Most Down Articles

    Published in last 1 year | In last 2 years| In last 3 years| All| Most Downloaded in Recent Month| Most Downloaded in Recent Year|

    Most Downloaded in Recent Month
    Please wait a minute...
    For Selected: Toggle Thumbnails
    Research progress of effect of carotenoids on maternal and child health
    WU Ke1, SUN Han-xiao2, CAI Mei-qin1
       2019, 39 (8): 929-.   DOI: 10.3969/j.issn.1674-8115.2019.08.021
    Abstract409)      PDF(pc) (7354KB)(1987)       Save
    Early life nutrition plays an important role in determining the pregnancy outcomes and offspring lifelong health. Carotenoids deficiency is associated with adverse pregnancy outcomes such as preeclampsia, premature delivery and intrauterine growth restriction. Carotenoids possess antioxidant, inflammation modulating and immune-enhancing properties and promote visual, cognitive and respiratory health in offsprings. Among carotenoids, α-carotene, β-carotene and β-cryptoxanthin can be transformed into vitamin A in vivo, and their conversion rates are affectedthe nutritional status of vitamin A. Lutein and zeaxanthin are highly enriched in the brain and retina of infants and young children, which are closely related to the development of visual acuity and cognitive function. Breast milk contains an adequate level of lutein and its absorption rate is significantly higher than that of infant formulas. Consequently lutein supplementation is necessary for artificially fed infants, especially premature infants. In this paper, the functional research progresses of carotenoids related to adverse pregnancy outcomes and offspring development, as well as the present situation of carotenoids supplementation in formula were reviewed.
    Related Articles | Metrics
    Inhibitory effect of sanguinarine on proliferaton and invasion of gastric cancer cells by upregulating m 6A methyltransferase 14
    Ming CHEN, Jing ZHANG
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 135-141.   DOI: 10.3969/j.issn.1674-8115.2022.02.001
    Abstract113)   HTML365)    PDF(pc) (2415KB)(238)       Save
    Objective

    ·To investigate the effect of sanguinarine (SAG) on the proliferation and invasion of gastric cancer cells MGC-803 and AGS, and the relationship between the mechanism and N6-methyladenosine (m6A) methyltransferase 14 (METTL14).

    Methods

    ·After the gastric cancer cell lines (MGC-803 and AGS) were exposed to different concentrations of SAG (0, 10, 20 μmol/L) for 48 h, quantitative PCR and Western blotting analysis were used to detect the effect of SAG on the expression of METTL14. Then, MGC-803 and AGS cells transfected with lentiviruses-mediated small interfering RNA of METTL14 (si-METTL14) or control (si-NC) were treated with 10 μmol/L SAG or PBS for 48 h, and thus the two cell lines were divided into si-METTL14+SAG group, si-NC+SAG group and si-NC+PBS group, respectively. Quantitative PCR and Western blotting were used to verify the expression levels of METTL14 after it was transfected with si-METTL14 in gastric cancer cells. The proliferation level, number of clones formed and invasion potential of the 3 groups in both MGC-803 cells and AGS cells were observed by MTT proliferation assay, cell clone formation test and Transwell invasion assay, respectively. Independent samples t test was used for comparison between two groups of data, and one-way ANOVA was used for comparison between more than two groups of data.

    Results

    ·Compared with the control group (0 μmol/L), 10 μmol/L SAG and 20 μmol/L SAG up-regulated METTL14 mRNA and protein expression levels (P<0.05), showing a certain concentration dependence. Quantitative PCR and Western blotting results confirmed that the expression levels of METTL14 in gastric cancer cells were significantly reduced after transfection of si-METTL14 in both MGC-803 cells and AGS cells. MTT cell proliferation assay showed that the cell proliferation rate of the si-NC+SAG group was significantly lower than that of the si-NC+PBS group in each cell line (P=0.000). The cell clone formation test showed that the number of cell clones of the si-NC+SAG group was significantly smaller than that of the si-NC+PBS group in each cell line (P<0.01). The Transwell invasion assay showed that the cells crossing Matrigel gel in the si-NC+SAG group was significantly less than that in the si-NC+PBS group in each cell line (P<0.01). si-METTL14 could partially reverse the inhibitory effects of SAG on gastric cancer cells.

    Conclusion

    ·SAG can inhibit the proliferation activity, clonal formation and invasion potential of gastric cancer cells, which may be realized by upregulating METTL14 expression level.

    Table and Figures | Reference | Related Articles | Metrics
       2020, 40 (3): 2-.  
    Abstract269)      PDF(pc) (10722KB)(430)       Save
    Related Articles | Metrics
    Interventional effects of nicotinamide mononucleotide on metabolism in aging mice
    Guodong DANG, Xinyu HONG, Meiqin CAI
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 158-165.   DOI: 10.3969/j.issn.1674-8115.2022.02.004
    Abstract98)   HTML28)    PDF(pc) (1837KB)(186)       Save
    Objective

    ·To investigate the effects of nicotinamide mononucleotide (NMN) on metabolism in aging mice.

