%A HU Chun-xiao, JIANG Dan, WU Shi-yong, GAO Lei, CHANG Guang-lei, TAO Hong-mei, ZHANG Dong-ying %T Relationship between global DNA methylation and hydroxymethylation levels in peripheral blood of elderly patients with myocardial infarction and the degree of coronary atherosclerosis %0 Journal Article %D 2018 %J Journal of Shanghai Jiao Tong University (Medical Science) %R 10.3969/j.issn.1674-8115.2018.07.009 %P 769- %V 38 %N 7 %U {https://xuebao.shsmu.edu.cn/CN/abstract/article_11970.shtml} %8 2018-07-28 %X Objective · To investigate the relationship between the levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with myocardial infarction (MI) and the degree of coronary atherosclerosis. Methods · Forty-three elderly patients with MI were enrolled as the study group and 53 elderly patients with negative findings in coronary angiograms during the same period were collected as the control group. Peripheral blood samples were collected. The levels of global DNA methylation and hydroxymethylation in peripheral blood and the concentration of plasma TET2 were detectedELISA. TET2 mRNA was determinedreal-time qPCR. Results · Compared with the control group, the levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with MI were significantly increased (P0.014, P0.000). The concentration of plasma TET2 and its mRNA in patients with MI were significantly higher than those in the control group (P0.004, P0.028).The levels of global DNA methylation and hydroxymethylation in MI group were significantly correlated with the degree of coronary atherosclerosis (r0.262, P0.017; r0.360, P0.001). Conclusion · The levels of global DNA methylation and hydroxymethylation in peripheral blood of elderly patients with MI are significantly increased, which are significantly correlated with the degree of coronary atherosclerosis.