Culture and identification of primary neurons isolated from embryonic ICR mice
Online published: 2013-03-07
Supported by
National Natural Science Foundation of China, 81125003, 30900259; National High-Tech Research and Development Program of China, 863 Program, 2011AA020116; Shanghai Science and Technology Committee Foundation, 12XD1406500, 10140900200; National Basic Research Program of China, 973 Program, 2010CB945200
Objective To establish a favorable primary culture technique for neurons isolated from embryonic ICR mouse cortical tissues. Methods The cortex of embryonic ICR mice aged 13.5 d was isolated, mechanically dissected and digested, and was proceeded to culture. The morphology of neurons was observed, and PCR and Western blotting were applied to identify the expression of Tuj1 and Map2 gene and protein in neurons. Results Cells grew well, with distinct cell body and surrounding bright halation, and there was typical nerve fiber network of synapses. The isolated and cultured cells were confirmed as neurons by PCR and Western blotting. Conclusion This technique is an easy and practical tool for the primary culture of embryonic ICR mouse cortical neurons with high purity.
Key words: neuron; primary culture; embryonic ICR mouse; disease model
CHEN Xue-song, MA Ji, XUE Yan, et al . Culture and identification of primary neurons isolated from embryonic ICR mice[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2013 , 33(2) : 249 . DOI: 10.3969/j.issn.1674-8115.2013.02.025
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