    Methods

    ·Seventy C57BL/6N male mice were randomly divided into 5 groups by using a table of random numbers. They were the control group, the premature aging model group, the aging model group, the intervention group Ⅰand the intervention group Ⅱ. Each group contained 14 mice. Except the control group, D-galactose (D-gal) (150 mg/kg) was subcutaneously injected into the napes of mice in the other 4 groups to establish the aging model of mice. NMN (300 mg/kg) was given to the intervention group Ⅰ and the intervention group Ⅱ by intragastric administration at the same time, and the other groups were given the same amount of distilled water, once a day, for 6 weeks in the premature aging model group and the intervention group Ⅰ, and for 12 weeks in the aging model group and the intervention group Ⅱ. The control group was given the same amount of normal saline and distilled water, once a day, for 6 weeks. Six weeks after modeling, the energy metabolism levels of the mice in the control group, the premature aging model group and the intervention group Ⅰ were detected, including respiratory metabolism, activity level and energy consumption. The organ indexes of thymus, spleen, liver and kidney were calculated. The glucose tolerance and insulin sensitivity were measured. In addition, the content of superoxide dismutase (SOD) and the activities of glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the serum and liver tissue were detected. Twelve weeks after modeling, the above indexes were detected in the aging model group and the intervention group Ⅱ.

    Results

    ·Compared with the control group, the thymus index (P=0.035, P=0.000) and renal index (P=0.009, P=0.002) of the model groups were significantly decreased. The O2 consumption (P=0.018, P=0.000), CO2 exhalation (P=0.044, P=0.003), energy consumption (P=0.010, P=0.001) and activity ability (both P=0.000) of the premature aging model group and the aging model group were significantly decreased at night. The insulin sensitivity was significantly reduced (P=0.012, P=0.011). The activities of SOD (P=0.002, P=0.001) and GSH-Px (P=0.001, P=0.011) in serum were significantly decreased and the content of MDA in serum was significantly increased (both P=0.000). The decline of energy metabolism levels, thymus and kidney indexes and antioxidant index verified the success of D-gal aging model. Compared with the premature aging model group, the intervention group Ⅰ had no significant difference in respiratory metabolism, energy consumption, glucose tolerance, insulin sensitivity and other indicators (all P>0.05). But in the intervention group Ⅰ, the activity ability was significantly improved (P=0.022), the activities of SOD (P=0.026) and GSH-Px (P=0.006) in serum were significantly increased, and the MDA content in serum was significantly decreased (P=0.011). Compared with the aging model group, the O2 consumption (P=0.045), CO2 exhalation (P=0.030), activity ability (P=0.049) and energy consumption (P=0.043) in the intervention group Ⅱ were significantly increased at night. Compared with the aging model group, the impaired glucose tolerance was improved (P=0.030), the insulin sensitivity was increased (P=0.010)in the intervention group Ⅱ, the activity of SOD in serum was significantly increased (P=0.046), and the MDA content in serum and liver tissue was significantly decreased (P=0.000). There was no significant difference in the activity of GSH-Px in serum and liver tissue between the two groups (P>0.05).

    Conclusion

    ·NMN can improve the metabolic level of aging mice to a certain extent, and its mechanism may be related to improving the antioxidant capacity of the body.

    Table and Figures | Reference | Related Articles | Metrics
    Establishment of a nomogram clinical scoring system for the risk of heterotopic ossification in patients undergoing surgery after fracture
    Yekai WANG, Wei CHEN, Yinghui YANG, Jingze WU, Heping WANG, Yanzhen YAO, Zhoujun BAO
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 166-172.   DOI: 10.3969/j.issn.1674-8115.2022.02.005
    Abstract69)   HTML33)    PDF(pc) (1837KB)(168)       Save
    Objective

    ·To establish a nomogram scoring system to predict morbidity of heterotopic ossification (HO) in the patients undergoing surgery after fracture.

    Methods

    ·From August 2018 to October 2020, 124 patients with fractures from department of orthopaedics or brain surgery, including 81 males and 43 females with an average age of (56.48±15.45) years old (ranging from 25?91 years old), were enrolled in the study. Clinical features including gender, age, with brain trauma or not, anesthetic mode, operation duration time, and blood loss during operation were collected. Peripheral blood samples were collected at three time points, i.e. 1 d, 7 d and 15 d after fracture, and the sera levels of bone morphogenetic protein 2 (BMP-2), BMP-4, BMP-5, BMP-7, interleukin 4 (IL-4), IL-6, IL-10, interferon γ (IFN-γ), and transforming growth factor-β (TGF-β) were detected by ELISA. All the patients were divided into HO group and non-HO group according to follow-up outcomes. The variables with statistically significant differences (α=0.15) between the two groups selected from clinical and laboratory indications were included in the univariate Cox regression model. Then the variables selected from univariate Cox regression model (α=0.05) were incorporated into multivariate Cox regression model to screen the independent risk factors of HO and calculate the risk ratios (HR). Finally, the nomogram scoring system was output through rms package in R language.

    Results

    ·The follow-up observation found 13 HO cases after a median time of 71 (38, 292) d. Seven independent risk factors for HO, i.e. brain trauma (HR=2.932, P=0.038), operation duration time (HR=1.005, P=0.007), blood loss during operation (HR=1.004, P=0.022), BMP-2 (15d) (HR=1.009, P=0.044), BMP-4 (15 d) (HR=1.004, P=0.011), TGF-β (15 d) (HR=1.011, P=0.046), and BMP-7 (7 d) (HR=1.004, P=0.008), were selected by comparison between groups, univariate COX regression and multivariate COX regression sequentially. The nomogram to predict morbidity of HO after 90 d, 180 d, and 360 d was generated.

    Conclusion

    ·The seven scoring indexes of nomogram scoring system for predicting the risk of HO in the patients undergoing surgery after fracture are with brain trauma or not, operation duration time, blood loss during operation, levels of BMP-2, BMP-4 and TGF-β in peripheral blood 15 d after fracture and BMP-7 7 d after fracture.

