaporin 1 expression in rat pulmonary microvascular endothelial cells by free fatty acid mixture and action mechanism[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2016 , 36(3) : 322 . DOI: 10.3969/j.issn.16748115.2016.03.002

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