Archive By Volume

    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) 2012 Vol.32
    Please wait a minute...
    For Selected: Toggle Thumbnails
    Renal protective effect of Cordyceps sinensis on 5/6 nephrectomy-induced renal fibrosis in rats
    GUO Shan-mai, ZHONG Fang, ZHOU Qiao, et al
       2012, 32 (1): 1-.   DOI: 10.3969/j.issn.1674-8115.2012.01.001
    Abstract1366)      PDF(pc) (17623KB)(1202)       Save

    Objective To establish the rat model of 5/6 nephrectomy-induced chronic renal disease, and investigate the protective effect of Cordyceps sinensis on renal fibrosis. Methods Forty-eight male SD rats were randomly divided into sham-operation group (SO group), 5/6 nephrectomy group (OP group) and5/6 nephrectomy + Cordyceps sinensis treatment group (CS group), with 16 rats in each group. Blood and urine samples were collected 4 weeks and 8 weeks after nephrectomy, and rats were sacrificed for renal tissue samples. Serum creatinine (SCr), blood urea nitrogen (BUN), albumin (Alb) and urinary albumin to creatinine ratio (UACR) were measured, pathological changes and ultrastructure changes of renal tissues were observed, the expression of extracellular matrix fibronectin (FN), collagen type Ⅲ (Col-Ⅲ), fibroblast-specific protein 1 (FSP-1) and α-smooth muscle actin (α-SMA) in renal tissues was detected by immunohistochemistry, and the expression of FN, Col-Ⅲ and α-SMA mRNA in renal tissues was determined by RT-PCR. Results Compared with SO group, significant pathological changes were observed in OP group 4 weeks after surgery, which were characterized by infiltration of inflammatory cells, more deposition of extracellular matrix, podocyte foot process effacement, mitochondrial swelling, vacuolization, distorted cristae and autophagosome formation, and these changes were more significant 8 weeks after surgery. Compared with SO group, SCr, BUN and UACR were significantly higher, Alb was significantly lower, the expression of FSP-1, FN, Col-Ⅲ and α-SMA protein and FN, Col-Ⅲ and α-SMA mRNA in renal tissues was significantly higher in OP group 4 weeks after surgery, and these differences were more significant 8 weeks after surgery. Compared with OP group, the pathological changes of renal tissues obviously lessened, mitochondrial damage significantly alleviated, UACR significantly decreased, Alb significantly increased, and the expression of FSP-1, FN, Col-Ⅲ and α-SMA protein and FN, Col-Ⅲ and α-SMA  mRNA in renal tissues significantly decreased in CS group 8 weeks after surgery. Conclusion Cordyceps sinensis ameliorates 5/6 nephrectomy-induced renal fibrosis of rats by relieving mitochondrial damage and reducing deposition of ECM.

    Related Articles | Metrics
       2012, 32 (1): 3-.  
    Abstract969)            Save
    Related Articles | Metrics
    Responsive ability of rats with type 2 diabetes under acute inflammatory state
    HAN Ting-ting, SU Budegerile, HU Yao-min, et al
       2012, 32 (1): 9-.   DOI: 10.3969/j.issn.1674-8115.2012.01.002
    Abstract1532)      PDF(pc) (5716KB)(1068)       Save

    Objective To investigate the differences of responsive ability and blood glucose between rats with type 2 diabetes and normal rats under acute inflammatory state. Methods GK rats with type 2 diabetes and Wistar rats (normal controls) were selected, and critical ill models of sepsis were established by intraperitoneal injection of 5 mg/kg lipopolysaccharide (LPS). Before and after LPS injection, the general state was observed, serum mass concentrations of pro-inflammatory cytokines of tumor necrosis factor α (TNF-α), interleukin 1β(IL-1β) and interleukin 6 (IL-6) and anti-inflammatory cytokine of interleukin 10 (IL-10) were measured, and blood glucose fluctuation was monitored in Wistar rats and GK rats. Results After LPS injection, the general state of Wistar rats was poor, and symptoms such as reduced activity, drowsiness, hair erection and severe diarrhea emerged. However, GK rats stayed in good condition, and did not bear the above symptoms. Before LPS injection, the concentration of blood glucose of GK rats was significantly higher than that of Wistar rats. After LPS injection, the concentrations of blood glucose of Wistar rats and GK rats significantly increased, the range of blood glucose fluctuation of GK rats was smaller than that of Wistar rats, while there was no significant difference in the peak concentration of blood glucose between GK rats and Wistar rats. Before LPS injection, serum mass concentrations of TNF-α and IL-6 of GK rats were significantly higher than those of Wistar rats (P<0.01). The serum mass concentrations of TNF-α, IL-1β, IL-6 and IL-10 after LPS injection were significantly higher than those before LPS injection in GK rats and Wistar rats (P<0.01), and the mass concentrations of TNF-α, IL-1β and IL-10 in Wistar rats were significantly higher than those in GK rats after LPS injection (P<0.01). Conclusion The tolerance to acute inflammation of rats with type 2 diabetes is higher than that of normal rats.

    Related Articles | Metrics
    Effects of erythropoietin on expression of brain-derived neurotrophic factor and tyrosine receptor kinase B after brain injury in mice of different developmental stages
    JIN Bao, ZHANG Yu-cai, CUI Yun
       2012, 32 (1): 14-.   DOI: 10.3969/j.issn.1674-8115.2012.01.003
    Abstract1501)      PDF(pc) (8964KB)(1345)       Save

    Objective To investigate the effects of erythropoietin (EPO) on expression of brain-derived neurotrophic factor (BDNF) and tyrosine receptor kinase B (TrkB)mRNA and protein after brain injury induced by ibotenic acid (Ibo) in mice of different developmental stages. Methods One hundred and twenty KM mice aged 7 d (n=40), 21 d (n=40) and 42 d (n=40) were selected, and those of the same age were randomly divided into Ibo group (n=15), Ibo+EPO group (n=15) and control group (n=10), respectively. In Ibo group, 1 μL Ibo was injected into left hippocampus. In Ibo+EPO group, intraperitoneal injection of 5 000 U/(kg·d)EPO was performed for 5 consecutive days after injection of 1 μL Ibo into left hippocampus. Mice in control group were treated with same amount of normal saline. Y-maze tests were carried out 5 d after model establishment in each group, the pathological changes of neurons in hippocampus were observed with Cresyl Violet staining, the expression of BDNF mRNA and TrkB mRNA in hippocampus was detected by Real-Time PCR, and the expression of BDNF protein and TrkB protein was determined by ELISA. Results After operation, the percentage of mice entering safe arm in Ibo group was significantly lower than that in control group (P<0.05), while the percentage of mice entering safe arm in EPO+Ibo group was significantly higher than that in Ibo group (P<0.05). Light microscopy revealed that degeneration and cell death of neurons in hippocampus occurred in  Ibo group, and the death rates of neurons in hippocampus in Ibo+EPO group and Ibo group were significantly higher than that in control group (P<0.05). However, the pathological changes of Ibo+EPO group were more moderate. The expression of BDNF and TrkB mRNA and protein in Ibo group and Ibo+EPO group was significantly higher than that in control group (P<0.05), and the expression of BDNF and TrkB mRNA and protein in Ibo+EPO group was significantly higher than that in Ibo group (P<0.05). Conclusion The expression of BDNF and TrkB mRNA and protein is significantly up-regulated in hippocampus of mice with Ibo-induced brain injury, which may be a protective reaction. EPO mitigates brain injury induced by Ibo, the mechanism of which may be related to the up-regulation of expression of BDNF and TrkB mRNA and protein.

    Related Articles | Metrics
    Inhibition of aseptic inflammation by gene silencing of tumor necrosis factor-α
    PENG Xiao-chun, CHENG Tao, ZHAO Song, et al
       2012, 32 (1): 21-.   DOI: 10.3969/j.issn.1674-8115.2012.01.004
    Abstract1476)      PDF(pc) (7665KB)(1096)       Save

    Objective To investigate the inhibitory effect of local application of small interfering RNA (siRNA) targeting tumor necrosis factor-α (TNF-α) on wear debris-induced aseptic inflammation. Methods Recombinant lentivirus vector was constructed, and a siRNA targeting TNF-α and a missense siRNA were designed. Air pouches were established and stimulated by polymethylmethacrylate (PMMA) particles for aseptic inflammation. Mice were randomly divided into 3 groups, with 12 mice in each group, and TNF-α siRNA (TNF-α group), missense siRNA (MS group) and PBS (control group) were locally injected into pouches respectively. Mice in each group were sacrificed on the 14th day and 28th day after lentivirus transfection, with 6 rats on each day, and pouch homogenates were obtained. The expression of TNF-α, IL-1β and IL-6 mRNA in pouches was detected by Real-Time PCR, the content of TNF-α protein was determined by ELISA, the thickness of pouch membrane was measured by histological analysis, inflammatory cell counting was performed, and intensity and distribution of green fluorescent protein (GFP) were quantitatively analysed by in vivo bioluminescence imaging. Results The transfection of lentivirus-mediated TNF-α siRNA in vivo significantly decreased the expression of TNF-α, IL-1 and IL-6 mRNA and the content of TNF-α protein in TNF-α group (P<0.01). Histological analysis revealed less inflammatory responses (thinner pouch membrane and decreased cellular infiltration) in TNF-α group (P<0.05 or P<0.01). In vivo bioluminescence imaging indicated the expression of GFP in pouches was locally restrained, and the fluorescence quantity in TNF-α group and MS group was about 5 times higher that in control group. Conclusion Local application of TNF-α siRNA may effectively inhibit wear debris-induced aseptic inflammation, with no systemic adverse effects.

