Objective · To propose a method for making organ-on-a-chip based on 3D printing, and study the relationship between cell growth on the chips and various factors. Methods · Through 3D printing technology and surface microstructure transfer method, ulcer-like and ridge-like microstructures of the tumor surface and the intestinal villi were fabricated on a polydimethylsiloxane (PDMS) chip. Combined with fluorescence imaging, the effects of surface modification, shapes and heights of microstructures, and culture time on the surface coverage and density of Caco-2 cells on the chip were measured. Results · The PDMS chip was more likely to induce cell adhesion and growth rather than the 3D printing resin chip. On the surface of three-dimensional structure, cell surface coverage and cell density increased after the surface was treated with rat tail collagen Ⅰ (PP0.000). After surface modification, there was no significant difference in cell density at the same height of different steric structure (P>0.05). Conclusion · The intestinal villi and tumor topological organ chips can be fabricated3D printing technology and surface microstructure transfer method. The surface modification and microstructure height affect the cell growth on the surface.