Journal of Shanghai Jiao Tong University (Medical Science) >

2021 , Vol. 41 >Issue 3: 308 - 313

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.03.004

Basic research

Acetylation involved in bacterial susceptibility to ribosome-targeting antibiotics

  • Ya-nan LAI ,
  • Yu-feng YAO ,
  • Xiao-kui GUO ,
  • Jin-jing NI
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  • 1.Department of Immunology and Microbiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
    2.School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received date: 2020-04-05

  Online published: 2021-04-06

Supported by

National Natural Science Foundation of China(31700121);Innovative Research Team of High-Level Local Universities in Shanghai(SSMU-ZLCX20180202)

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Abstract

Objective

·To investigate the effects of acetylation on the bacterial susceptibility to ribosome-targeting antibiotics.

Methods

·The minimum inhibitory concentrations (MICs) of puromycin, paromomycin, tetracycline and spectinomycin for Salmonella enterica serovar Typhimurium (S. Typhimurium) 14028S were determined by the broth dilution method. The solid mediums with concentration lower than MIC was prepared. The spot dilution assay was employed to determine the susceptibility of S. Typhimuriumstrain 14028S, protein acetyltransferase (pat) knockout strain (Δpat), NAD+-dependent protein deacylase (cobB) knockout strain (ΔcobB) and acetate kinase (ackA) knockout strain (ΔackA) to four kinds of ribosome-targeting antibiotics.

Results

·Increase of acetylation (ΔackA) promoted the susceptibility of S. Typhimurium to puromycin, but promoted the resistance of S. Typhimurium to paromomycin. However, the sensitivity of S. Typhimurium to tetracycline and spectinomycin was not affected by the increase of acetylation (ΔackA or ΔcobB) or decrease of acetylation (Δpat).

Conclusion

·Acetylation is involved in the susceptibility of S. Typhimurium to ribosome-targeting antibiotics, suggesting that acetylation of ribosomal proteins may contribute to bacterial antibiotic sensitivity.

Key words： acetylation; ribosome; ribosome-targeting antibiotics

Cite this article

Ya-nan LAI , Yu-feng YAO , Xiao-kui GUO , Jin-jing NI . Acetylation involved in bacterial susceptibility to ribosome-targeting antibiotics[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2021 , 41(3) : 308 -313 . DOI: 10.3969/j.issn.1674-8115.2021.03.004

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