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    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) 2019 Vol.39
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       2019, 39 (1): 0-.  
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       2019, 39 (1): 0-.  
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       2019, 39 (1): 1-.   DOI: 10.3969/j.issn.1674-8115.2019.01.001
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    Isolation and identification ofOct4 RNA-binding proteins
    MA Ji1*, GUO Chuan-liang2, 3*, FAN Shu-yue1, 3, XUE Yan1, 2, 3, ZENG Fan-yi1, 2, 3
       2019, 39 (1): 4-.   DOI: 10.3969/j.issn.1674-8115.2019.01.002
    Abstract496)      PDF(pc) (7222KB)(448)       Save
    Objective · To identify the RNA binding proteins of Oct4, a key factor of embryonic stem cells. Methods · Total RNA was extracted moembryonic stem cells (R1), which was reverse-transcribed into cDNA. Then PCR product of Oct4 mRNA were transcribed into Oct4 mRNA. Finally the candidate RNA-binding proteins were eluted applying the streptomycin affinity chromatography, and submitted for high-performance liquid chromatography combined with the mass spectrometry. The identified RNA binding proteins were further confirmedRNA immunoprecipitation (RIP). Results · 121 RNA binding proteins of Oct4 3 untranslated region (UTR) and Oct4 5 UTR were identified with liquid chromatography / mass spectrometry. There were 11 proteins with the number of peptide spectrum matches more or equal to 2, and 7 of them were selected for additional confirmation using RIP method. RIP results showed that Oct4 interacted with DSP, SOD1, LMNA, NPM1, PSIP1, NCL and HDGF. Among them, HDGF had the strongest binding ability to Oct4 mRNA. Conclusion · Identification of Oct4 RNA binding proteins will provide a theoretical basis for the regulation mechanism of Oct4, and will be a basis for the further study of its function.
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    Expression of liver-specific ZP domain-containing protein in momodels of obesity
    YUAN Ye-qing, ZHANG Ming-liang, WANG Yan-su, BAO Yu-qian
       2019, 39 (1): 11-.   DOI: 10.3969/j.issn.1674-8115.2019.01.003
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    Objective · To study the of liver-specific ZP domain-containing protein (LZP) in momodels of obesity. Methods · The gene and protein of LZP in different tissues of C57BL/6J mice were detectedrealtime-PCR and Western blotting respectively. C57BL/6J mice were treated with high fat diet (HFD) to establish the model of diet-induced obesity and ob/ob mice were also treated with HFD. The body mass and blood glucose were monitored during the experiment, then the liver weight and fat mass were measured at the end of the study. Hematoxylin-eosin staining of liver was performed to observe the morphology of liver. The of LZP in liver of model mice was also detectedrealtime-PCR and Western blotting, respectively. Results · The of LZP mRNA was mainly found in liver, while a lower gene level was also observed in several other tissues such as spleen and testisrealtime-PCR. The protein of LZP was detected in liver in C57BL/6J miceWestern blotting. Compared with normal diet group, the group treated with HFD had significantly increased body mass and total fat mass, higher blood glucose, increased liver mass and more serious hepatic steatosis (all P<0.05), while the of LZP in liver was reduced (P<0.05). Similarly, body mass and blood glucose were increased significantly in ob/ob mice (both P<0.05), though the of LZP was decreased compared with wild type littermates (P<0.05). Conclusion · Momodels of obesity display decreased of LZP in liver, indicating that LZP may play a role in metabolic homeostasis in obese individuals.
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    Comparison of the phagocytosis and inflammatory response of murine macrophages infectedLeptospirastrains 56606v and 56606a
    FAN Xia1, XIA Bi-li2, Lü Lin1, XU Meng-sha3, LI Jia-yin1, HE Ping1
       2019, 39 (1): 16-.   DOI: 10.3969/j.issn.1674-8115.2019.01.004
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    Objective &middot; To compare the response and consequence of momacrophages infectedLeptospira interrogans 56606v and 56606a, and explore the mechanisms of pathogenic Leptospira to cadisease. Methods &middot; Peritoneal macrophages of C57BL/6 mice were infectedpathogenic Leptospira 56606v and non-pathogenic Leptospira 56606a respectively. Immunofluorescence staining was performed to observe phagocytosis and clearance of Leptospira after infection, and realtime-PCR was used to determine cytokine production of macrophages. Results &middot; After 72-hour infection, strain 56606v exhibited a lower phagocytic rate but survived after incubation with peritoneal macrophages compared with strain 56606a, which showed a higher phagocytic rate but was cleared at that time point. Additionally, cytokine production of macrophages incubated with 56606v was lower than that with 56606a. Conclusion &middot; Leptospira interrogans strain 56606v can survive in macrophages, which may contribute to evasion phagocytic clearance and lead to disease, while strain 56606a can be cleared, which implicates a lower pathogenicity.