    Table and Figures | Reference | Related Articles | Metrics
    Effects of Pcsk9 gene interference on high fat-induced nonalcoholic fatty liver disease with atherosclerosis in rats
    Xiaowen ZHANG, Yi WANG, Chan ZHANG, Di ZHANG, Hang YUN, Di HUANG
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 150-157.   DOI: 10.3969/j.issn.1674-8115.2022.02.003
    Abstract84)   HTML239)    PDF(pc) (2405KB)(184)       Save
    Objective

    ·To investigate the effects of proprotein convertase subtilisin kexin 9 (Pcsk9) gene knockdown on non-alcoholic fatty liver disease (NAFLD) and atherosclerotic lesions in rats induced by high fat.

    Methods

    ·The SD rat model of NAFLD was established. Rats were randomly divided into 4 groups: control group, model group (high fat), shRNA-negative control (NC) interference model group (high fat+shRNA-NC) and Pcsk9-shRNA interference model group (high fat+ Pcsk9-shRNA). Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect Pcsk9 gene interference efficiency. Fasting serum insulin was determined by radioimmunoassay. Automatic biochemical analyzer was used to detect levels of blood lipid in rats. Hematoxylin-eosin staining (H-E staining) was used to observe the injury of liver tissue and aorta tissue. Apoptosis of liver tissue was detected by TUNEL staining. The levels of interleukin-1β (IL-1β), IL-6 and inducible nitric oxide synthase (iNOS) in peripheral blood were detected by enzyme linked immunosorbent assay (ELISA). The expression of PCSK9, Toll-like receptor 4 (TLR4), nuclear factor κB (NF-κB) P65 and tumor necrosis factor α (TNF-α) were detected by Western blotting.

    Results

    ·Compared with the control group, obesity index and insulin level in the model group were significantly increased (all P=0.000); the apoptosis rate of liver cells was significantly increased (P=0.000); the level of high density lipoprotein cholesterol (HDL-C) was significantly decreased, while the levels of low density lipoprotein cholesterol (LDL-C), total cholesterol (TC) and triacylglycerol (TAG) were significantly increased (all P=0.000); the levels of IL-1β, IL-6 and iNOS were significantly increased (all P=0.000); TLR4, NF-κB P65 protein activation and TNF-α expression were significantly increased (all P=0.000); the liver tissue and aorta tissue were significantly damaged. After interference of Pcsk9 gene expression, compared with the model group, obesity index and insulin level in the high fat+Pcsk9-shRNA group were significantly reduced (P=0.007, P=0.000); the apoptosis rate of liver cells was significantly reduced (P=0.000); the level of HDL-C was significantly increased while the levels of LDL-C, TC and TAG were significantly decreased (all P=0.000); the levels of IL-1β, IL-6 and iNOS were significantly decreased (all P=0.000); TLR4, NF-κB P65 protein activation and TNF-α expression were significantly decreased (all P=0.000); the histopathological lesions of liver tissue and aorta tissue were improved.

    Conclusion

    ·Knockdown of Pcsk9 gene can reduce obesity index, insulin level, blood lipid index and inflammatory response in the rats with NAFLD and atherosclerosis, and inhibit the activation of TLR4 and NF-κB P65 protein, thereby improving the injury of liver and aortic tissue in rats.

    Table and Figures | Reference | Related Articles | Metrics
    Application of three-dimensional electron microscopy to morphological study of neurons in brainstem cochlear nucleus
    Jialei ZHOU, Haibin SHENG, Haoyu WANG, Yan LU, Fangfang WANG, Hao WU, Yunfeng HUA
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 142-149.   DOI: 10.3969/j.issn.1674-8115.2022.02.002
    Abstract73)   HTML356)    PDF(pc) (3073KB)(172)       Save
    Objective

    ·To explore the feasibility of cross-scale neuroanatomy and connectomics of mouse cochlear nucleus (CN) by using a new three-dimensional (3D) electron microscopic imaging method.

    Methods

    ·The intact CN was obtained after the brain tissue of adult CBA/Ca mice (2 months old) was fixed and dissected, stained with heavy metals (reducing osmium amplification method) , dehydrated with gradient ethanol and anhydrous acetone and embedded with low viscosity resin. The CN tissue was imaged by X-ray microscopy and 3D reconstruction, and its staining quality was evaluated. Then targeted subdivision was located according to the specific distribution of auditory nerve fibers. The low-resolution pre-scan of the CN tissue was performed by scanning electron microscopy, and compared with X-ray microscopic dataset. After the target location was determined, the volume of interest was mapped by serial block-face scanning electron microscopy (SBEM) for 3D reconstruction, and the ultrastructures of bushy cells and the synapses projecting on its surface were tracked, annotated and reconstructed.

    Results

    ·3D electron microscopic samples of intact CN of CBA/Ca mice were successfully prepared. The 3D structure of CN with cell resolution was collected and reconstructed by X-ray microscopic imaging, and the anatomical localization of subregions of CN was achieved. The 3D electron microscopic data of bushy cells in the target area of CN were successfully collected by SBEM. The tracking, labeling and reconstruction of endbulb of Held synapses and other non-auditory synapses on the cell body of target bushy cells were completed. The data of 3D electron microscopy showed that there were 5 endbulb of Held synapses projecting to the surface of the target bushy cell, forming a total of 348 synaptic active zones, while there were 97 synapses from non-auditory nerve.

    Conclusion

    ·It is feasible to prepare 3D electron microscopic samples of adult mouse intact CN by Osmium-based enbloc staining and resin embedding. X-ray microscopic imaging can be used for rapid and accurate localization of subregions and target volume of CN, and the acquired 3D electron microscopic data can be used to study the neuronal morphology and synaptic connections in the CN.

