Objective To investigate the biocompatibility of a developing material prepared by physically mixing poly-p-dioxanone (PPDO) and iohexol. Methods The PPDO and PPDO-iohexol mixture (MIX) were pressed into thin films by a plate vulcanization machine. The haemacompatibility was evaluated through dynamic clotting time test, partial thromboplastin time test, platelet adhesion test, and hemolysis test. The extracts of PPDO and MIX were used to culture human umbilical vein endothelial cells (HUVEC) and human aortic vascular smooth muscle cells (HA-VSMC). Cell proliferation of the two types of cells was detected. The histocompatibility was evaluated through in vivo muscle embed method. Results The differences in both haemacompatibility and histocompatibility between MIX and PPDO were not significant. In the cytocompatibility evaluation, the proliferation rates in HUVEC cultured with extracts of MIX and PPDO were (80.26±8.87)% and (84.59±7.22)% (P=0.391), respectively, while the proliferation rates in HA-VSMC were (94.89±9.64)% and (110.03±13.37)% (P=0.001), respectively.  Conclusion The mixture of PPDO and iohexol has excellent biocompatibility.