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    Role of inositol-requiring kinase 1&alpha;/X-box binding protein 1 in airway mucus secretion inducedneutrophil elastase
    LI Qi1, ZHOU Xiang-dong1, ZENG Man1, Victor P. KOLOSOV2, Juliy M. PERELMAN2
       2019, 39 (1): 21-.   DOI: 10.3969/j.issn.1674-8115.2019.01.005
    Abstract463)      PDF(pc) (8749KB)(442)       Save
    Objective · To explore the effect of endoplasmic reticulum stress (ERS) on neutrophil elastase (NE) induced mucin 5AC (MUC5AC) production in human airway epithelial cells. Methods · HBE16 airway epithelial cells were cultured and pretreated with reactive oxygen species (ROS) inhibitor N-acetylcysteine (NAC) or ERS inhibitor 4-phenylbutyrate (4-PBA), or transfected with small interfering RNA (siRNA) against inositolrequiring kinase 1α (IRE-1α) or X-box binding protein 1 (XBP-1), respectively before incubation with NE. NE group and blank control group were also set up. ROS production was assayeddetection kit; of glucose-regulated protein 78 (GRP78), phosphorylated protein kinase R-like endoplasmic reticulum kinase (pPERK), activating transcription factor 6 (ATF6), phosphorylated IRE-1α (pIRE-1α), and XBP-1 protein was detectedWestern blotting; spliced XBP-1 (XBP-1s) mRNA was measuredreal-time PCR; levels of MUC5AC protein in culture supernatant and cytoplasm were assayedELISA and immunofluorescence. Results · There was an obvious increase of ROS production with strong elevation of GRP78, ATF6, pPERK, and pIRE-1α protein in NE group cells after 24 h, compared with blank control group (P<0.05). The protein and mRNA of XBP-1s, and MUC5AC production also increased obviously (P<0.05). NAC and 4-PBA reduced ERS-related protein and MUC5AC production and secretion (P<0.05). Further studies showed that MUC5AC secretion was also bluntedIRE-1α siRNA or XBP-1 siRNA, accompanied with decreased of XBP-1s mRNA and protein (P<0.05). Conclusion · NE induces ERSproducing ROS, and increases MUC5AC protein production and secretion; IRE-1α/XBP-1 play a certain role in this process.
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    Mechanism of CCT2, a new downstream substrate of PDGFR&alpha;, on proliferation of tumor cells
    QU Guo-jun1, LU Yuan-feng2, LI Yu1
       2019, 39 (1): 28-.   DOI: 10.3969/j.issn.1674-8115.2019.01.006
    Abstract546)      PDF(pc) (6778KB)(453)       Save
    Objective &middot; To study the molecular mechanism of chaperonin containing TCP1 subunit 2(CCT2), a new downstream substrate of platelet derived growth factor receptor &alpha;(PDGFR&alpha;), in tumorigenesis. Methods &middot; Non-small cell lung cancer cell line H1703 was used. Western blotting was used to measure the phosphorylation of CCT2 upon PDGFR&alpha; inhibitor Gleevec treatment and PDGF stimulation. H1703 cells were divided into siCon group, siPDGFR&alpha; group and siCCT2 group; 48 h later, cell number counting was used to test the effect of CCT2 on cell growth after siRNA transfection. H1703 cells were divided into siCon group, siPDGFR&alpha; group, siAKT group and siCCT2 group; Western blotting was used to measure the protein level of PDGFR&alpha; and PARP. Cell fractionation was used to detect the cellular localization of CCT2 and co-immunoprecipitation was used to test the interaction between CCT2 and PDGFR&alpha;. Results &middot; CCT2 phosphorylation was inhibitedGleevec and inducedPDGF. Compared to the control group, the number of cells transfectedsiCCT2 reduced30% (P0.006). The protein level of PDGFR&alpha; was also decreased in siCCT2 transfected cells, whereas the cleavage of PARP was increased. CCT2 was localized in both cytoplasmic and membrane fractions and interacted with PDGFR&alpha; directly. Conclusion &middot; CCT2 is a new downstream substrate of PDGFR&alpha;. CCT2 can promote tumor cells growthinteracting and stabilizing PDGFR&alpha;.