    Table and Figures | Reference | Related Articles | Metrics
    Spatial-temporal distribution of coronavirus disease 2019 in Jingzhou City
    LIN Mao-wen*, LIU Tian*, TIAN Ke-qing, JIANG Hong, ZENG Min-min, WANG Li, YIN Jun, LEI Ruo-qian, YAO Meng-lei, HUANG Ji-gui
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (05): 566-572.   DOI: 10.3969/j.issn.1674-8115.2020.05.002
    Abstract503)      PDF(pc) (7202KB)(356)       Save
    Objective · To explore the spatial distribution and spatial-temporal clustering of coronavirus disease 2019 (COVID-19) in Jingzhou City. Methods · Data of COVID-19 cases in Jingzhou City from January 1 to March 12, 2020 were collected. Trend surface analysis, spatial autocorrelation and spatial-temporal scanning analysis were conducted to understand the spatial-temporal distribution of COVID-19 at town (street) level in Jingzhou City, and the spatial-temporal clustering characteristics of local cases and imported cases were compared. Results · Trend surface analysis showed that the incidence rate of COVID-19 in Jingzhou City was slightly “U” from west to east, slightly higher in the east, and inverted “U” from south to north, slightly higher in the south. Global autocorrelation showed that the incidence rate of COVID-19 in Jingzhou City was positively correlated (Moran's I=0.410, P=0.000). Local spatial autocorrelation analysis showed that the highly clustered areas and hot spot areas were mainly in Shashi District, Jingzhou District and the main urban area of Honghu City (Xindi Street) (P<0.05). Five clusters were found by spatial-temporal scanning of imported cases. The cluster time of the main cluster was from January 18 to February 3, 2020, and it was centered on Lianhe Street, covering 15 towns (streets) in Shashi District and Jingzhou District (LLR=174.944, RR=7.395, P=0.000). Five clusters were found by spatial-temporal scanning of local cases. The cluster time of the main cluster was from January 20 to February 24, 2020, which was located in Xindi Street, Honghu City (LLR=224.434, RR=16.133, P=0.000). Conclusion · Obvious spatial-temporal clustering of COVID-19 was found in Jingzhou City, and Shashi District, Jingzhou District and Honghu City were the most prevalent areas.
    Related Articles | Metrics
    Correlation between childhood trauma and schizophrenia symptoms
    REN Yan-yan, LÜ Qin-yu, YANG Wei-li, WANG Zhen
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (12): 1641-1645.   DOI: 10.3969/j.issn.1674-8115.2020.12.013
    Abstract220)      PDF(pc) (6330KB)(246)       Save
    Objective · To explore the relationship between different dimensions of childhood trauma and positive and negative symptoms of schizophrenia. Methods · A total of 124 patients with schizophrenia were recruited at Shanghai Mental Health Center. Childhood trauma was evaluated by the Early Trauma Inventory Short Form (ETI-SF), and psychotic symptoms were evaluated by the Positive and Negative Syndrome Scale (PANSS). Pearson correlation analysis and Gaussian graphical model (GGM) were used to examine the relationship between 4 dimensions of ETI-SF and the scores of 3 subscales or total 30 items of PANSS. Results · There was a significant correlation between subscales of positive symptoms and physical abuse (r=0.29, P=0.000) or emotional abuse (r=0.21, P=0.024) after controlling age, sex and other confounding factors. All dimensions of ETI-SF were related to different items of positive symptom scale. GGM results also verified that physical and emotional abuse were strongly correlated with the positive symptoms in schizophrenia. In addition, the degree centrality value of active social avoidance was the biggest through the whole network. Conclusion · Childhood trauma is related to positive symptoms of schizophrenia. Physical abuse and emotional abuse are significantly related to the positive symptoms. Active social avoidance plays an important role in the whole network.
    Related Articles | Metrics
    Analysis of clinicopathologic features and prognosis of eight children with granulosa cell tumor of ovary
    Zhengwen XING, Ying WU, Xueli WANG, Qingyu WANG, Wenting WANG, Zhi LI, Bin ZHANG, Jing JIN
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 192-196.   DOI: 10.3969/j.issn.1674-8115.2022.02.009
    Abstract79)   HTML31)    PDF(pc) (3077KB)(188)       Save
    Objective

    ·To investigate the clinicopathological features and prognosis of ovarian granulosa cell tumor (GCT) in children.

    Methods

    ·The clinicopathologic and follow-up data of 8 patients with ovarian GCT in Shanghai Children's Hospital from June 2008 to June 2018 were collected and retrospectively studied to summarize the clinicopathological features and analyze the prognosis.

    Results

    ·The age of onset in this group ranged from 2 to 12 years with median age of 6.5 years. Two cases were adult granulosa cell tumor (AGCT) and six cases were juvenile granulosa cell tumor (JGCT). The first symptoms were abdominal pain or abdominal mass. Six patients were complicated with symptoms of precocious puberty, including 5 cases of true precocious puberty and 1 case of pseudo precocious puberty. The levels of peripheral blood sex hormones changed to varying degrees. Gross examination showed that the tumor masses ranged from 4 to 22 cm in the greatest dimension (average 12.8 cm). All of the 8 tumors were mixed solid-cystic in appearance. Under light microscope, AGCT showed obvious nuclear sulcus and microfollicular structure, containing eosinophilic substances, namely characteristic Call-Exner bodies. JGCT had follicular structures of different sizes, containing basophilic secretions. The inner layer of the follicular wall was composed of granular cells, which could be surrounded by follicular membrane cells. No Call-Exner bodies were found, and nuclear sulcus were rare. The results of immunohistochemistry showed that α-inhibin and CD99 were positive, and epithelial membrane antigen (EMA) were negative in all the cases. In 5 cases calretinin were positive, and in 5 cases cytokeratin (CK) were positive. Ki-67 proliferation index was 5%?50%. There were 3 estrogen receptor (ER) positive cases and 6 progesterone receptor (PR) positive cases. Forkhead transcription factor 2 (FOXL2) was highly expressed in 2 cases of AGCT and 6 cases of JGCT. SRY-box transcription factor 9 (SOX9) positive cells were scattered in 2 cases of AGCT and 3 cases of JGCT. All the cases were treated with standard surgery. Tumor rupture or metastasis was found in 5 cases, and ascites was suspiciously positive in 1 case. Eight children with ovarian GCT were followed up for 19?155 months, and all of them survived in good health.

