Journal of Shanghai Jiao Tong University (Medical Science) >

2022 , Vol. 42 >Issue 10: 1361 - 1374

DOI: https://doi.org/10.3969/j.issn.1674-8115.2022.10.001

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Study on the expression of B-cell leukemia/lymphoma 11B in the organ of Corti during mice cochlea development

  • Zhenglin JIANG ,
  • Yunge GAO ,
  • Hao WU
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  • 1.Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
    2.Ear Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China
    3.Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai 200125, China
WU Hao, E-mail: wuhao@shsmu.edu.cn.

Received date: 2022-05-07

  Accepted date: 2022-08-09

  Online published: 2022-12-02

Supported by

National Natural Science Foundation of China(81730028);Science and Technology Innovation Action Plan of Shanghai Municipal Science and Technology Commission(21JC1404000);Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases(14DZ2260300)

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Abstract

Objective ·To explore the expression of B-cell leukemia/lymphoma 11B (BCL11B) in the organ of Corti during mice cochlea development. Methods ·A total of 24 wild-type C57BL/6J mice of random sex at different ages before and after birth [embryonic 14.5 days (E14.5), E15.5, E16.5 and E18.5, and postnatal 0 day (P0), P2, P4 and P7] were selected, with 3 at each age point. After the mice were killed, the bilateral cochleae of the mice were sampled and fixed. The left cochleae were dissected to obtain complete basement membranes, and right cochleae were dehydrated by sucrose solution following cross section. BCL11B antibody was combined with MYOSIN 6 antibody were used for immunohistochemical analysis of whole mount samples of mouse cochlea basement membrane and cross section samples. Confocal fluorescence microscope was used for observing the tissue samples at different age points after staining, and counting the numbers to calculate the percentage of BCL11B-expressing hair cells. Results ·Whole mount samples of mouse cochlear basement membrane and cross section samples at eight age points were successfully prepared. Confocal fluorescence microscope showed that BCL11B expression was first detected specifically in outer three rolls of MYOSIN6 positive hair cells in E15.5 middle-basal turn of mouse cochlea; with the development of mouse cochlea, BCL11B expression gradually strengthened until P2, at which time point BCL11B reached top expression level and started to weaken in P4, with almost no BCL11B expression detected in P7. During the whole period of BCL11B expression in outer hair cells, no BCL11B expression was detected in other cell types in the organ of Corti. The results of the number and percentage of BCL11B-expressing hair cells showed that there was no significant difference among the three age points of E16.5, P0, and P4 (both P>0.05). Conclusion ·It is confirmed that BCL11B is a specifically expressed protein in the early development of outer hair cells in the organ of Corti by co-immunostaining of BCL11B and MYOSIN6.

Key words： organ of Corti; expression pattern; outer hair cell; B-cell leukemia/lymphoma 11B (BCL11B)

Cite this article

Zhenglin JIANG , Yunge GAO , Hao WU . Study on the expression of B-cell leukemia/lymphoma 11B in the organ of Corti during mice cochlea development[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022 , 42(10) : 1361 -1374 . DOI: 10.3969/j.issn.1674-8115.2022.10.001

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