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    Screening of the anti-atherogenic drugsusing THP-1-HIF-1&alpha;-HER-Luciferase cell model
    QIAN Yu-jun1, QIN Chun-xia1, SUN Li-li1, DING Hua-min1, LI Tie-jun1, 2
       2019, 39 (1): 33-.   DOI: 10.3969/j.issn.1674-8115.2019.01.007
    Abstract496)      PDF(pc) (7721KB)(415)       Save
    Objective · To screen the anti-atherosclerosis (AS) activity of the compoundsusing THP-1-HIF-1α-HER-Luciferase high-throughput model, and to verify the anti-AS function of the effective compounds. Methods · THP-1-HIF-1α-HER-Luciferase cells were pretreated with different concentrations of compounds (1, 10, and 100 μg/mL) for 2 h, then cultured under hypoxia for 24 h. Luciferase activity of cells was detected and compounds with anti-AS activity were screenedluciferase activity evaluation. THP-1 and U937 cells were pretreated with effective compounds, and then induced for 24 hoxidized low density lipoprotein (OX-LDL). The formation of foam cells was observedoil red staining. The mRNA level of hypoxia-inducible factor 1α (HIF-1α) was detectedreal-time quantitative PCR (qPCR). HIF-1α protein was detectedWestern blotting. Anti-AS activity of effective compounds were evaluated. Results · Among the 200 compounds, 11 compounds could significantly inhibit the increase of luciferase activity in THP-1-HIF-1α-HER-Luciferase cells inducedhypoxia (all P<0.05), and compound numbered 14 (C14) had the most significant inhibitory effect. THP-1 and U937 cells formed foam cells induced for 24 hOX-LDL. However, cells were pretreated with C14 for 2 h, which could significantly inhibit the formation of foam cells inducedOX-LDL. Cells were induced for 24 hOX-LDL, which could significantly increase the of HIF-1α mRNA and protein (all P<0.05), while cells pretreated with C14 could significantly inhibit the increase of HIF-1α mRNA and protein in a gradient-dependent manner (all P<0.05). Conclusion · THP-1-HIF-1α-HER-Luciferase high-throughput model can be reliability used in screening of compounds with anti-AS activity. C14 has the good anti-AS activity characteristics.
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    Composition differences of polyphenolic compounds in fruits of Lycium ruthenicum Murr. and Lycium barbarumL.
    WANG Qi1, TANG Hui-ru2
       2019, 39 (1): 39-.   DOI: 10.3969/j.issn.1674-8115.2019.01.008
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    Objective &middot; Both Lycium barbarum L. (LB) and Lycium ruthenicum Murr. (LR) belong to the Lycium genus of Solanaceae family but their fruits have significant phenotypic differences in terms of color and shapes. This study is aimed to investigate the inter-species difference of their fruit polyphenol composition. Methods &middot; The polyphenol composition of fresh and dried fruits of two Lycium species were comprehensively analyzed using ultra-high performance liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry (UHPLC-ESIQ-TOF-MS). Results &middot; 35 polyphenolic compounds were detected and quantified in the fresh and dried Lycium fruits including phenolic acids, anthocyanins and phenolamides (i.e., the amide-adducts of polyphenolic acids and polyamines). For both species, the dried fruits had more polyphenolic compounds than their fresh fruits with significant inter-species difference; for both fresh and dried fruits, LR had more polyphenolic compounds than LB. Conclusion &middot; There are significant inter-species differences between these two Lycium in both fresh and dried fruits. These results offer essential information for further understanding of the biological functions of these two Lycium fruits as phytomedicines and functional food.
