Objective To assess the effects of Huangmai mixture on the reproductive function of male rodents and protection of the model of yang deficiency. Methods The hydrocortisone-induced mouse model of yang deficiency and emasculation-caused rat model of kidney-yang deficiency were constructed. The rat serum testosterone level, organ coefficients of reproductive organs and glands such as prostate, sperm bladders, and levator ani muscle, sperm motility, and pregnancy rate of female mice were observed after administration of Huangmai mixture. Results For the hydrocortisone-induced mouse model of yang deficiency and emasculation-caused rat model of kidney-yang deficiency, different doses of Huangmai mixture could reduce the level of yang deficiency, significantly increase sperm counts and sperm production without causing the hyperplasia of reproductive organs and glands of male rats, significantly improve the testosterone level and sperm motility, and decrease the sperm abnormality rate of male rats. Conclusion Huangmai mixture can significantly alleviate the symptoms of hydrocortisone-induced mouse model of yang deficiency. For emasculation-caused rat model of kidney-yang deficiency, different doses of Huangmai mixture can reduce the level of kidney-yang deficiency. Huangmai mixture has a mild spermatogenic effect, which can significantly improve the reproductive capacity of male mice.