    Conclusion

    ·The clinical features of AGCT and JGCT in children's ovaries are similar and have typical histopathological features respectively. The selection of suitable immunohistochemical detection method is helpful for differential diagnosis of ovarian tumors in children. The prognosis of ovarian GCT in children is good even with tumor rupture after radical surgery.

    Table and Figures | Reference | Related Articles | Metrics
    Research progress of nursing ethics in liver transplantation
    LU Ye-feng, ZHAO Ai-ping, WANG Yan
       2011, 31 (6): 723-.   DOI: 10.3969/j.issn.1674-8115.2011.06.009
    Abstract1555)      PDF(pc) (4238KB)(1617)       Save

    Liver transplantation brings not only hopes to patients with end-stage hepatic diseases, but also many ethical problems to society. The research status of nursing ethics in liver transplantation is reviewed in this paper, in hopes of offering some ideas to nurses working for liver transplantation.

    Related Articles | Metrics
    Screening potential hub genes associated with myocardial ischemia-reperfusion injury in mice based on GEO database and bioinformatics analysis
    Jianru WANG, Guangcao PENG, Mingjun ZHU
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (1): 51-62.   DOI: 10.3969/j.issn.1674-8115.2022.01.008
    Abstract104)   HTML13)    PDF(pc) (8936KB)(157)       Save
    Objective

    ·To screen the potential hub genes associated with myocardial ischemia-reperfusion injury (MIRI) in mice by bioinformatics analysis based on gene expression omnibus (GEO) database.

    Methods

    ·The mouse MIRI data sets GSE61592, GSE83472 and GSE160516 were obtained from GEO database. The differentially expressed genes (DEGs) in each data set were screened by limma package, and then robust DEGs were screened by robust sorting integration (RRA) method. The protein-protein interaction (PPI) network of robust DEGs was constructed, and the submodules and hub genes in the PPI network were screened. The clusterProfiler package was used to analyze the robust DEGs, the most important submodule genes and hub genes by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Eighteen male C57BL/6 J mice aged 6?8 weeks were randomly divided into sham group and MIRI group, 9 mice each group. The MIRI model was constructed by left anterior descending coronary artery ligation for 30 min ischemia and 24 h reperfusion, and the mRNA expression of hub genes was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

    Results

    ·RRA method identified 294 robust DEGs in three data sets. In PPI network, a total of 14 sub-modules were screened, of which module 1 was the most important and 17 hub genes were found. GO and KEGG analysis showed that the robust DEGs, module 1 genes, and the hub genes were mainly involved in regulating the migration of inflammatory cells, the activity of chemokines and cytokines and their receptors, Toll-like receptors and other biological function and signaling pathways. RT-qPCR results showed that compared with the sham group, the expressions of chemokine (C-C motif) ligand 4 (Ccl4), Ccl6, Ccl7, chemokine (C-X-C motif) receptor 4 (Cxcr4), chemokine (C-C motif) receptor 2 (Ccr2), signal-regulatory protein β1 (Sirpb1), low affinity immunoglobulin gamma Fc region receptor Ⅱb (Fcgr2b), leukocyte surface antigen CD53 (Cd53), arachidonate 5-lipoxygenase activating protein (Alox5ap), myeloid differentiation primary response gene 88 (Myd88), macrophage scavenger receptor 1 (Msr1), matrix metallopeptidase 14 (Mmp14), triggering receptor expressed on myeloid cells 2 (Trem2) and leupaxin (Lpxn) were up-regulated in the myocardium of the MIRI group, but there was no difference in low affinity immunoglobulin gamma Fc region receptor Ⅲ (Fcgr3), complement C1q subcomponent subunit B (C1qb) and a disintegrin and metalloproteinase domain-containing protein 8 (Adam8). By reviewing the literatures, Trem2, Lpxn, Cd53, Alox5ap, Sirpb1 and Fcgr2b were not reported to participate in MIRI.

    Conclusion

    ·This study has unearthed 6 potential hub genes for MIRI in mice, and the results can provide new ideas and entry points for further exploring the molecular mechanism and therapeutic targets of MIRI.

    Table and Figures | Reference | Related Articles | Metrics
    Research progress of effect and mechanism of protein arginine methyltransferase in tumors
    WEN Jun, MIN Xue-jie, ZHAO Li, ZHAO Xiao-ping
       2017, 37 (6): 842-.   DOI: 10.3969/j.issn.1674-8115.2017.06.022
    Abstract1211)      PDF(pc) (7473KB)(1918)       Save

     Protein arginine methyltransferase (PRMT) can methylate a variety of proteins, including histones and non-histones, affecting a variety of cellular processes such as transcription, RNA splicing, DNA repair, and cell cycle control. The activity of PRMT is affected by a variety of regulatory mechanisms, and its abnormal expression plays an important role in the development of the diseases, especially in tumors such as breast cancer and leukemia. PRMT has a unique value in the diagnosis or treatment of tumors. With the further exploration of PRMT methylation mechanism, PRMT selective inhibitors have made some progress. PRMT-specific inhibitors are expected to be targeted drugs for the treatment of tumors.

