Objective·To perform clinical molecular diagnosis for 10 Chinese children with highly-suspected genomic disorders and to evaluate the feasibility of next generation sequencing (NGS) with target capture library in decteting copy number variants (CNVs). Methods·All the patients were evaluated by NGS with SureSelect Inherited Diseases library. The location and size of pathogenic CNVs were identified through bioinformatics analysis. Meanwhile, chromosome microarray analysis (CMA) was used to verify these CNVs. Results·NGS results showed that a 9 345 kb duplication (3 copies) in 17q25.1—q25.3 was detected in chromosome 17 of case 1 and a 8 232 kb heterozygous deletion in 15q11.2—q13.1 was detected in chromosome 15 of case 2. No suspicious CNVs were identified in other 8 cases. The CMA testing results were highly consistent with those of NGS. Conclusion·Two patients were diagnosed with genomic disorders by molecular diagnosis approach. Our research indicates that targeted NGS technique is quite feasible for CNV detection.