Objective · To investigate the occurrence of medical staff leaving the COVID-19 isolation room due to discomforts and to provide reference for clinical prevention and treatment. Methods · Stratified sampling method was used to investigate the occurrence of medical staff from Shanghai medical team leaving isolation room earlier due to discomforts, as well as the main symptoms and signs of theirs. Logistic regression was used for risk factor analysis. Results · Among the 227 medical staff working in Leishenshan Hospital in Wuhan, Hubei Province, who were assisted by Shanghai, 69 (30.4%) staff left earlier due to discomforts while working in the isolation room. Two of them had syncope, and sixty-seven of them had symptoms and signs related to presyncope. Symptoms of presyncope include headache, nausea, sweating, dyspnea, and palpitations, etc. Univariate analysis revealed statistically significant differences in occupation (P=0.002), gender (P=0.006), and standing time (P=0.002). Logistic regression analysis showed that occupation (P=0.000), standing time (P=0.025), and hunger (P=0.029) were statistically significant. Conclusion · Different occupation, gender and standing time have different effects on the situation of medical staff leaving the isolation room due to discomforts. Occupation, standing time and feeling of hunger are the influencial factors for medical staff leaving the isolation room earlier.