Journal of Shanghai Jiao Tong University (Medical Science) >

2022 , Vol. 42 >Issue 9: 1336 - 1346

DOI: https://doi.org/10.3969/j.issn.1674-8115.2022.09.022

Public health

Dynamic changes in gut microbiota of women with gestational diabetes mellitus and the correlation with blood glucose, blood lipid and diet

  • Jie WANG ,
  • Hui WU ,
  • Lingpeng LU ,
  • Kefeng YANG ,
  • Jie ZHU ,
  • Hengyi ZHOU ,
  • Die YAO ,
  • Ya GAO ,
  • Yuting FENG ,
  • Yuhong LIU ,
  • Jie JIA
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  • 1.Department of Clinical Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
    2.Department of Clinical Nutrition, College of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    3.Department of Nutrition, Shanghai Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China
    4.Department of Medical Laboratory, Shanghai Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China
    5.Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
    6.School of Family and Consumer Sciences, Texas State University, San Marcos 78666, America
    7.Department of Obstetrics and Gynecology, Shanghai Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China
LIU Yuhong, E-mail: liuyuhong0121@126.com.
JIA Jie, E-mail: jie.jia@shsmu.edu.cn

Received date: 2022-05-18

  Accepted date: 2022-08-19

  Online published: 2022-09-28

Supported by

National Natural Science Foundation of China(81501338);Undergraduate Innovation Program of Shanghai Jiao Tong University School of Medicine(S202010248405);Funding of Shanghai University of Traditional Chinese Medicine(2019LK042)

Fold

Abstract

Objective ·To explore the changes of gut microbiota in the women with gestational diabetes mellitus (GDM) during the second to the third trimesters of pregnancy, and the correlation with dietary intake and glucose-lipid metabolism. Methods ·From June 2019 to January 2021, 78 pregnant women (30 GDM women in the GDM group and 48 healthy women in the control group) in the second trimester of pregnancy were recruited from the Department of Obstetrics and Gynecology of Shanghai Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine. Demographic information and blood biochemical indicators of the two groups were collected and analyzed. A semi-quantified Food-Frequency Questionnaire (FFQ) was used to compare the dietary intake of the two groups of pregnant women. Fecal samples were collected in both groups at the second and the third trimesters of pregnancy, respectively. The characteristics and similarities of gut microbiota in both groups at the second and the third trimesters were compared by 16S rRNA high-throughput sequencing. Spearman correlation heatmap was used to analyze the relation between the abundance of gut microbiota and environmental factors (blood biochemical indicators and dietary intake components) in the pregnant women at the second trimester. Results ·Compared with the control group, the women in the GDM group gained more weight during pregnancy (P=0.012). In the second trimester, compared with the control group, the fast blood glucose level, the blood glucose level at oral glucose tolerance test (OGTT) 1 h and 2 h, and the triacylglycerol level of the pregnant women in the GDM group were higher, and the high-density lipoprotein and the low-density lipoprotein levels were lower (all P<0.05). The results of dietary intake during pregnancy showed that the fat intake level of the pregnant women in the GDM group was higher than that in the control group (P=0.046). From the second to the third trimester of pregnancy, there was no significant difference in the α-diversity and β-diversity of gut microbiota between the GDM group and the control group. The gut bacterial composition at genus level of the GDM group at the second trimester had the least similarity with that of the other three groups (GDM group at the third trimester, control group at the second trimester and the third trimester), while the GDM group and control group had the most similar gut bacterial composition at genus level in the third trimester. Spearman correlation heatmap analysis showed that the abundance of Butyricicoccaceae was positively correlated with dietary fiber and vegetable intake level (r=0.365, P=0.024; r=0.469, P=0.003), the abundance of Klebsiella was positively correlated with triacylglycerol level (r=0.329, P=0.044), and the abundance of Bifidobacterium was negatively correlated with blood glucose level at OGTT 2 h (r=-0.364, P=0.025). Conclusion ·The composition of gut microbiota of pregnant women in the GDM group and control group in the second trimester of pregnancy is significantly different from that in the third trimester of pregnancy. The disorder of gut microbiota related to GDM in the second trimester of pregnancy may be related to glucose-lipid metabolism, dietary fat and vegetable intake level.

Key words： gestational diabetes mellitus (GDM); gut microbiota; diet; glucose-lipid metabolism

Cite this article

Jie WANG , Hui WU , Lingpeng LU , Kefeng YANG , Jie ZHU , Hengyi ZHOU , Die YAO , Ya GAO , Yuting FENG , Yuhong LIU , Jie JIA . Dynamic changes in gut microbiota of women with gestational diabetes mellitus and the correlation with blood glucose, blood lipid and diet[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022 , 42(9) : 1336 -1346 . DOI: 10.3969/j.issn.1674-8115.2022.09.022

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