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    Association analysis of FOS gene rs1063169 polymorphism and Alzheimers disease in Han Chinese people
    CHEN Yan1, FANG Xin-yu1, WANG Ye-wei1, NI Jian-liang2, ZHANG Jiang-tao2, LU Wei-hong1, LI Tao3, ZHANG Deng-feng4, ZHANG Chen1
       2019, 39 (1): 47-.   DOI: 10.3969/j.issn.1674-8115.2019.01.009
    Abstract471)      PDF(pc) (6609KB)(481)       Save
    Objective · To investigate the relation between FOS gene and Alzheimers disease (AD), and the relation between tag single nucleotide polymorphism (SNP) in rs1063169 and AD in Han Chinese people. Methods · The difference in FOS gene in various brain regions between AD patients and healthy people was studied using Alzdata website, and protein-protein interaction (PPI) network among FOS protein and 28 proteins encodedAD-related susceptibility genes was constructed through String database into explore whether FOS gene was associated with AD in these known database. Then, 715 AD patients and 760 healthy controls Southwest and East China were collected to analyze tag SNP rs1063169SNaPshot assay. Results · The cross-platform normalized data on Alzdata showed that FOS gene in AD patients expressed differently in entorhinal cortex (P0.003) and hippocampus (P0.001) compared with healthy people. PPI network found out that FOS protein had interactions with 4 proteins encodedAD-related susceptibility genes, which were apolipoprotein E (APOE), clusterin (CLU), β-amyloid precursor protein (APP), and protein-tyrosine kinase 2β (PTK2B). However, there was no significant difference in the frequencies of genotypes and alleles in rs1063169 between the AD cases and the healthy controls (P>0.05). Conclusion · There is differential of FOS in the entorhinal cortex and hippocampus of AD patients and healthy people, and FOS protein may have effects on the development of AD through the interaction with proteins encodedAD-related susceptibility genes, but no relation was found between rs1063169 polymorphism and AD in the collected Han Chinese people.
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    Construction of point-based mandibular median sagittal plane
    ZHANG Xin1, 2, LIU Yang1, 2, 3, ZHOU Jian-ping1, 2, 3, WU Yan1, 2, 3, ZHENG Lei-lei1, 2, 3, DAI Hong-wei1, 2, 3
       2019, 39 (1): 52-.   DOI: 10.3969/j.issn.1674-8115.2019.01.010
    Abstract435)      PDF(pc) (10142KB)(449)       Save
    Objective · To develop a new point-based mandibular median sagittal plane (MMSP)selecting varied mandibular planes which were formedcombining bony landmarks derived multiple regions of the mandible. Methods · Forty adult patients were evenly divided into two groupsthe degree of chin deviation: mandibular asymmetric group (MA group) and mandibular symmetric group (MS group). Three dimensional (3D) mandibular models were rebuiltMimics 19.0 software. In this software, 3D coordinates of 4 central landmarks and 6 pairs of lateral landmarks were collected. Then 120 candidate planes were constructedpoint-based method, and compared with the reference plane formedthe iterative closest point (ICP) algorithm. All the planes were reordered and screenedgeometric indices such as angle, distance and area in MS group. In addition, the clinical symmetrical indices such as point-to-plane distance, surface area difference, volume difference, and 3D deviation ratio of screened planes were compared in MA and MS groups. Results · The candidate planes co-constructed with landmarks three mandibular regions (symphysis, mandibular angle and coronoid process) were the top 5% of all the planes. Among them, no statistical difference between B-Cor-Golat plane (constructed with supramentale, coronoid superius and gonion lateralis) and ICP plane was found (P >0.05), while there were significant differences between the rest screened planes and the reference plane (P<0.05). Conclusion · The B-Cor-Golat plane can be used as the mandibular median sagittal plane to reflect the symmetrical degree of the mandible well.
