Journal of Shanghai Jiao Tong University (Medical Science) >

2019 , Vol. 39 >Issue 2: 120

DOI: https://doi.org/10.3969/j.issn.1674-8115.2019.02.003

Original article (Basic research)

Effect of procyanidin B2 on inflammatory mediators of human periodontal ligament cells

  • GU Li-fen1 ,
  • YANG Tao2 ,
  • GAO Ying3 ,
  • ZHOU Wei1 ,
  • SONG Zhong-chen1
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  • 1. Department of Periodontology, Shanghai Ninth Peoples Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China; 2. The 93313 Army of the Chinese People’s Liberation Army, Changchun 130111, China; 3. The 461 Clinical Department of the 208 Hospital of the Chinese Peoples Liberation Army, Changchun 130021, China

Online published: 2019-03-19

Supported by

National Key R&D Program of China, 2017YFC0840100，2017YFC0840110

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Abstract

Objective · To investigate the effects of procyanidin B2 on the of inflammatory mediators in human periodontal ligament cells (hPDLCs) inducedPorphyromonas gingivalis lipopolysaccharide (LPS). Methods · hPDLCs were cultured using tissue explant method in vitro. The effect of procyanidin B2 on the cell viability of hPDLCs was detectedMTT assay. hPDLCs were stimulatedP. gingivalis LPS after treatment with procyanidin B2 for 1 h. The s of IL-1β, IL-6, and IL-8 mRNA and proteins were detectedreal-time PCR and ELISA assay. Reactive oxygen species (ROS) in the cytoplasm was observed under fluorescence microscope. Nitric oxide (NO) in the supernatant was detectedGriess assay. Results · 100.00 μg/mL procyanidin B2 could enhance the cell viability of hPDLCs. Procyanidin B2 could inhibit the s of IL-1β, IL-6, and IL-8 mRNA and proteins in hPDLCs. It could also downregulate ROS and NO in hPDLCs inducedP. gingivalis LPS. Conclusion · Procyanidin B2 can play an anti-inflammatory roleinhibiting inflammatory mediators in hPDLCs inducedP. gingivalis LPS.

Key words： periodontal ligament cell; procyanidin B2; lipopolysaccharide; inflammatory mediator

Cite this article

GU Li-fen1 , YANG Tao2 , GAO Ying3 , ZHOU Wei1 , SONG Zhong-chen1 . Effect of procyanidin B2 on inflammatory mediators of human periodontal ligament cells[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2019 , 39(2) : 120 . DOI: 10.3969/j.issn.1674-8115.2019.02.003

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