%A YUAN Yun, ZHANG Hong-ming, HUANG Hui %T Effects of periodontitis on the subsets of helper T cells in peripheral blood of rat models %0 Journal Article %D 2020 %J Journal of Shanghai Jiao Tong University (Medical Science) %R 10.3969/j.issn.1674-8115.2020.10.010 %P 1360-1364 %V 40 %N 10 %U {https://xuebao.shsmu.edu.cn/CN/abstract/article_12778.shtml} %8 2020-10-28 %X Objective · To investigate the effects of periodontitis on the subsets of helper T (Th) cells in peripheral blood of rat models, so as to provide basis for the immunological study of periodontitis. Methods · Rat models of experimental periodontitis were induced by gingiva separation and silk tread ligation. They were divided into periodontitis group (n=5) and normal group (control group, n=5). Periodontal probing was performed two weeks after operation. Alveolar bone resorption was measured by micro-computed tomography (micro-CT). Peripheral blood was taken for flow cytometry to detect the proportions of Th1, Th2, Th17 and T-regulatory (Treg) cells. Difference of cell proportion between the two groups was performed with independent sample t-test. Results · The gingival tissue was swollen and easy to bleed, and alveolar bone resorption was obvious in the periodontitis group. Flow cytometry showed that the proportions of Th1 (P=0.002) and Th17 (P=0.028) cells in peripheral blood of the periodontitis group significantly increased, the proportion of Treg cells significantly decreased (P=0.002), and the proportion of Th2 cells did not change obviously. Conclusion · Periodontitis promotes the differentiation of pro-inflammatory Th cell subsets, while inhibiting the differentiation of inflammation-regulating Th cell subset in peripheral blood of rat models.