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    Analysis of biomarkers for predicting left ventricular remodeling after successful revascularization of acute myocardial infarction
    TAO Yi-jing1*, XIA Zhi-li1*, GAO Cheng-jie2, GAO Ya-jie1, WU Hao3, WAN Qing1, LI Yong-guang1, LU Zhi-gang1, SHEN Cheng-xing1, PAN Jing-wei1
       2019, 39 (1): 60-.   DOI: 10.3969/j.issn.1674-8115.2019.01.011
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    Objective · To investigate the correlation between the biomarkers and left ventricular remodeling (LVR) in patients after acute myocardial infarction (AMI). Methods · 220 patients with AMI who were admitted in the Department of Cardiology of the Shanghai Sixth Peoples Hospital January 1st, 2015 to January 1st, 2016 and received successful primary percutaneous coronary intervention were included in this study sequentially. AMI patients due to right coronary artery were excluded. LVR after AMI was defined as more than 20% increase of the left ventricular end diastolic volume (LVEDV) measuredechocardiogram in 1-year-follow-up compared with LVEDV at admission. Patients were divided into LVR group and non-LVR group. The differences of myocardial injury markers and inflammatory factors between the two groups were compared, and the correlation between LVR and the biomarkers was analyzed. Results · Compared with the non-LVR group, the cTnI (serum cardiac troponin I), CKMB (creatine kinase MB) and myoglobin levels at admission and peaks, as well as neutrophil to monocyte ratio and neutrophil to lymphocyte ratio (NLR) in the LVR group were increased significantly (all P<0.05). There was no significant difference in proBNP (brain natriuretic peptide precursor) and BNP levels between the two groups. Logistic regression analysis showed that cTnI and NLR at admission were correlated well with LVR. ROC curve analysis showed that the area under the cTnIadmission curve (AUC) was 0.704, and the sensitivity and specificity of cTnIadmission to predict LVR after AMI were 69.2% and 64.3% respectively, when 9.14 μg/L was chosen as the cut-off point. The area under the NLR curve (AUC) was 0.664, and the sensitivity and specificity of NLR were 70.6% and 60.2% respectively, when 5.87% was chosen as the cut-off point. Conclusion · The increased levels of cTnI and NLR at admission in patients with AMI are independent predictors of LVR.
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    Relationship between glomerular filtration rate and acute ischemic stroke in middle-aged and elderly population
    ZHANG Cui-ping, OUYANG Xiao-chun, YU Xiao-li, XIONG Wen-juan, WANG Yan-qiu, MA Yao
       2019, 39 (1): 65-.   DOI: 10.3969/j.issn.1674-8115.2019.01.012
    Abstract478)      PDF(pc) (6410KB)(446)       Save
    Objective · To explore the occurrence risk and clinical significance of glomerular filtration rate (GFR) level and acute ischemic stroke in middle-aged and elderly population. Methods · The clinical data of 292 hospitalized patients in the Department of Neurology at the No.908 Hospital of the Peoples Liberation Army Joint Logistics Support Force Jan. 2016 to Jun. 2018 were retrospectively analyzed, including gender, age, diastolic blood pressure, body mass index, smoking and drinking history, erythrocyte count, brain images, and the level of blood glucose, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, total cholesterol, GFR, blood urea nitrogen, blood uric acid, serum creatinine, glutamic-oxaloacetic transaminase, homocysteine. According to the GFR level, patients were divided into normal GFR group and low GFR group. The clinical characteristics were compared between two groups. Multivariate Logistic regression analysis was used to investigate the relationship between GFR level and the occurrence of acute ischemic stroke. Results · The number of patients in normal GFR group and low GFR group was 154 (52.74%) and 138 (47.26%), respectively. Chi-square test or t test analysis showed that there was no significant difference in gender, body mass index, smoking history, drinking history, erythrocyte count, blood glucose, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, total cholesterol, glutamic-oxaloacetic transaminase and homocysteine between two groups, and significant difference in age, diastolic blood pressure, GFR, blood urea nitrogen, blood uric acid, serum creatinine (all P<0.05). The incidence rate of acute ischemic stroke in normal GFR group and low GFR group was 41.56% (64/154) and 59.42% (82/138), respectively (χ29.291, P0.002). Compared with the normal GFR group, the occurrence risk OR (95% CI) of acute ischemic stroke in lower GFR group was 2.06 (1.29–3.29) (P0.002) and 2.04 (1.01–4.12) (P0.047) before and after adjusted the related risk factors. Conclusion · The low GFR levels are associated with the occurrence of acute ischemic stroke in middle-aged and elderly population.