    Related Articles | Metrics
    Synthesis of tropane compounds and their antagonistic activity and tissue selectivity to trachea of rats
    LIU Hui-zhong, OU Mei-xian, YAN Zhong-hong, et al
       2012, 32 (1): 27-.   DOI: 10.3969/j.issn.1674-8115.2012.01.005
    Abstract1461)      PDF(pc) (5147KB)(1267)       Save

    Objective To synthesize a series of tropane derivatives, and investigate their antagonistic activity and tissue selectivity (trachea/heart) to trachea of rats. Methods A series of 3α-acyloxy-6β-acetoxy tropanes were prepared by acetylating 3α-hydroxy-6β-acetoxy tropane (A0). The antagonistic activities of compounds to muscarinic M2 and M3 receptors on isolated heart and trachea of rats were tested by functional assays respectively. Results Eight tropane compounds were prepared. A1-A4 elicited obvious antagonistic activity to heart and trachea, while A5-A8 had no muscarinic antagonistic activity to heart and trachea. A1 had the highest antagonistic parameter (pA2=7.32) to trachea and higher tissue selectivity (trachea/heart)(ΔpA2=1.51).  Conclusion The antagonistic activity and tissue selectivity (trachea/heart) of compounds to trachea of rats may be improved by decreasing the volume of substituting group connected with phenyl in C-3α position of tropane skeleton or increasing the π-electron density of phenyl ring.

    Related Articles | Metrics
    Expression of aquaporin 1 in myocardial tissues of sheep after cardiopulmonary bypass and its relationship with myocardial edema
    YAN Yu-mei, DING Fang-bao, MEI Ju, et al
       2012, 32 (1): 32-.   DOI: 10.3969/j.issn.1674-8115.2012.01.006
    Abstract1396)      PDF(pc) (5913KB)(1204)       Save

    Objective To investigate the expression of aquaporin 1 (AQP1) in myocardial tissues of sheep after cardiopulmonary bypass and its relationship with myocardial edema. Methods Twenty-four healthy adult sheep were randomly divided into normal control group (n=6) and cardiopulmonary bypass group (n=18), and animal samples were obtained at 6 time points (2 h, 6 h, 12 h, 24 h, 48 h and 72 h) after cardiopulmonary bypass in cardiopulmonary group, with 3 sheep at each time point. Real-Time PCR, Western blotting, ELISA and dry/wet method were employed to determine the expression of AQP1 in myocardial tissues of ventricles and severity of myocardial edema at different time points after cardiopulmonary bypass in two groups. Results There existed expression of AQP1 in myocardial tissues of ventricles of sheep in normal control group, and there was no significant difference between myocardial tissues of left ventricles and those of right ventricles (P>0.05). In cardiopulmonary bypass group, there were significant differences in the expression of AQP1 in myocardial tissues among different time points after cardiopulmonary bypass (P<0.05), and the expression tendency of AQP1 mRNA was in line with that of AQP1 protein. The expression of AQP1 mRNA and AQP1 protein in cardiopulmonary bypass group began to decrease after cardiopulmonary bypass, reached the minimum level 6 h after cardiopulmonary bypass, increased from then on, reached the peak level 48 h after cardiopulmonary bypass, and decreased after that time point. There were significant differences in the severity of myocardial edema among different time points after cardiopulmonary bypass (P<0.05). The severity of myocardial edema began to increase after cardiopulmonary bypass, reach the peak level 48 h after cardiopulmonary bypass, and began to decrease after that time point. The relative expression of AQP1 mRNA was positively related to that of AQP1 protein (r=0.971,P<0.05), and AQP1 protein was positively related to the water content in myocardium (r=0.592,P<0.05). Conclusion Cardiopulmonary bypass may influence the expression of AQP1 and severity of myocardial edema in sheep, and there is positive relationship between AQP1 protein and myocardial edema.

    Related Articles | Metrics
    Study on extraction technology of carrot extract containing panaxynol
    YAN Zhong-hong, YANG Ruo-lin, NIU Yin-yao, et al
       2012, 32 (1): 37-.   DOI: 10.3969/j.issn.1674-8115.2012.01.007
    Abstract1326)      PDF(pc) (6301KB)(1425)       Save

    Objective To investigate and optimize the technology of extraction of panaxynol from carrot. Methods Using the concentration of panaxynol as indicator by high performance liquid chromatography (HPLC), the factors that affected the extraction of panaxynol, including extracting parts and method were investigated respectively by single factor method. Based on the preliminary result, the orthogonal test was designed to optimize the extraction technology. Flow cytometry was employed to observe the effect of the optimized extract and panaxynol on leukemia cell line HL60 in vitro. Results The press residue was determined as the raw material of extraction. The optimum conditions were that the ratio of material to liquid (petroleum ether) was 1∶1, times of ultrasonic extraction were three, and the duration of extraction was 2 h. Significant apoptosis of HL60 cells was revealed by flow cytometry after treatment of the extract (40 μg/mL) and panaxynol (5 μmol/L) for 12 h, respectively. Conclusion The optimized extraction method is simple, convenient and reasonably practicable, with which the efficiency of extraction of panaxynol from carrot is increased significantly, and the extract has significant effects on growth inhibition and apoptosis promotion of HL60 cells in vitro.

    Related Articles | Metrics
    Effects of cyclic stretching strain on cytoskeleton of human periodontal ligament cells
    MA Jia-yin, XU Chun, HAO Yi, et al
       2012, 32 (1): 42-.   DOI: 10.3969/j.issn.1674-8115.2012.01.008
    Abstract1147)      PDF(pc) (7447KB)(938)       Save

    Objective To investigate the effects of cyclic stretching strain on the cytoskeleton of human periodontal ligament cells (HPDLCs). Methods HPDLCs were cultured in vitro, and were cyclicly stretched by the strain of 1%, 10% and 20% for 0.5 h, 1 h, 2 h, 4 h, 6 h, 12 h and 24 h, respectively. The morphology of cytoskeleton of HPDLCs was observed by confocal laser scanning microscopy, and figures were collected for analysis with ImagePro Plus 4.5.0.29 software. The area of HPDLCs, ratio of length to width and  integrated fluorescence intensity of cytoskeleton protein F-actin were measured. Results Confocal laser scanning microscopy revealed that with the increase of stretching strain and prolongation of action, HPDLCs gradually arranged in parallel and exhibited paliform, and the direction of arrangement was vertical to that of stretching strain, with prolonged cell body. Figure analysis demonstrated that the area of HPDLCs, ratio of length to width and expression of F-actin changed accordingly after cyclic stretching strain load. Conclusion Cyclic stretching strain may cause changes of cytoskeleton of HPDLCs, which may be related to the level of stretching strain and duration of action.

    Related Articles | Metrics
    Research of glucose metabolism and tissue-targeted insulin sensitivity in two mouse models of hyperlipidemia
    BAI Jie-fei, ZHANG Yao, SU Budegerile, et al
       2012, 32 (1): 48-.   DOI: 10.3969/j.issn.1674-8115.2012.01.009
    Abstract1832)      PDF(pc) (5383KB)(1181)       Save

    Objective To observe the change of glucose metabolism and tissue-targeted (liver and skeletal muscle) insulin sensitivity in mice with hypertriglyceridemia or hypercholesterolemia. Methods Mice fed with normal diet (control group, n=8), lipoprotein lipase gene knockout heterozygous mice (LPL+/-) (hypertriglyceridemia group, n=8) and mice fed with high-fat diet (hypercholesterolemia group, n=8) were selected. Body weight of each group of mice was measured, serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood glucose and fasting insulin were detected, homeostasis model assessment of insulin resistance(HOMA-IR) and insulin sensitivity index (ISI) were calculated, and the change of phosphorylation of serine of Akt473 after insulin stimulation (relative expression of p-Aktser473) in liver and skeletal muscle tissues was determined by Western blotting. Results Serum TG in hypertriglyceridemia group was significantly higher than those in control group and hypercholesterolemia group (P<0.05), and serum TC in hypercholesterolemia group was significantly higher than those in control group and hypertriglyceridemia group (P<0.05). Blood glucose, fasting insulin and HOMA-IR were higher, and ISI was lower in hypertriglyceridemia group than in control group, while there was no significant difference in these parameters between two groups (P>0.05). Blood glucose, fasting insulin and HOMA-IR were significantly higher, and ISI was significantly lower in hypercholesterolemia group than in control group and hypertriglyceridemia group (P<0.05). The relative expression of p-Aktser473 and times of increase in relative expression in liver and skeletal muscle tissues after insulin stimulation in hypertriglyceridemia group and hypercholesterolemia group were significantly lower than those in control group (P<0.05). The increase in relative expression of p-Aktser473 in liver tissues in hypercholesterolemia group was significantly lower than that in hypertriglyceridemia group (P<0.05), and the relative expression of p-Aktser473 in skeletal muscle tissues after insulin stimulation in hypercholesterolemia group was significantly higher than that in hypertriglyceridemia group (P<0.05). Conclusion Mice with hypertriglyceridemia or hypercholesteremia have impaired tissue-targeted insulin sensitivity, and those with hypercholesteremia fed with high-fat diet have more significantly impaired glucose metabolism.

