%A XIE Chao, WANG Fang-yuan, YUAN Yun-sheng, et al %T Transcription factor interactions of TGF-beta signaling pathway identified by yeast two-hybrid technique during mice liver regeneration %0 Journal Article %D 2011 %J Journal of Shanghai Jiao Tong University (Medical Science) %R 10.3969/j.issn.1674-8115.2011.05.006 %P 551- %V 31 %N 5 %U {https://xuebao.shsmu.edu.cn/CN/abstract/article_9079.shtml} %8 2011-05-28 %X

Objective To investigate the interactions of four transcription factors of transforming growth factor-β (TGF-β) signaling pathways using yeast two-hybrid technique. Methods CCl4-induced liver injury mouse models were established, and the total RNA of liver tissues were extracted as the template for cDNA by RT-PCR. Activating transcription factor 3 (ATF3), inhibitor of DNA binding 3 (ID3), DNA-damage-inducible transcript 3 (DDIT3) and LIM domains 2 (FHL2), which were differentially expressed detected by liver regeneration gene chip and associated with TGF-β signaling pathway, were served as candidate genes. After gene clone, vector construction and PCR and DNA sequencing, yeast two-hybrid system was used to indentify the interactions among ATF3, ID3, DDIT3 and FHL2 with auto-activation test. Results PCR and DNA sequencing indicated that ATF3, FHL2, DDIT3 and ID3 were successfully cloned, and there was no auto-activation phenomenon after auto-activation test. Seven protein interactions (ID3/ATF3, FHL2/ATF3, ATF3/ATF3, FHL2/FHL2, ID3/FHL2, ID3/ID3 and ID3/DDIT3) were identified by yeast two-hybrid technique. Conclusion Protein interactions among ATF3, FHL2 and ID3 may be one of the possible ways to regulate the TGF-β signaling pathway, and protein-protein interactions may be a key gateway to liver regeneration.