%A WANG Fang-yuan, XIE Chao, CHENG Wei-wei, et al %T Establishment of mouse model of cyclophosphamide-induced ovarian failure %0 Journal Article %D 2011 %J Journal of Shanghai Jiao Tong University (Medical Science) %R 10.3969/j.issn.1674-8115.2011.10.009 %P 1398- %V 31 %N 10 %U {https://xuebao.shsmu.edu.cn/CN/abstract/article_9274.shtml} %8 2011-10-28 %X

Objective To establish the mouse model of chemotherapy-induced ovarian failure. Methods Female C57BL/6 mice were intraperitoneally injected with cyclophosphamide at the dose of 75 mg/kg. The general conditions, estrous cycles, serum estrogen (estradiol) levels, morphology of ovarian tissues, changes of numbers of follicles, secretion of anti-Mullerian hormone and tissue fibrosis of mice were observed. Mice without cyclophosphamide injection were served as controls. Results Compared with controls, the serum estrogen level and secretion of anti-Mullerian hormone of ovarian tissues decreased, there were significant stromal hyperplasia and fibrosis in ovarian tissues, and the number of follicles reduced in mice injected with cyclophosphamide. Conclusion Intraperitoneal injection of 75 mg/kg cyclophosphamide may cause obvious ovarian failure, and the changes in reproductive endocrine and ovarian histopathology are similar to those of chemotherapy-induced ovarian failure in female patients in clinics. It can be used to establish the animal model of chemotherapy-induced ovarian failure due to its simplicity, feasibility, high success rate and low mortality.