    Related Articles | Metrics
    In vitro construction of tissue-engineered cardiac tissue sheet by small intestinal submucosa with bone mesenchymal stem cells
    HE Yan-zhong, WANG Zhen-yu, ZHENG Jing-hao, et al
       2012, 32 (1): 53-.   DOI: 10.3969/j.issn.1674-8115.2012.01.010
    Abstract1489)      PDF(pc) (9713KB)(1235)       Save

    Objective To explore the feasibility of in vitro construction of tissue-engineered cardiac sheet by small intestinal submucosa (SIS) with bone mesenchymal stem cells (BMSCs). Methods BMSCs separated from rat bone marrow were induced by 5-azacytidine (5-Aza) for 3 weeks, and were seeded on the serosal side of acellular SIS scaffold. The tissue-engineered cardiac tissue sheet was established in vitro after co-culture in dynamic culture system for 2 weeks, and its properties were evaluated by histological, ultrastructural and immunohistochemical examinations. Results After co-culture for 2 weeks, HE staining revealed that the seeded cells were not restricted in the upper area of SIS, forming multiple uniform layers on SIS, and some cells penetrated and migrated into the porous matrix. Scanning electronic microscopy revealed that BMSCs adhered, proliferated and migrated well on SIS, and secreted a large amount of extracellular matrix. Immunohistochemical staining demonstrated that the cells on SIS were cardiomyocyte-like cells with the expression of α-actin, cTnⅠ and connexin-43. Conclusion The engineered cardiac tissue sheet has been successfully constructed by SIS with 5-Aza-induced BMSCs in vitro, and SIS is an ideal bio-scaffold material for cardiac tissue engineering.

    Related Articles | Metrics
    Histological changes of skin and its relationship with wound healing in diabetic nude mice
    HUANG Xin, SHEN Xiang-qian, WU Shou-cheng, et al
       2012, 32 (1): 59-.   DOI: 10.3969/j.issn.1674-8115.2012.01.011
    Abstract1686)      PDF(pc) (6055KB)(1315)       Save

    Objective To observe the histological changes of skin and explore its relationship with wound healing in diabetic nude mice. Methods Diabetic nude mice models were established by intraperitoneal injection of 150 mg/kg STZ. Full-thickness skin of 1.8 cm in diameter was obtained from the dorsum of normal control rats (n=20) and diabetic nude mice (n=20) 4 weeks after model establishment. HE staining was used to observe the histological changes of skin, and the thickness of dermis and epidermis was measured. Picrosirius red staining was employed to analyse the relative content of collagen in dermis. The cutaneous content of glucose was determined by glucose oxidase method. The wound closure rates were calculated 3 d, 5 d, 7 d, 14 d and 21 d after operation. Results The dermis and epidermis of diabetic nude mice were significantly thinner than those of normal controls (P<0.05), and the relative content of collagen in dermis of diabetic nude mice was significantly lower than that of normal controls (P<0.01). The concentration of blood glucose and cutaneous content of glucose of diabetic nude mice were significantly higher than those of normal controls (P<0.01), and there was a positive correlation between cutaneous content of glucose and concentration of blood glucose (r=0.951, P<0.01). The wound closure rates of diabetic nude mice 3 d, 5 d, 7 d, 14 d and 21 d after operation were significantly lower than those in normal controls (P<0.01). Conclusion There are significant histological changes of skin in diabetic nude mice induced by STZ, and these changes may be related to the accumulation of advanced glycation end products, which may lead to the impaired wound healing.

    Related Articles | Metrics
    Isolation, identification and growth characteristics of Porphyromonas gingivalis clinical strains
    LI Yun-peng, LIU Da-li, SHU Rong, et al
       2012, 32 (1): 64-.   DOI: 10.3969/j.issn.1674-8115.2012.01.012
    Abstract4471)      PDF(pc) (6147KB)(2359)       Save

    Objective To isolate and identify Porphyromonas gingivalis (P. gingivalis) from clinical subjects, and analyse the growth characteristics of P. gingivalis. Methods Subgingival plaque samples were collected from patients with chronic periodontitis and periodontally healthy subjects, and anaerobic culture was performed. P. gingivalis strains were identified by PCR and 16S rDNA sequencing, and detection rate was analysed. Bacteria colonies and growth characteristics of the clinical strains of P. gingivalis were observed, and the biological properties were analysed. Results Six P. gingivalis clinical strains named as L2, L3, L4, L5, L11 and L12 were isolated from 146 subgingival plaque samples collected from 35 subjects. The detection rate of P. gingivalis was 27.4% in subgingival plaque samples of periodontally healthy subjects, 86.7% in periodontally healthy sites of patients with chronic periodontitis, 95.6% in periodontal pockets with probing depth of 4-6 mm, and 96.0% in periodontal pockets with probing depth >6 mm. Strains L2, L5, L11 and L12 formed black colonies on BHI plates after culture for 1 week. Strains L3 and L4 formed grey colonies 1 week after culture for 1 week, and formed black colonies after culture for 10 d. Strains L2, L4, L5 and L12 grew to exponential phase 30 h after inoculation, whereas strains L3 and L11 grew to exponential phase 60 h after inoculation. Conclusion P. gingivalis is one of the dominant pathogenic bacteria in periodontal pockets of patients with chronic periodontitis. The detection rate of P. gingivalis in healthy sites of patients with chronic periodontitis is significantly higher than that of periodontally healthy subjects, and the detection rates of P. gingivalis are higher in deeper periodontal pockets. The growth characteristics of P. gingivalis clinical strains vary from each other, indicating that the biological and pathogenic properties of P. gingivalis may be strain-specific.

    Related Articles | Metrics
    Cardiac structure and function in senile patients with essential hypertension
    HU Jun, ZHU Fu, XIE Jun, et al
       2012, 32 (1): 69-.   DOI: 10.3969/j.issn.1674-8115.2012.01.013
    Abstract1499)      PDF(pc) (4078KB)(1102)       Save

    Objective To investigate the effects of age and hypertension on cardiac structure and function in senile patients with essential hypertension. Methods One hundred and fifty hospitalized patients with essential hypertension were divided into senile hypertension group (n=84), senile non-hypertension group (n=18) and elderly hypertension group (n=48) according to age (senile, ≥80 years old; elderly, 60 to 80 years old) and conditions of hypertension. The baseline conditions were recorded, the blood biochemical parameters were measured, and 24 h ambulatory blood pressure monitoring was performed. Cardiac ultrasonography was conducted, and parameters of cardiac structure and function were measured and calculated. The correlation of disease course of hypertension with cardiac structure and function was analysed. Results There were significant differences in mean age, body mass index, total cholesterol, high-density lipoprotein and low-density lipoprotein between senile hypertension group and elderly hypertension group (P<0.05 or P<0.01). The 24 h systolic blood pressure and diastolic blood pressure in senile hypertension group were significantly higher than those in senile non-hypertension group (P<0.01), and there were significant differences in left ventricular mass, left ventricular mass index and left ventricular posterior wall thickness between these two groups (P<0.01), while there was no significant difference in parameters of cardiac structure between senile hypertension group and elderly hypertension group (P>0.05). The parameters of cardiac function in senile hypertension group were significantly different from those in elderly hypertension group and senile non-hypertension group (P<0.05). Correlation analysis revealed that the disease course of hypertension was positively related to the parameters of cardiac structure (P<0.05 or P<0.01), while was negatively related to the parameters of cardiac function (P<0.05). Conclusion In senile patients, hypertension may cause more serious left ventricular hypertrophy, increased cardiac mass and decreased cardiac systolic function. With the prolongation of disease course of hypertension, more severe left ventricular hypertrophy and left atrial and ventricular enlargement occur, and even serious left ventricular systolic function takes place. Decreasing blood pressure stably in a rational range is advisable for senile patients with essential hypertension.