    Related Articles | Metrics
    Comparison of mismatch negativity in patients with schizophrenia and depression
    Meng-ting SHEN, Xuan-hong ZHANG, Zhen-ying QIAN, Hui LI, Jian-hua SHENG, Ji-jun WANG, Ying-ying TANG
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2021, 41 (8): 1041-1045.   DOI: 10.3969/j.issn.1674-8115.2021.08.007
    Abstract132)   HTML9)    PDF(pc) (1250KB)(167)       Save
    Objective

    ·To compare the difference of mismatch negativity (MMN) between first-episode schizophrenia and depression and its correlation with clinical characteristics, and explore the role of pre-attention processing in the mechanisms of schizophrenia and depression.

    Methods

    ·Twenty patients with schizophrenia and 19 patients with depression were selected in the outpatient department of Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine from January 2014 to December 2016. Healthy controls were also recruited. The Positive and Negative Syndrome Scale (PANSS) was used to measure the clinical symptoms of schizophrenia. Hamilton Depression Scale (HAMD) and Hamilton Anxiety Scale (HAMA) were used to measure the clinical symptoms of depression. All patients and healthy controls completed MMN detection and clinical scale assessments. The amplitudes and latencies of duration MMN (DMMN) and frequency MMN (FMMN) were analyzed by repeated measures analysis of variance with group as the between-group factors. The midline electrodes (Fz, Fcz) were used as the with-in group factor; meanwhile, region (F, Fc) and laterality (1 for left, 2 for right) were used as the within-group factors for lateral electrodes and the factor sex was used as covariate. Partial correlations was performed to find the correlation between MMN and clinical characteristics.

    Results

    ·① The amplitude of DMMN in patients with schizophrenia 2 for [(-2.70±2.46) μV)] was lower than that in patients with depression [(-5.06±0.46) μV] and healthy controls [(-5.15±0.43) μV] (both P≤0.001). No significant group differences of DMMN latency were observed at midline or lateral electrodes (All P>0.05). ② There was no significant group difference of FMMN amplitudes at midline electrodes (P>0.05) but a significant between-group difference at lateral electrodes (P=0.040). No significant group differences of FMMN latency were observed at midline or lateral electrodes (All P>0.05). ③ There was a significant correlation between DMMN amplitudes at F2 (P=0.042) or Fz (P=0.032) and general scores of PANSS in schizophrenia patients. There was no correlation in depression patients.

    Conclusion

    ·Abnormality of DMMN exists in the patients with first episode schizophrenia but not in the patients with depression, suggesting that DMMN amplitudes may work as a biological marker to distinguish schizophrenia and depression.

    Table and Figures | Reference | Related Articles | Metrics
    Meta-analysis of impacts of inhaled nitric oxide on postoperative renal injury and hemorrhage
    Dewei QIAN, Ren ZHOU, Lichun GUAN, Hang ZHANG, Min YU
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (1): 95-100.   DOI: 10.3969/j.issn.1674-8115.2022.01.014
    Abstract66)   HTML4)    PDF(pc) (1964KB)(142)       Save
    Objective

    ·To evaluate the effects of inhaled nitric oxide on renal injury and hemorrhage after surgery.

    Methods

    ·China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (CBM), WanFang Data, PubMed, Embase, Web of Science and The Cochrane Library were searched systematically from the establishment of each database to March 26, 2021. “外科手术” and “一氧化氮” were used as Chinese keywords for retrieval. Correspondingly, the “nitric oxide”“surgical procedures, operative”“cardiac surgical procedures”“cardiac surgery” and “transplantation” were used as subject words and free words for searching. The literatures were screened according to the pre-established inclusion and exclusion criteria. Meta-analysis was performed by using RevMan 5.3. The study end points were the incidences of renal injury and hemorrhage.

    Results

    ·Seven literatures were included for analysis, with a total of 689 surgical patients. Of them, 337 received nitric oxide treatment and 352 received placebo treatment. Compared with the control group, patients treated with nitric oxide demonstrated a statistically difference in reducing the incidence of postoperative renal injury (RR= 0.75, 95%CI 0.61?0.92, P=0.005). There was no significant difference in the incidence of hemorrhage (RR=0.79, 95%CI 0.22?2.83, P=0.710) between the two groups.

    Conclusion

    ·Application of nitric oxide after surgery does not increase the risks of renal injury and hemorrhage. In contrast, the use of nitric oxide in cardiopulmonary bypass might protect renal function.

    Table and Figures | Reference | Related Articles | Metrics
    Summary of the best evidence summary on prevention strategies for diaper dermatitis in infants
    Yan LI, Yan JIANG, Qiongfang KANG, Qunfeng LU
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (3): 357-363.   DOI: 10.3969/j.issn.1674-8115.2022.03.014
    Abstract14)      PDF(pc) (1118KB)(112)       Save
    Objective

    ·To search, appraise and retrieve the best evidence on prevention strategies for diaper dermatitis in infants, and provide evidence-based guidance for reducing the diaper dermatitis.

