%A LIANG Yong-jun, ZHOU Ci-xiang, YU Xiao-ting, et al %T miR-506 targets Slug to regulate epithelial-to-mesenchymal transition and metastasis of breast cancer %0 Journal Article %D 2012 %J Journal of Shanghai Jiao Tong University (Medical Science) %R 10.3969/j.issn.1674-8115.2012.06.003 %P 699- %V 32 %N 6 %U {https://xuebao.shsmu.edu.cn/CN/abstract/article_9591.shtml} %8 2012-06-28 %X

Objective To investigate the role of miR-506 targeting Slug in regulation of epithelial-to-mesenchymal transition and metastasis of breast cancer. Methods The expression of miR-506 in eight breast cancer cell lines (MCF7, BT474, SK-BR-3, MDA-MB-453, MDA-MB-468, HCC1937, BT549 and MDA-MB-231) was determined by Real-Time fluorescence quantitative PCR, and human normal mammary epithelial cell MCF10A was served as control. miR-506 mimics were transiently overexpressed in MDA-MB-231 and BT549 cells through liposome transfection(negative control). The mobility of cells was detected by wound healing assay and Transwell migration and invasion assay. The morphological change of cells after transfection with miR-506 was observed, and the expression of E-cadherin, Vimentin and Slug protein was determined by Western blotting. The effect of miR-506 overexpression in MDA-MB-231 cells on wild type luciferase was determined by luciferase reporter assay. Results The expression of miR-506 in breast cancer cells was significantly lower than that in MCF10A cells (P<0.01). The overexpression of miR-506 significantly inhibited the migration and invasion ability of MDA-MB-231 and BT549 cells, which was significantly different from that of negative control (P<0.01). Obvious morphological change was observed in MDA-MB-231 and BT549 cells 48 h after transient transfection with miR-506. Western blotting indicated that miR-506 overexpression increased the expression of E-cadherin and decreased the expression of Vimentin in MDA-MB-231 and BT549 cells. Western blotting revealed that the expression of Slug protein in MDA-MB-231 cells after transfection with miR-506 was significantly lower than that in negative control, and luciferase reporter assay demonstrated that miR-506 overexpression significantly inhibited the activity of wild type luciferase. Conclusion MiR-506 targets Slug to regulate epithelial-to-mesenchymal transition and inhibit metastasis of breast cancer, which may be one of the ways for miR-506 to participate in the development of breast cancer.