    Related Articles | Metrics
    Photoelastic analysis of stress distribution of complete dentures with lingualized occlusion (routine complete denture load)
    JIN Wen-zhong, JIAO Ting, PENG Yan, et al
       2012, 32 (1): 73-.   DOI: 10.3969/j.issn.1674-8115.2012.01.014
    Abstract1687)      PDF(pc) (4251KB)(1238)       Save

    Objective To investigate the stress distribution of supporting tissues in complete dentures with lingualized occlusion and anatomic occlusion under centric and lateral stress load. Methods Three-dimensional photoelastic experiment mothod was adopted, and photoelastic models of epoxy were fabricated according to the Cawood V edentulous model. Complete dentures with lingualized occlusion and anatomic occlusion were prepared respectively. After frozen under centric and lateral stress load, the stress distribution of supporting tissues was analysed and compared. Results Under centric and lateral stress load, lingualized occlusion conveyed more stress to lingual side than anatomic occlusion in routine complete dentures, and the occlusion stress in lingualized occlusion was better distributed. Conclusion For patients with severe absorbed alveolar ridge undergoing complete denture restoration, lingualized occlusion should be considered in first place to increase the stability of dentures.

    Related Articles | Metrics
    Metabolic profiling research of primary liver tumor based on multiple discriminant analysis
    LI Fen, ZHAO Ai-hua, YANG Jing-lei, et al
       2012, 32 (1): 77-.   DOI: 10.3969/j.issn.1674-8115.2012.01.015
    Abstract1550)      PDF(pc) (5618KB)(1225)       Save

    Objective To compare the diagnosis performance of discriminant analysis methods through application on clinical serum samples. Methods Linear discriminant analysis (LDA), quadratic discriminant analysis (QDA) and logistic discriminant analysis (LogDA) were applied to metabolic profiling analysis deriving from clinical serum samples of 109 healthy controls, 87 patients with benign liver tumor and 31 patients with malignant liver tumor. The diagnosis performance of these three methods was compared in discrimination of healthy controls and patients with liver tumor(benign tumor and malignant tumor) and in discrimination of patients with benign tumor and those with malignant tumor. Results Based on current clinical metabolic profiling data, the effectiveness of all these three methods worked better in discrimination of healthy controls and patients with liver tumor than in discrimination of patients with benign tumor and those with malignant tumor. The overall performance of QDA was superior to LDA and LogDA, with the precision of 87.67% in discrimination of healthy controls and patients with liver tumor and the precision of 67.80% in discrimination of patients with benign tumor and those with malignant tumor. Conclusion QDA outperforms LDA and LogDA in processing primary liver tumor metabolic data.

    Related Articles | Metrics
    Clinical features and efficacy of 131I remnant ablation of differentiated thyroid carcinoma complicated with hyperthyroidism
    SHENG Shi-wei, LU Han-kui, CHEN Li-bo, et al
       2012, 32 (1): 82-.   DOI: 10.3969/j.issn.1674-8115.2012.01.016
    Abstract1610)      PDF(pc) (4126KB)(1171)       Save

    Objective To investigate the clinical features and efficacy of 131I remnant ablation of differentiated thyroid carcinoma complicated with hyperthyroidism. Methods The clinical data of 1 233 patients with DTC hospitalized between January 2000 and December 2009 were retrospectively analysed, among whom 13 had history of hyperthyroidism. Thyroxine tablets administration withdrew before admission, and iodine-free diet was carried out for 3 to 6 weeks. Before 131I treatment, serum thyroglobulin, thyroglobulin antibodies, thyroid hormones (TT3, TT4, FT3, FT4), thyroid stimulating hormone (TSH), blood routine, liver and renal function were examined, and the disease condition was evaluated by neck ultrasound. Four to 6 months after 131I treatment, all patients were followed up for the treatment efficacy. Results The positive rate of thyroid ultrasonography for DTC complicated with hyperthyroidism was 61.54% (8/13), and those of thyroid scintigraphy and fine needle aspiration biopsy were 33.33% (2/6)and 75.00% (3/4) respectively. The total dose of 131I used for DTC complicated with hyperthyroidism was significantly lower than that for DTC (P<0.05). Four to six months after 131I remnant ablation, 11of the 13 patients (84.26%) had successful ablation of residual thyroid tissues after first administration of 131I, which was significantly higher than that of patients with single DTC (55.90%). Conclusion Thyroid ultrasonography, thyroid scintigraphy and fine needle aspiration biopsy remain important means in examining patients with DTC complicated with hyperthyroidism. 131I treatment for ablation of residual thyroid tissues in patients with DTC complicated with hyperthyroidism may yield better efficacy than in patients with single DTC.

    Related Articles | Metrics
    Clinical study of atrial fibrillation treated by radiofrequency ablation modified maze procedure
    ZENG Ying-ou, ZHANG Wei, YE Wei, et al
       2012, 32 (1): 85-.   DOI: 10.3969/j.issn.1674-8115.2012.01.017
    Abstract1118)      PDF(pc) (5219KB)(1342)       Save

    Objective To investigate the outcomes of radiofrequency ablation modified maze procedure during open-heart surgery for patients with atrial fibrillation, and analyse the factors influencing the efficacy. Methods One hundred and sixty-one patients with atrial fibrillation undergoing cardiovascular surgery were treated on the basis of modified Cox's Maze Ⅲ. Twenty-seven patients with mild tricuspid valve disease were treated with simple left atrial ablation (single room ablation group), and 134 patients with moderate to severe tricuspid valve disease underwent double room ablation (double room ablation group). The success rate of ablation, postoperative complications, and impact factors for postoperative recovery of sinus rhythm were analysed in two groups. Results All patients were followed up for an average of (24.64±12.92) months after surgery. Electrocardiographic monitoring indicated that sinus rhythm was recovered in 69.57% of patients immediately after surgery, and 14.91% of patients had nodal rhythm. The percentages of patients with sinus rhythm were 75.78% and 81.37% at discharge and 6 months after operation respectively. The recovery rate of sinus rhythm in single room ablation group was 77.78%, and that in double room ablation group was 82.09%, and there was no significant difference between two groups (P>0.05). Multivariate analysis revealed that cardiac function classification, ejection fraction after surgery, time of cardiopulmonary bypass and time of aortic cross-clamping were main factors affecting recovery of sinus rhythm (P<0.05). Conclusion Atrial fibrillation with mild tricuspid valve disease should be treated with simple left atrial ablation, atrial fibrillation with moderate to severe tricuspid valve disease should be managed with double atrial ablation, and both may yield similar outcomes. Cardiac function classification before surgery, ejection fraction after surgery, time of cardiopulmonary bypass and time of aortic cross-clamping are the main predictors for recovery of sinus rhythm after ablation.

    Related Articles | Metrics
    Influence of age on neuromuscular block effect of cisatracurium
    ZHANG Xiao-yi, WANG Shan-juan, ZHANG Ma-zhong, et al
       2012, 32 (1): 90-.   DOI: 10.3969/j.issn.1674-8115.2012.01.018
    Abstract1164)      PDF(pc) (3365KB)(1200)       Save

    Objective To investigate the neuromuscular block effect of intravenous injection of two times of 95% effective dose (ED95) of cisatracurium in patients with different ages. Methods Sixty patients with ASA Ⅰ-Ⅱ were divided into children group (3 to 7 years old), youth group (18 to 65 years old) and elder group (≥ 65 years old), with 20 patients in each group. Cisatracurium of two times of ED95 was injected during anesthesia induction, neuromuscular block was monitored with TOF Watch acceleration instrument, and the time of neuromuscular block onset, duration of blockade maintenance, duration of clinical action, duration of in vivo action and neuromuscular block recovery index were obtained. Results There was no significant difference in trachea intubation grading among three groups (P>0.05). The time of neuromuscular block onset, duration of blockade maintenance, duration of clinical action and duration of in vivo action in children group were significantly shorter than those in youth group and elder group (P<0.05). There was no significant difference in neuromuscular block recovery index among three groups (P>0.05). Conclusion Children are more sensitive than adults to cisatracurium, with faster neuromuscular block onset, shorter duration of blockade maintenance and shorter duration of clinical action. The neuromuscular block recovery time is not related to age, and two times of ED95 of cisatracurium is safe and effective for patients with different ages.

    Related Articles | Metrics
    Contrast analysis of duodenoscopic treatment and traditional treatment for bile leakage after cholecystectomy
    LOU Xiao-lou, YANG Jian-jun, LIU Wen-yong, et al
       2012, 32 (1): 93-.   DOI: 10.3969/j.issn.1674-8115.2012.01.019
    Abstract1156)      PDF(pc) (3209KB)(1117)       Save

    Objective To compare the effects of duodenoscopic treatment and traditional treatment on bile leakage after cholecystectomy. Methods Thirty-five patients with bile leakage after cholecystectomy were divided into duodenoscopic treatment group (managed with endoscopic retrograde cholangiopancreatography method, n=17) and traditional treatment group (managed with conservative therapy and surgery, n=18). The percentages of bile leakage location confirmation, time of bile leakage closure, re-operation rates and prevalences of complications were compared between two groups. Results The percentage of bile leakage location confirmation in duodenoscopic treatment group was significantly higher than that in traditional treatment group (76.47% vs 38.89%, P<0.05), the time of bile leakage closure in duodenoscopic treatment group was significantly shorter than that in traditional treatment group [(13±3) d vs (23±5) d, P<0.05], the re-operation rate in duodenoscopic treatment group was significantly lower than that in traditional treatment group (11.76% vs 38.89%, P<0.05), and the prevalence of complications in duodenoscopic treatment group was significantly lower than that in traditional treatment group (17.65% vs 38.89%, P<0.05). Conclusion Compared with traditional treatment, duodenoscopic treatment for bile leakage after cholecystectomy is less invasive, more safe and more effective.

