%A WANG Yun-han1, CHI Ya-nan1, YANG Guan-heng2, FAN Shu-yue1, MA Ji1, XUE Yan1, 2, ZENG Fan-yi1, 2 %T Biological characteristics and gene profile analysis of two kinds of mofetal liver stromal cells %0 Journal Article %D 2019 %J Journal of Shanghai Jiao Tong University (Medical Science) %R 10.3969/j.issn.1674-8115.2019.11.002 %P 1226- %V 39 %N 11 %U {https://xuebao.shsmu.edu.cn/CN/abstract/article_12381.shtml} %8 2019-11-28 %X Objective · To explore the differences in morphology, molecular characteristics and biological functions of different types of mofetal liver stromal cells. Methods · E13.5 mofetal liver stromal cells were obtainedadherent culture, and different cell types were distinguishedmorphology and of surface markers, such as CD44, CD29, CD106, CD45 and Sca-1. Then microarray assay was conductedMoGenome 430 2.0 Array and analyzed at P3 or -2 to identify differential genes. Canonical pathway and biological function analyses were performedusing ingenuity pathway analysis (IPA software). Results · Spindle-like (CD45+CD106-CD29+CD44+Sca-1-) and fibroblast-like (CD45-CD106+CD29+CD44+Sca-1-) fetal liver stromal cells were isolated in this study according to the cell morphology. The 1 485 highly-expressed genes in spindle-like cells mainly involved in immune and inflammation-related signaling pathways; while the 3 374 highly-expressed genes in fibroblast-like cells mainly involved in extracellular matrix formation and cellular adhesion. Conclusion · Mofetal liver stromal cells have strong heterogeneity in biological characteristics and functions, especially in hematopoietic promoting capability.