    Methods

    ·BMJ Best Practice, UpToDate, National Institute for Health and Care Excellence, Scottish Intercollegiate Guidelines Network, Registered Nurses' Association of Ontario, Joanna Briggs Institute (JBI) Center of Evidence-Based Healthcare Database, Database of Abstracts of Reviews of Effectiveness, Cochrane Library, Campbell, Medline, PubMed, SinoMed, Chinese Nursing Association, and Chinese Pediatric Society, Chinese Medical Association were systematacially searched to collect relevant evidence on preventing diaper dermatitis in infants. The evidence including clinical decisions, guidelines, evidence summaries, systematic reviews and expert consensuses published from database construction to June 2021. Two researchers evaluated the quality of the literature, and extracted recommendations and research conclusions from the included literature.

    Results

    ·Totally 8 articles were involved, including 2 clinical decisions, 1 evidence summary, 4 systematic reviews and 1 expert consensus. Finally, 26 pieces of best evidence about air, barrier, cleaning, diaper and education were summarized.

    Conclusion

    ·Pediatric professionals should optimize the clinical process to prevent diaper dermatitis, which is on the basis of evidence, cultural characteristics, resource allocation and patient characteristics. Family education is also important, aiming at reducing the incidence rate of diaper dermatitis in infants.

    Table and Figures | Reference | Related Articles | Metrics
    Effects of computerized cognitive correction therapy on cognitive function of schizophrenia
    Guo-qin HU, Cheng-qing YANG, Qin-yu LÜ, Jing ZHAO, Ming-huan ZHU, Zheng-hui YI, Xin-hai DAI
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2021, 41 (5): 622-627.   DOI: 10.3969/j.issn.1674-8115.2021.05.010
    Abstract170)   HTML10)    PDF(pc) (1204KB)(143)       Save
    Objective

    ·To explore the effects of computerized cognitive correction therapy (CCRT) on the cognitive function of patients with chronic schizophrenia and the effects on plasma brain-derived nerve growth factor (BDNF) and tyrosine kinase receptors B (TrK-B) .

    Methods

    ·162 patients with chronic schizophrenia from Shanghai Mental Health Center of Huangpu District were randomly divided into CCRT group and control group according to the random number table method. 81 patients in the control group were treated with the conventional antipsychotic drugs, and 81 patients in the CCRT group were given CCRT combined with the conventional antipsychotic treatment. The period of treatments is 12 weeks. The cognitive function by Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), detection of plasma BDNF and TrK-B expression levels by avidinbiotincomplex-Enzyme Linked Immune Sorbent Assay (ABC-ELISA) were assessed at the baseline and at the end of the 12th week. Paired sample t-test was applied to compare the cognitive function, plasma BDNF and TrK-B protein expression levels in the baseline between the two groups, and the repeated measurements variance analysis was used to compare the cognitive function and plasma BDNF and TrK-B protein expression levels between the two groups after treatment and change within the group. The correlation between the improvement of cognitive function and the change of protein expression levels used a general linear model.

    Results

    ·The CCRT group and the control group actually completed 77 cases each. At the baseline, there was no statistically significant difference among cognitive function, BDNF and TrK-B between the two groups; after 12 weeks of treatment, cognitive function (P=0.000), BDNF (P=0.007) and TrK-B (P=0.015) expression level was statistically different between the CCRT and the control group; for CCRT group, cognitive function (P=0.000), BDNF (P=0.002) and TrK-B (P=0.000) expression level was significantly different after 12 weeks of treatment compared with the baseline, but not in the control group. The change of BDNF protein expression level is significantly correlated with the improvement of vocabulary learning (r2=1.598, P=0.019), story retelling (r2=1.495, P=0.038), digital breadth (r2=1.855, P=0.004), story recall (r2=1.459, P=0.047) and attention function (r2=1.673,P=0.012). The change of TrK-B protein expression level is significantly correlated with the improvement of picture naming (r2=1.582, P=0.034).

    Conclusion

    ·The use of CCRT in the adjuvant treatment of antipsychotic treatment for schizophrenia patients has a significant effect on cognitive function. The improvement of some cognitive functions are significantly related to the changes in the plasma BDNF and its TrK-B expression levels.

    Table and Figures | Reference | Related Articles | Metrics
    Spatio-temporal analysis of incidence rate of syphilis in China
    Ting-ting TIAN, Ya-xuan HOU, Yu-qing LI, Hong-jiao QI, Mo CHEN, Mei-xia LÜ
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2021, 41 (5): 648-652.   DOI: 10.3969/j.issn.1674-8115.2021.05.015
    Abstract248)   HTML14)    PDF(pc) (1308KB)(232)       Save
    Objective

    ·To get the spatio-temporal distribution of the syphilis epidemic in 31 provinces, municipalities directly under the Central Government and autonomous regions of the mainland of China in 2017.

    Methods

    ·The data of syphilis incidence in 31 provinces, municipalities and autonomous regions of the mainland of China in 2017 were obtained from the China Public Health Science Data Center, and the time distrbution characteristics of the incidence rates were described. The global Moran′s I index and Anselin local Moran′s I index were used to analyze the spatial cluster characteristics of the syphilis cases, and then space-time scan analysis based on Poisson distribution was used to explore the spatio-temporal distribution characteristics.