    Related Articles | Metrics
    Role of total cholesterol to high-density lipoprotein-cholesterol ratio in prediction of hypertension in mid-aged and young people in Chongqing
    QIU Lei, ZHONG Xiao-ni, SONG Wen-feng, et al
       2012, 32 (1): 96-.   DOI: 10.3969/j.issn.1674-8115.2012.01.020
    Abstract2660)      PDF(pc) (4883KB)(1395)       Save

    Objective To investigate the role of different serum lipid parameters in prediction of hypertension in mid-aged and young people in Chongqing. Methods The data of 20 000 subjects undergoing health examinations in Health Examination Center of the First Affiliated Hospital of Chongqing Medical University in 2009 were collected. The correlation of triglyceride (TG), serum total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C) and non-high-density lipoprotein-cholesterol (Non-HDL-C) with systolic blood pressure (SBP) and diastolic blood pressure (DBP) was explored, and the relationship among different serum lipid parameters were analysed. Serum lipid parameters with strong correlation with blood pressure were selected, and role of these parameters and their different combinations in prediction of area under receiver operator characteristic (ROC) curve of subjects with hypertension was analysed. Results Health examinations revealed that three were 3 102 patients with hypertension, and the prevalence was 15.51%. Among these patients, there were 2 294 males and 808 females, and the prevalence of males was significantly higher than that of females (20.43% vs 9.21%,P<0.05). After adjustment for age and sex of patients with hypertension, TC/HDL-C had the strongest correlation with blood pressure, followed by LDL-C/HDL-C and Non-HDL-C. TC/HDL-C had strong correlation with LDL-C/HDL-C, Non-HDL-C, HDL-C, TG, LDL-C and TC (r=0.926, 0.784, -0.753, 0.724, 0.715 and 0.642 respectively; P<0.001 for all). All the areas under ROC curve of hypertension predicted by TC/HDL-C, LDL-C/HDL-C, Non-HDL-C, TG and their different combinations were >0.5, and the area under ROC curve predicted by TC/HDL-C was the largest. For the same serum lipid parameter and its combinations, areas under ROC curve of females were significantly larger than those of males (P<0.05). Conclusion C/HDL-C may be the optimal serum lipid parameter in prediction of hypertension in Chongqing mid-aged and young people, especially for females.

    Related Articles | Metrics
    Bibliometric analysis of Journal of Shanghai Jiaotong University (Medical Science) during 2006 to 2010
    QIU Xiao-chun, ZHOU Zhu-feng
       2012, 32 (1): 101-.   DOI: 10.3969/j.issn.1674-8115.2012.01.021
    Abstract1507)      PDF(pc) (6385KB)(1510)       Save

    Objective To carry out the bibliometric analysis of Journal of Shanghai Jiaotong University (Medical Science), and evaluate its academic status among domestic academic journals. Methods Based on China National Knowledge Infrastructure (CNKI), bibliometric analysis was conducted on the papers published on Journal of Shanghai Jiaotong University (Medical Science) during 2006 to 2010. The authors, affiliations, foundations, cited papers and citation frequencies were statistically analysed, and h-index was calculated for the journal. Results A total of 2 152 papers were published on the journal during 2006 to 2010. Twenty-four authors published no less than 15 papers, all of whom had senior title or were academic leaders. Twelve first authors published no less than 6 papers. Papers with foundations support accounted for 19.2% of the total. One thousand and thirteen papers were cited for no less than 1 time, 13 papers were cited for no less than 10 times, and the average citation frequency was 0.98. The discipline with the highest citation frequency was oncology. Ruijin Hospital, Renji Hospital and Xinhua Hospital headed the number of papers and citation frequency. The h-index of the journal was 11. Conclusion There is a relative stable core author group for Journal of Shanghai Jiaotong University (Medical Science), and the citation frequency and h-index of the journal are at higher levels among domestic academic journals.

    Related Articles | Metrics
    Research progress of olive oil lipid emulsion
    WANG Ying, CAI Wei
       2012, 32 (1): 107-.   DOI: 10.3969/j.issn.1674-8115.2012.01.022
    Abstract1753)      PDF(pc) (5172KB)(1464)       Save

    Lipid emulsion has been applying in parenteral nutrition ever since 1960s, and has been using in combination with glucose for energy supplement. The lipid emulsion most commonly used now is soybean oil, while there is an opinion that soybean oil lipid emulsion is not optimal to patients. Now a new lipid emulsion prepared from a mixture of 80% olive oil and 20% soybean oil containing a low proportion (20%) of polyunsaturated fatty acids and 60% of monounsaturated fatty acids is available, which may not lead to essential fatty acid deficiency and can alleviate the risk of lipid peroxidation after long-term application. This paper reviews the research progress of olive oil lipid emulsion in vitro studies, animal experiments and clinical investigations.

    Related Articles | Metrics
    Homing of mesenchymal stem cells after infusion in vivo
    SHI Xiao-feng, ZHU Yan
       2012, 32 (1): 111-.   DOI: 10.3969/j.issn.1674-8115.2012.01.023
    Abstract1291)      PDF(pc) (5184KB)(1360)       Save

    Mesenchymal stem cells (MSCs) can be isolated from several tissues,  and can also differentiate into disparate tissues. MSCs bear some capabilities such as immunosuppression, tissue repair, accelerating hematopoietic recovery and reducing graft versus host disease(GVHD), which make MSCs the focus of researches. However, the trafficking, homing and survival of MSCs after infusion into animals and their influential factors are not clear. After reviewing  papers, here we reveal some factors influencing homing of MSCs in vivo such as filtration of lung, quantity and culture condition of MSCs and pathologic status of tissues, among which pathologic status (severity of tissue injury) plays a critical role. MSCs preferentially home to sites of tissue injury, the trafficking direction is in line with the chemokine density gradient, and this progress is associated with many ligands and their receptors. Comprehension of homing of mesenchymal stem cells after infusion in vivo may contribute to providing theoretical foundation for clinical utilization of MSCs.

    Related Articles | Metrics
    Research progress of pathogenesis of diabetic peripheral neuropathy
    SHI Jun, ZHANG Wen-chuan
       2012, 32 (1): 116-.   DOI: 10.3969/j.issn.1674-8115.2012.01.024
    Abstract1969)      PDF(pc) (4197KB)(2257)       Save

    Diabetic peripheral neuropathy (DPN) is a common complication of diabetes, whose pathogenesis is mostly related to metabolism pathway and growth factors. Metabolism pathway includes activation of polyol pathway, hexosamine pathway and protein kinase C pathway and production of advanced glycation end products (AGEs). Oxidative stress may indirectly harm the neurons and axons through the pathways mentioned above, and can directly injure the nerve cells as well. Growth factors are mainly associated with the synthesis and apoptosis of Schwann cells. Surgical treatment may yield favorable outcomes for DPN, and early diagnosis and treatment may effectively hinder the deterioration and development of DPN and reduce the rates of infection and amputation for affected limbs. This paper introduces the pathogenesis of DPN on the basis of metabolism pathway and growth factors.

    Related Articles | Metrics
    Clinical and imaging features of patients with severe influenza A (H1N1)
    CAI Xiao-ting, SHI Guo-chao
       2012, 32 (1): 120-.   DOI: 10.3969/j.issn.1674-8115.2012.01.025
    Abstract1310)      PDF(pc) (5257KB)(1368)       Save

    Objective To investigate the imaging and clinical features of patients with severe influenza A (H1N1). Methods Thirteen patients suffering from severe influenza A (H1N1) were admitted to Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine from December 2009 to February 2010, among whom 10 were males and the other 3 were females, with the median age of 50 years (24 to 59 years). The clinical features, laboratory findings, imaging features, treatment and outcomes of these patients were retrospectively analysed. Results The most common symptoms were fever and cough. The laboratory findings were decrease of the numbers of white blood cells, especially lymphocytes, and increase of lactate dehydrogenase (LDH) level. The major findings of chest radiograph and CT were bilateral ground-glass opacities and areas of consolidation. After anti-virus, corticosteroids, antibiotics and supporting therapy, the symptoms of 11 patients relieved, 1 patient had fungal infection and 1 patient died. Conclusion The major imaging findings of patients with severe influenza A are bilateral mixed interstitial or alveolar infiltrating shadows. The disease progresses quickly and may lead to death, while early diagnosis and early treatment may achieve better therapeutic effects.