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    Establishment of risk prediction model for pulmonary complications in elderly patients with multiple rib fractures
    MAO Yi-Ming1, WU Chang-Jiang2
       2019, 39 (1): 69-.   DOI: 10.3969/j.issn.1674-8115.2019.01.013
    Abstract477)      PDF(pc) (5901KB)(417)       Save
    Objective · To establish a risk prediction model for pulmonary complications in elderly patients with multiple rib fractures. Methods · A total of 150 cases of elderly patients with multiple rib fractures were analyzed retrospectively January 2016 to December 2017 in the Department of Thoracic Surgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong University School of Medicine. Patients were divided into case group (n88) and control group (n62) according to whether pulmonary complications occurred. Univariate analysis and Logistic regression analysis were performed based on 8 factors, including gender, age, basic pulmonary disease, smoking history, the number of rib fractures, expectoration, diabetes, and the treatment time. Results · The results of univariate analysis showed that age, basic pulmonary disease, smoking history, the number of rib fractures, expectoration, diabetes, and the treatment time were associated with pulmonary complications (P<0.05). Logistic regression analysis showed that basic pulmonary disease (OR3.338, 95% CI 2.772-11.606, P0.003), smoking history (OR2.407, 95% CI 1.103-5.253, P0.027), the number of rib fractures (OR3.321, 95% CI 1.178-9.359, P0.023), and expectoration (OR4.034, 95% CI 1.818-8.952, P0.001) were the independent risk factors for pulmonary complications in elderly patients with multiple rib fractures. The area under the model ROC curve was 0.816, and the regression model was reliable. Conclusion · Basic pulmonary disease, smoking history, the number of rib fractures and expectoration are the independent risk factors for pulmonary complications in elderly patients with multiple rib fractures. More attention should be paid to these factors into reduce the incidence of pulmonary complications.
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    Effect of acute hypervolemic fluid infusion during anesthesia induction on intraoperative hemodynamics in the patients undergoing Da Vinci robot-assisted pancreatic surgery
    QIAN Yang, LUO Yan
       2019, 39 (1): 73-.   DOI: 10.3969/j.issn.1674-8115.2019.01.014
    Abstract587)      PDF(pc) (8128KB)(455)       Save
    Objective · To investigate the effect of acute hypervolemic fluid infusion (AHFI) during induction of general anesthesia on intraoperative hemodynamic fluctuation in the patients undergoing pancreatic robotic surgery. Methods · Sixty patients undergoing Da Vinci robot-assisted pancreatic surgery were randomly divided into routine infusion group (control group, n30) and AHFI group (n30). In AHFI group, the patients were infused with 12.5 mL/kg crystalloid solution before anesthesia induction, 12.5 mL/kg colloidal solution before pneumoperitoneum, and 8 mL/(kg·h) (crystalloid solution:colloidal solution1:1) continuously after pneumoperitoneum. The patients in control group were treated with routine liquid therapy. The mean arterial pressure (MAP), heart rate (HR), central venous pressure (CVP), cardiac index (CI), stroke volume variation (SVV), and the incidence of adverse events in both groups were recorded. After operation, the exhaust time, hospitalization time and 3-month mortality of the two groups were followed up. Results · Compared with control group, the consumption of crystalloid solution, colloidal solution and both solutions in AHFI group increased (P<0.05), while the incidence of adverse events was less (P0.020). There was no significant difference in the MAP variation, HR variation and CI between the two groups (P>0.05). Compared with control group, CVP increased significantly and SVV decreased significantly in AHFI group before pneumoperitoneum, after pneumoperitoneum and after postural changes (P<0.05). There was no significant difference in the exhaust time, hospitalization time and 3-month mortality between the two groups (P>0.05). Conclusion · Compared with conventional fluid infusion, AHFI during anesthesia induction can reduce the occurrence of adverse events in the patients undergoing Da Vinci robot-assisted pancreatic surgery. There is no significant difference in the recovery of gastrointestinal function and prognosis between the two methods.