    Results

    ·In 2017, the number of syphilis cases in the 31 provinces, municipalities, and autonomous regions of the mainland of China was 475 860, and the incidence rate was 34.49/100 000. Latent syphilis accounted for most of the cases, reaching 76.78% (365 353/475 860). August had the highest incidence rate. For the spatial distribution, Xinjiang Uygur Autonomous Region was the provincial-level unit with the highest incidence rate of syphilis, reaching 91.80/100 000. The incidence rates of latent, secondary, tertiary, and congenital syphilis appeared with positive spatial autocorrelation (all P<0.05). The high-high clusters of secondary and tertiary syphilis appeared in Shanghai, Jiangsu, and Zhejiang (all P<0.05), respectively, while the high-high clusters of congenital syphilis appeared in Xinjiang and Tibet (P=0.000). The results of space-time scan analysis showed that the main cluster appeared from April to September in Fujian, Jiangxi, Zhejiang, Shanghai, Jiangsu, Hunan, Anhui, and Guangdong (P=0.000). Compared with the outside area, the relative risk of syphilis in this cluster was 1.59 times.

    Conclusion

    ·The incidence rate of syphilis in China is relatively high. There are differences in the types and periods of syphilis prevention and control among provinces, municipalities and autonomous regions. From April to September, Fujian, Jiangxi, Zhejiang, Shanghai, Jiangsu, Hunan, Anhui and Guangdong are the key areas for syphilis prevention and control in the mainland of China.

    Table and Figures | Reference | Related Articles | Metrics
    Analysis of prevalence, mortality and disability-adjusted life year rate of diabetes in Chongqing City
    Xian-bin DING, De-qiang MAO, Yan JIAO, Li-ling CHEN, Jie XU
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2021, 41 (1): 78-81.   DOI: 10.3969/j.issn.1674-8115.2021.01.014
    Abstract253)   HTML4)    PDF(pc) (1106KB)(242)       Save
    Objective

    ·To analyze the prevalence, mortality and disability-adjusted life year (DALY) of diabetes in Chongqing City, and provide the evidence for the prevention and treatment of diabetes.

    Methods

    ·The data of diabetes death cases of Chongqing recorded in National Death Registration System in 2018 and the data of diabetes prevalence of Chongqing in 2018 obtained according to Chronic Diseases and Nutrition Monitoring Scheme of Chinese Residents (Trial) were collected and sorted out. SPSS 25.0 software was used to calculate the mortality, standardized mortality, prevalence, standardized prevalence, years of life lost (YLL) rate, years live with disability (YLD) rate and DALY rate of diabetes in Chongqing.

    Results

    ·In 2018, the diabetes mortality and standardized mortality were 16.20/100 000 and 8.82/100 000 in Chongqing, respectively. The mortality of diabetes in women was higher than that in men (χ2=3.99, P=0.045). In 2018, the prevalence and standardized prevalence of diabetes among residents with the age of 15 and above were 17.90% and 9.25%, respectively in Chongqing. The prevalence of diabetes in men was higher than that in women (χ2=18.12, P=0.000). The rates of YLL, YLD and DALY of diabetes in Chongqing were 2.99‰, 7.44‰ and 10.43‰, respectively. The proportions of YLL rate and YLD rate in DALY rate were 28.67% and 71.33%, respectively.

    Conclusion

    ·The disease burden of diabetes in Chongqing is serious, and the mortality and prevalence of diabetes are high. The YLD rate accounts for more than 70% of DALY rate.

    Table and Figures | Reference | Related Articles | Metrics
    Difference in recurrence rates after temporomandibular joint disc repositioning surgery with miniscrew anchor at different developmental stages in adolescents
    Xuehong WANG, Xuzhuo CHEN, Yi MAO, Da SHEN, Shanyong ZHANG
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2022, 42 (2): 173-177.   DOI: 10.3969/j.issn.1674-8115.2022.02.006
    Abstract75)   HTML31)    PDF(pc) (1598KB)(63)       Save
    Objective

    ·Based on magnetic resonance imaging (MRI), adolescents with anterior disc displacement (ADD) of temporomandibular joint treated by temporomandibular joint disc repositioning surgery with miniscrew anchor were studied retrospectively to analyze the difference of postoperative recurrence rate among adolescents at different developmental stages.

    Methods

    ·Adolescent patients (12 years old≤age≤18 years old ) who received disc repositioning from January 2015 to January 2020 in the Department of Oral and Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine were selected and the patients were divided into 2 groups defined by the age of 15. MRIs during at least 6 months follow-up were used to evaluate changes in the disc position and condylar bone. During follow-up, ADD without reduction was considered relapse. The position, length of disc and height of condylar were analyzed quantitatively by modified three-circle method on MRI images and compared by statistical analysis via Software SPSS 25.0. Difference of recurrence rates between the 2 groups was compared by using chi-square test. T test was performed for the difference of condylar growth height between the 2 groups.

    Results

    ·Totally 90 cases were included, including 64 females and 26 males, with the average age of (14.2±1.2) years and follow-up interval of (16.0±12.0) months. Postoperative MRIs showed that all the discs were repositioned. During follow-up, 96.4% (54/56) of the discs in group A (>15 years old) were still in position, whereas 3.6% (2/56) of the discs had relapsed anteriorly. In group B (≤15 years old), 85.2% (75/88) of the discs were still in position, whereas 14.8% (13/88) of the discs had relapsed anteriorly, which was higher than that in group A (P=0.032). The condylar height in group A increased by (1.33±1.29) mm compared with that in group B [(1.79±1.14) mm)]. The condylar height in group B increased more during follow-ups (P=0.036). The degree of anterior disc displacement in group B was greater than that in group A (P=0.002), but there was no significant difference in preoperative disc length between the 2 groups (P=0.585).

    Conclusion

    ·For adolescent patients who received temporomandibular joint disc repositioning surgery with miniscrew anchor, experiencing rapid growth might be a factor affecting the effect of the operation. The postoperative effect of repositioning surgery with miniscrew anchor may be affected by the rapid growth and development of condyle.

    Table and Figures | Reference | Related Articles | Metrics