    Related Articles | Metrics
    Comparison of effects of propofol and thiopental sodium on intrinsic optical imaging intensity
    REN Rong-rong, ZHAO Xuan
       2012, 32 (1): 124-.   DOI: 10.3969/j.issn.1674-8115.2012.01.026
    Abstract1178)      PDF(pc) (3619KB)(1042)       Save

    Objective To investigate the effects of propofol and thiopental sodium on intrinsic optical imaging intensity of macaques. Methods Six macaques were divided into propofol group and  thiopental sodium group, with 3 macaques in each group. Intramuscular injection of 15 mg/(kg·h) ketamine was performed for anesthesia induction in two groups, and 1.5 mg/(kg·h) thiopental sodium, 0.15 mg/(kg·h) vecuronium, 1%-2% isoflurane, 0.5-2 L/min nitroxide and 1-2 L/min oxygen were used to maintain anesthesia in thiopental sodium group, while 5 mg/(kg·h) propofol, 0.15 mg/(kg·h) vecuronium, 1%-2% isoflurane, 0.5-2 L/min nitroxide and 1-2 L/min oxygen were used to maintain anesthesia in propofol group, and  the vital signs of macaques were recorded. Macaques in two groups were treated with visual stimulus of sinusoidal grating, with the direction of 0° and 90°, visual cortex imaging signals of macaques were detected, and mean imaging intensity was calculated. Results There was no significant difference in heart rates, oxygen saturation, end-tidal carbon dioxide partial pressure, temperature and mean imaging intensity between two groups (P>0.05). Conclusion There is no significant difference in intrinsic optical imaging intensity between thiopental sodium and propofol.

    Related Articles | Metrics
    Effect of pre-operative oral preparation on incidence of ventilator-associated pneumonia after operation
    ZHENG Wei-yan, SUN Yi, HE Zhen-zhou
       2012, 32 (1): 127-.   DOI: 10.3969/j.issn.1674-8115.2012.01.027
    Abstract1459)      PDF(pc) (4184KB)(1328)       Save

    Objective To investigate the effect of pre-operative oral preparation on incidence of ventilator-associated pneumonia (VAP) after cardiopulmonary bypass surgery in elderly patients. Methods Ninety-five patients (≥65 years old) undergoing cardiopulmonary bypass surgery were randomly divided into experiment group (n=48) and control group (n=47). For experiment group, besides conventional preoperative preparation, gargle mouthwash was used after three meals and before sleep on the day before surgery day and before surgery on the surgery day. Besides conventional preoperative preparation, no oral preparation was performed in control group. Tracheal intubation was conducted during anesthesia, oropharyngeal swab samples and sputum samples were collected before intubation and after intubation, and bacteria culture and identification were carried out. The incidences of  VAP after operation were compared between two groups. Results The oropharyngeal infection rate in experiment group was significantly lower than that in control group in oropharyngeal swab culture and sputum culture (P<0.05). The incidences of early-onset VAP and total VAP in experiment group were significantly lower than those in control group (P<0.05), while there was no significant difference in the incidence of late-onset VAP between two groups (P>0.05). Conclusion Pre-operative oral preparation can decrease the incidence of early-onset VAP in elderly patients undergoing cardiopulmonary bypass surgery.

    Related Articles | Metrics
    Metabotropic glutamate receptor 1/5 agonist improves doxorubicin-induced renal injury in mice
    TAO Hua, GU Le-yi, WANG Li-hua, et al
       2012, 32 (10): 1283-.   DOI: 10.3969/j.issn.1674-8115.2012.10.001
    Abstract977)      PDF(pc) (747KB)(1315)       Save

    Objective To investigate the effects of selective agonist for metabotropic glutamate receptors 1 and 5 (mGluR1/5), (S)-3,5-dihydroxyphenylglycine (DHPG), on the doxorubicin (ADR)-induced nephrosis in mice. Methods Eighteen 6-week-old male BALB/c mice were randomly divided into control group, ADR group (nephrosis model established by intravenous injection of ADR) and ADR+DHPG group (pretreatment with DHPG injection before model establishment), with 6 mice in each group. Twenty-four-hour urinary albumine levels were measured by enzyme-linked immunosorbent assay (ELISA), and the foot processes effacement and the width of foot processes were determined with electron microscopy. Laser confocal microscope with immunofluorescence staining was employed to detect the expression of nephrin and desmin, and the expression of WT-1 in glomerulus was determined by immunohistochemistry. Besides, the apoptosis of podocytes was detected by TUNEL staining. Results Twenty-four-hour urinary albumine levels were (1.67±0.22) mg, (22.55±2.34) mg and (8.23±2.74) mg in control group, ADR group, and ADR+DHPG group respectively, and there were significant differences among groups (P<0.01). Compared with ADR group, the width of foot processes in ADR+DHPG was shorter, the expression of desmin was lower, and the expression of nephrin was higher. The number of WT-1 positive cells was negatively related to the number of apoptotic cells in glomerulus (R2=0.8 482,P<0.05), and the number of apoptotic podocytes in ADR+DHPG group was significantly smaller than that in ADR group (P<0.01). Conclusion Selective mGluR 1/5 agonist DHPG may inhibit ADRinduced foot processes fusion of podocytes and decreased expression of nephrin, reduce cell apoptosis, and attenuate urinary albumine in ADR-induced nephrosis in mice.

    Related Articles | Metrics
    Effects of ultrasound exposure and sodium butyrate on transfection of adeno-associated virus to rat retinal pigment epithelial cells
    LI Hong-li, DU Lian-fang, LI Feng-hua, et al
       2012, 32 (10): 1288-.   DOI: 10.3969/j.issn.1674-8115.2012.10.002
    Abstract1390)      PDF(pc) (492KB)(1024)       Save

    Objective To investigate the role of ultrasound exposure and sodium butyrate in transfection of type 2 recombinant adeno-associated virus carrying enhanced green fluorescent protein (rAAV2-EGFP) to rat retinal pigment epithelial (RPE) cells. Methods Rat RPE cells were incubated in 24-well plates, and were transfected with rAAV2-EGFP (control group), rAAV2-EGFP+ultrasound exposure (ultrasound exposure group, ultrasound exposure for 60 s with ultrasound intensity of 0.5 and 1 W/cm2) and rAAV2-EGFP+different concentrations of sodium butyrate (sodium butyrate group) respectively. Forty-eight hours after transfection, the expression of EGFP in the transfected cells was observed by inverted microscopy, and the transfection efficiency (percentage of EGFP positive cells) was detected by flow cytometry. Twenty-four hours after transfection, the cell proliferation was determined by MTS method. Results Flow cytometry indicated that the percentages of EGFP positive cells in ultrasound exposure group and sodium butyrate group were significantly higher than that in control group (P<0.05), and the increase in transfection efficiency in sodium butyrate group was significantly higher than that in ultrasound exposure group (P<0.05). Cell proliferation detection revealed that the absorbance of the transfected cells in ultrasound exposure group was significantly higher than that in sodium butyrate group (P<0.05). Conclusion Both sodium butyrate and ultrasound exposure can increase the transfection efficiency of rAAV2-EGFP to rat RPE cells, and the effect of sodium butyrate is more significant, while ultrasound exposure has less inhibition effect on cell proliferation.

    Related Articles | Metrics
    Effect of 5-hydroxytryptamine on activity of bladder afferent nerves in rats
    SUN Qiao, SUN Bi-ying, LUO Ping, et al
       2012, 32 (10): 1292-.   DOI: 10.3969/j.issn.1674-8115.2012.10.003
    Abstract970)      PDF(pc) (1043KB)(1155)       Save

    Objective To investigate the effect of 5-hydroxytryptamine (5-HT) on the spontaneous and mechanosensitive activity of bladder afferent nerves. Methods The effects of intravesical instillation of different concentrations of 5-HT on the spontaneous and distension-induced pelvic afferent nerve activity were observed in the isolated rat bladder/pelvic nerve preparations. Results At rest, with the bladder empty, there was little background activity (<10 imp/s) in the pelvic nerve branch innervating the bladder. During ramp distension, the afferent nerve activity increased progressively as the intravesical pressure rose. Intraluminal instillation of 0.1 mL 5-HT (1 to 30 μmol/L) caused an increase of the ongoing afferent discharge and potentiated the low threshold (0-15 mmHg) mechanosensory responses in a concentration-dependent manner. Conclusion 5-HT directly activates low threshold bladder afferent fibers in the pelvic nerve, and can potentiate their mechanosensitivity.

    Related Articles | Metrics
    Neuroprotective effects of Catalpol on BV2 cells injured by MPP +
    WANG Zi-mei, XU Gang, ZHANG Yong-fang, et al
       2012, 32 (10): 1296-.   DOI: 10.3969/j.issn.1674-8115.2012.10.004
    Abstract1125)      PDF(pc) (457KB)(1075)       Save

    Objective To explore the role of cerebral dopamine neurotrophic factor (CDNF) in the protection effect of Catalpol on the injured dopaminergic glial cells. Methods ①Mouse glial BV2 cells were randomly divided into control group, Catalpol group, 1-methyl-4-phenylpyridinium (MPP+) model group and MPP+ model+Catalpol group. The expression of CDNF mRNA in BV2 cells 0, 6, 24 and 48 h after treatment was detected by RT-PCR. ② BV2 cells were treated with 0.1, 1 and 10 μmol/L Catalpol respectively, MPP+ was added 24 h later, and the expression of CDNF protein was determined by Western blotting 48 h later. Normal cells were served as control group, and those without pretreatment with Catalpol were established as MPP+ model group. ③Control group, MPP+ model group, Catalpol group, anti-CDNF antibody group and Catalpol+ anti-CDNF antibody group were established, all groups were treated with MPP+ except control group, and the effect of Catalpol on dopamine uptake after CDNF antibody blockade was examined by [3H]-dopamine radiometry. Results ①Compared with control group, the expression of CDNF mRNA in Catalpol group and MPP+ model group was not significantly changed 0 to 72 h after administration of MPP+. However, the expression of CDNF mRNA in MPP+ model+ Catalpol group was significantly higher than that in MPP+ model group 48 h after administration of MPP+ (P<0.01). ②The expression of CDNF protein in MPP+ model group was significantly lower than that in control group 48 h after administration of MPP+ (0.679±0.013 vs 1.009±0.015, P<0.001). The expression of CDNF protein in BV2 cells after pretreatment with 10 μmol/L Catalpol (0.812±0.011) was significantly higher than that in MPP+ model group (P<0.01), while the expression of CDNF protein in BV2 cells after pretreatment with 0.1 and 1 μmol/L Catalpol was not significantly changed compared with MPP+ model group. ③The dopamine uptake in MPP+ model group was significantly lower than that in control group (63.5±2.5 vs 99.9±0.8, P<0.01). The dopamine uptake in Catalpol group (87.2±2.4) was significantly higher than that in MPP+ model group (P<0.01). The dopamine uptake in Catalpol+anti-CDNF antibody group (73.6±2.7) was significantly lower than that in Catalpol group (P<0.01), while was higher than that in anti-CDNF antibody group (P<0.05). Conclusion The neuroprotection effect of Catalpol against MPP+ toxicity may be associated with the upregulation of expression of CDNF mRNA and protein in BV2 cells.

    Related Articles | Metrics
    Molecular mechanisms of substance P-induced monocyte chemoattractant protein-1 secretion in fibroblasts under high glucose culture condition
    JIA Zhi-gang, FANG Yong, YAO Min, et al
       2012, 32 (10): 1302-.   DOI: 10.3969/j.issn.1674-8115.2012.10.005
    Abstract1171)      PDF(pc) (569KB)(958)       Save

    Objective To investigate the molecular mechanisms of substance P (SP)-induced monocyte chemoattractant protein-1 (MCP-1) secretion in fibroblasts under high glucose culture condition. Methods Human skin fibroblasts were cultured in high glucose DMEM medium, and were incubated with 10 nmol/L SP for different time. The expression of inhibitor α of nuclear factor kappa B (NF-κB)(IκBα), phosphonated IκBα (p-IκBα) and phosphonated NF-κB subunit p65 (p-NF-κBp65) in cells was detected by Western blotting, and the expression and translocation of p-NF-κBp65 was observed by immunofluorescence method. Human skin fibroblasts cultured in high glucose DMEM medium were divided into control group, SP group and SP+NF-κB inhibitor (MG132) group (pretreatment with MG132 for 60 min), the expression of p-NF-κBp65 in cells was detected in each group, and the concentration of MCP-1 in the supernatant was determined by ELISA. Results The expression of IκBα significantly decreased, and that of p-IκBα significantly increased in fibroblasts with the time increase of treatment with SP (P<0.05). The expression of p-NF-κBp65 significantly increased after treatment with SP (P<0.05), which was in a time-dependent manner. The expression of p-NF-κBp65 in nuclei significantly increased after treatment with SP observed under laser confocal microscope with immunofluorescence staining. The expression of p-NF-κBp65 in cells and the concentration of MCP-1 in the supernatant in SP+MG132 group were significantly lower than those in control group (P<0.05). Conclusion The mechanisms of SP-induced MCP-1 secretion in fibroblasts may be related to the activation of NF-κB signalling pathway.

    Related Articles | Metrics
    Design, preparation and synchronous transplantation experiments of laser micropore porcine acellular dermal matrix
    ZENG Tao-fang, LUO Xu, XIN Guo-hua, et al
       2012, 32 (10): 1307-.   DOI: 10.3969/j.issn.1674-8115.2012.10.006
    Abstract1021)      PDF(pc) (940KB)(1144)       Save

    Objective To prepare laser micropore porcine acellular dermal matrix (LPADM), a new type of dermal substitute, and to verify the healing effect on full-thickness skin defect. Methods Splitthickness dermis of the healthy white pigs was harvested and treated by high-sodium-SDS method, and the porcine acellular dermal matrix (ADM) was obtained. Besides, LPADM was prepared using laser micropore technology on the dermis, followed by the procedure of high-sodium-SDS acellular treatment. The physical properties of LPADM were evaluated. Four full-thickness skin wounds (2.0 cm×2.0cm) were created on each dorsum of 30 SD rats. Group L (LPADM grafted with the split-thickness autograft), group A (nonporous ADM grafted with the split-thickness autograft), group F (full-thickness autograft) and group C (split-thickness autograft) were divided on the basis of different grafts used through one-step method. Six rats were randomly selected on day 3, 5, 7, 10 and 14 after surgery, the survival of skin graft was observed, and histological examination was conducted after sacrifice. Results The well-prepared micropore LPADM was characterized by its porcelain white color, softness and good elasticity. Histological examination revealed that LPADM was totally devoid of epidermis and cellular components. On day 3 after surgery, the cavity-like structure formed by surrounding endothelial cells was observed in group L. On day 3, 5, 7 and 10 after surgery, the grafts of group L and C survived well, and new vessels were generated in group L. However, epidermal necrosis with black and blistering tissues occurred in group A, while some grafts blistering with ruddy epidermal basement were observed in group F. On day 14 after surgery, abundant blood vessels formed in group L, and all the grafts survived in group L, C and F, with the wound healing rates of (99.10±0.66)%, (99.25±0.23)%, and (97.07±1.32)%, respectively, and there was no significant difference among groups (P>0.05). The wound healing rate of group A was (27.46±2.05)%, which was significantly lower than those of the other groups (P<0.01). Conclusion The new type of micropore porcine acellular dermis matrix can be used as an ideal dermal substitute due to high survival rate when grafted with the split-thickness autograft and the ability of initiating early revascularization.

    Related Articles | Metrics
    Application of 18F-FLT and 18F-FDG in evaluation of tumor proliferation of nude mouse models of leukemia
    LU Li-yan, JIANG Lei, GAO Yun-chao, et al
       2012, 32 (10): 1312-.   DOI: 10.3969/j.issn.1674-8115.2012.10.007
    Abstract1100)      PDF(pc) (510KB)(984)       Save

    Objective To investigate the application of 18F-fluoro-3´-Deoxy-3´-L-fluorothymidine (18F-FLT) and 18F-fluorodeoxyglucose (18F-FDG) in evaluation of tumor proliferation of nude mouse models of chronic myelogenous leukemia(K562 cell lines). Methods 18F-FLT was synthesized and K562 cells were traditionally cultured. The in vitro experiment of 18F-FLT and 18F-FDG uptake assay was performed in K562 cells at 15 min, 30 min, 60 min and 120 min. After tumorbearing mouse models of leukemia were successfully established by injection of K562 cells, positron emission computed tomography (PET) was performed 30 min, 60 min and 120 min after injection of 18F-FLT and 18F-FDG. The region of interest was drawn over the tumor and background to calculate the ratio of tumor to non-tumor (T/NT). Results High-performance liquid chromatography indicated that the radiochemical purity of 18F-FLT was over 95% within 6 h. Pathological examination revealed that tumor-bearing nude mouse models of leukemia were successfully established by subcutaneous inoculation of K562 cells. A significantly higher uptake of 18F-FLT than that of 18F-FDG was observed in K562 cells in the tracer uptake experiment at various time points (P<0.05). And ratios of T/NT of 18F-FLT PET were significantly higher than those of 18F-FDG PET at different time points after injection (P<0.05). Conclusion Compared with 18F-FDG, 18F-FLT may have better performance in evaluation of tumor proliferation of nude mouse models of leukemia.

    Related Articles | Metrics
    Influence of insulin resistance on ventricular remodeling and intervention effect of rosuvastatin
    XU Wei-yuan, ZHENG Liang-rong, GUO Hang-yuan, et al
       2012, 32 (10): 1316-.   DOI: 10.3969/j.issn.1674-8115.2012.10.008
    Abstract1110)      PDF(pc) (653KB)(1030)       Save

    Objective To investigate the influence of insulin resistance on the ventricular remodeling, and to observe the intervention effect of rosuvastatin and explore its possible mechanism. Methods Forty male LDLR-/- mice aged 6 weeks were randomized into control group (NC group, fed with normal diet), high fat and high fructose diet group (HFF group, fed with normal diet+21.1% fat+20% fructose), rosuvastatin intervention group(HFFR group, intervention with rosuvastatin on the basis of that in HFF group) and rosuvastatin and mevalonic acid intervention group (HFFRMA group, fed with mevalonic acid on the basis of that in HFFR group), with 10 mice in each group. Mice were sacrificed 12 weeks after treatment, the fasting blood sugar (FBS) was determined by oxygen electrode method, fasting plasma insulin (FINS) was detected by ELISA, homeostasis model insulin resistance index (HOMA-IR) and left ventricular weight index (LVWI) were calculated, collagen volume fraction (CVF) of myocardial tissues was calculated with Picric-Sirius Red staining, and the expression of PPARα and MMP-9 protein in myocardial tissues was detected by Western blotting. Results FBS, FINS, HOMA-IR, LVWI, CVF and the expression of PPARα and MMP-9 protein in HFF group, HFFR group and HFFRMA group were significantly higher than those in NC group(P<0.05). After treatment with rosuvastatin, FBS, FINS and HOMA-IR in HFFR group and HFFRMA group were significantly improved, and LVWI, CVF and the expression of PPARα and MMP-9 protein in HFFR group and HFFRMA group were significantly lower than those in HFF group (P<0.05). However, there was no significant difference in these parameters between HFFR group and HFFRMA group (P>0.05). Conclusion Insulin resistance can be induced in LDLR-/- mice 12 weeks after high fat and high fructose diet. Insulin resistance may contribute to myocardial fibrosis and ventricular remodeling. Rosuvastatin can inhibit ventricular remodeling and improve insulin resistance, and the mechanism may be associated with the down-regulation of expression of MMP-9 and PPARα protein, which is independent of lipid-lowering effect.

    Related Articles | Metrics
    Quantitative magnetic resonance imaging analysis of parts of hippocampal volume in normal adults
    GAO Mei-chun, LU Qin-chi
       2012, 32 (10): 1321-.   DOI: 10.3969/j.issn.1674-8115.2012.10.009
    Abstract1521)      PDF(pc) (495KB)(1138)       Save

    Objective To investigate the quantitative magnetic resonance imaging (MRI) analysis of parts of hippocampal volume (HV), and to determine the normal range of HV. Methods Twenty-five subjects (13 males and 12 females) underwent a 3.0T MRI with oblique coronal thin sections (1.5 mm) oriented perpendicular to the hippocampal long axis. The hippocampus was divided into three parts of head, body and tail, and the HV including the hippocampal head volume (HHV), hippocampal body volume (HBV), hippocampal tail volume (HTV) and whole hippocampal volume (HCV) were measured on T1weighted imaging. Results The right-side HHV, HBV, HTV and HCV were (1 712.81±242.26) mm3, (1 126.59±133.10) mm3, (231.22±76.28) mm3 and (3 078.62±254.82) mm3 respectively, and the left-side ones were (1 640.86±208.70) mm3, (1 098.20±145.00) mm3, (229.52±78.39) mm3 and (2 979.69±227.25) mm3 respectively. There was no significant difference between left-side HTV and right-side HTV(P>0.05), and the right-side HHV, HBV and HCV were significantly larger than the left ones (P<0.05 for all). There was no significant difference in left-side and right-side HV between males and females (P>0.05 for both). Conclusion MRI can quantitatively analyse HV, and determine the normal range of HV in normal adults.

    Related Articles | Metrics
    Cross-sectional study on birth defects among children aged 0 to 5 years in southeastern and northeastern Chongqing
    HUANG Qiao, ZHONG Zhao-hui, LI Jie, et al
       2012, 32 (10): 1326-.   DOI: 10.3969/j.issn.1674-8115.2012.10.010
    Abstract1086)      PDF(pc) (290KB)(989)       Save

    Objective To investigate the prevalence of birth defects among children aged 0 to 5 years in southeastern and northeastern Chongqing, to illustrate the main types and conditions of defects, and to provide scientific evidence for the prevention of birth defects. Methods Subjects were selected through multi-stage proportional cluster random sampling. The criteria for diagnosis and re-checking were standardized, and the data of medical history and physical examinations were obtained by investigators with strict training. Results The prevalence rates of birth defects in southeastern and northeastern Chongqing were 39.84‰ (41/1029) and 49.48‰ (114/2190) respectively, with no significant difference between them (χ2=1.49,P=0.22). Hernia, polydactylism and cryptorchidism took the first three places of birth defects in southeastern Chongqing, while hernia, cryptorchidism and congenital heart disease in northeastern Chongqing. The prevalence rates of birth defects among boys and girls in southeastern Chongqing were 59.65‰ and 15.25‰ respectively, and those in northeastern Chongqing were 71.37‰ and 21.67‰ respectively, with the prevalence rates of boys significantly higher than those of girls in both areas (P<0.05). There was no significant difference in the prevalence rates of birth defects among different age groups in southeastern Chongqing (χ2=1.22,P=0.87). The prevalence rate of birth defects of 1-year age group was the highest in northeastern Chongqing, with that of 4-5-year age group ranking second, and there were significant differences between these two age groups (χ2=9.91,P<0.05). The prevalence rate of birth defects in the rural area (56.72‰) was higher than that of the urban area (32.02‰) in northeastern Chongqing (χ2=7.80,P<0.05), and was higher than that of the rural area in southeastern Chongqing (37.36‰)(χ2=4.50,P<0.05). Conclusion The prevalence of birth defects among children aged 0 to 5 years has been vividly illustrated by the cross-sectional investigation, which helps to identify the birth defects with high prevalence rate and severe damage and find the risk factors for the disease control and prevention.

    Related Articles | Metrics
    Surgical technique in clipping of ventral aneurysms in internal carotid artery
    FEI Zhi-min, ZHANG Jue, SHU Guo-wei, et al
       2012, 32 (10): 1330-.   DOI: 10.3969/j.issn.1674-8115.2012.10.011
    Abstract1064)      PDF(pc) (932KB)(1000)       Save

    Objective To investigate the technique in clipping of ventral aneurysms in internal carotid artery. Methods Ten cases of ventral aneurysms in internal carotid artery confirmed by 3D-CT angiography (3D-CTA) and digital subtraction angiography (DSA) were analysed. Preoperative virtual reality model of 3D-CTA, based on the body position during surgery, simulated the position of aneurysm and direction of doom and determine the existence of branching artery originating from the aneurysm or nearby parent artery. Somatosensory evoked potential monitoring, microvascular Doppler sonography, and neuroendoscopy were used before and after clipping to confirm the obliteration of aneurysms and patency of parent vessels and branching artery.If the branching artery was originating from the aneurysm, lateral side of internal carotid artery with nonring clip was recommended. Otherwise, it was often preferable to clip the aneurysm with ring or so-called fenestrated clip, whose clip-blades were placed parallel to internal carotid artery axis. If necessary, the combination use of several clips was recommended to reconstruct the parent artery. Results The scores of Glasgow Outcome Scale one month after operation were 5 points in 8 patients, 4 points in 1 patient and 3 points 1 patient. There was no case of death. Three months after operation,DSA and/or 3D-CTA revealed that there was no aneurysmal residue and no stenosis of parent and adjacent vessels. One patient suffered from permanent visual loss. Conclusion Detailed preoperative planning and proper intraoperative assisted methods and neuroendoscopy are important for clipping of ventral aneurysms in internal carotid artery.

    Related Articles | Metrics
    Study on visual P300 evoked by facial expression stimulus in patients with depression
    ZHU Yi, CHEN Xing-shi, QIU Jian-yin
       2012, 32 (10): 1334-.   DOI: 10.3969/j.issn.1674-8115.2012.10.012
    Abstract1040)      PDF(pc) (270KB)(1136)       Save

    Objective To investigate the changes of visual P300 evoked by facial expression stimulus in patients with depression. Methods Thirty patients with depression (depression group) and 30 normal controls (control group) were enrolled, and were evaluated by self-designed demographic questionnaire and Hamilton depression scale (HAMD). Besides, the examinations of visual P300 evoked by five facial expression stimulus, including depression, happiness, surprise, anger and disgust were conducted, and were compared between two groups. The correlation analysis of HAMD score with visual P300 was performed in depression group. Results The target P3 latency evoked by depression facial expression was (447.23±26.90) ms, that evoked by happiness facial expression was (444.40±28.52) ms, and both were significantly longer than those of control group (P<0.05). The HAMD score was not correlated with visual P300 evoked by facial expression stimulus in depression group (P>0.05). Conclusion There are abnormal changes of visual P300 evoked by facial expression stimulus in patients with depression.

    Related Articles | Metrics