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    Application of optical coherence tomography angiography in children with anisometropic amblyopia
    XIANG Xiao-qiong, LUO Li-ying, TANG Min, FU-Yang
       2019, 39 (1): 79-.   DOI: 10.3969/j.issn.1674-8115.2019.01.015
    Abstract472)      PDF(pc) (7765KB)(402)       Save
    Objective &middot; To explore the application value of optical coherence tomography angiography (OCTA) in retinal microvascular blood flow in children with anisometropic amblyopia. Methods &middot; 31 children with monocular anisometropic amblyopia in the Department of Ophthalmology in Shanghai General Hospital, Shanghai Jiao Tong University June to August in 2018 were included. The 3 mm &times; 3 mm and 6 mm &times; 6 mm area centered on the macular area and 4.5 mm &times; 4.5 mm area centered on the optic disc area were detectedOCTA. The software automatically calculated the average blood flow densities of superficial capillary plexus (SCP) and deep capillary plexus (DCP), and the average blood flow densities of optic disc and the peripapillary area, the average area of foveal avascular zone (FAZ), as well as the average foveal thickness in the anisometropic amblyopia and the contralateral eyes. The anisometropic amblyopia eyes of the children were classified as the amblyopia group, and the contralateral non-ambly eyes were classified as the contralateral eye group. The differences in blood flow parameters between two groups were compared. Results &middot; In the 3 mm &times; 3 mm macular scan, the average blood flow densities of SCP and DCP in the amblyopia group were (46.40&plusmn;4.72)% and (52.17&plusmn;2.82)%, respectively, and the average blood flow densities in the contralateral eye group were (48.48&plusmn;3.46)% and (54.31&plusmn;2.18)%, respectively. The differences in the average blood flow densities of SCP and DCP between two groups were statistically significant (P0.012, P0.012). In the 6 mm &times; 6 mm macular scan, the average blood flow densities of SCP and DCP in the amblyopia group were (47.41&plusmn;3.04)% and (48.92&plusmn;5.34)%, respectively, and the average blood flow densities in the contralateral eye group were (50.36&plusmn;2.70)% and (51.54&plusmn;4.69)%, respectively. The differences between two groups were statistically significant (P0.016, P0.046). In the 4.5 mm &times; 4.5 mm optic disc scan, the average blood flow densities of the optic disc in the amblyopia group and the contralateral eye group were (48.98&plusmn;4.03)% and (52.06&plusmn;3.90)%, respectively. The difference was also statistically significant (P 0.040). The average blood flow densities of the peripapillary area in the amblyopia group and the contralateral eye group were (52.16&plusmn;2.22)% and (52.44&plusmn;1.50)%, respectively. The difference was not statistically significant. Conclusion &middot; The average blood flow densities of SCP, DCP and optic disc in the anisometropic amblyopia eyes are significantly lower than that in the contralateral eyes. OCTA has certain application value in evaluating retinal blood flow in children with anisometropic amblyopia.
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    Reliability and validity of defeat scale on anxiety and depression in medical students
    TANG Hua1, WANG Su-ping1, 2, GONG Rui-jie3, WANG Ze-zhou2, CAI Yong2
       2019, 39 (1): 84-.   DOI: 10.3969/j.issn.1674-8115.2019.01.016
    Abstract552)      PDF(pc) (6403KB)(513)       Save
    Objective &middot; To introduce the defeat scale (DS) and evaluate its reliability and validity among Chinese junior medical students. Methods &middot; With convenience sample method, 665 medical students were examinedthe Chinese version of DS, and 29 of them were retested one month later. The structural validity, criterion validity, and content validity of DS were accessedfactor analysis and correlation analysis. The reliability of DS was evaluatedinternal consistency reliability (Cronbachs &alpha; coefficient), split-half reliability (Spearman-Brown coefficient), and retest reliability. Results &middot; The exploratory factor analysis showed that two factors including decadence (13 items) and low sense of achievement (3 items) could be extracted. Confirmatory factor analysis showed that model revisedtwo factors fitted well (&chi;2/df7.199, RMSEA0.097, CFI0.925). The correlation coefficient between each item of content validity and its sub-dimension were among 0.678-0.889. The correlation coefficient was 0.738 between DS and patients health questionnaire depression scale-9 item, and -0.712 between DS and Rosenberg self-esteem scale. The Cronbachs &alpha; coefficient of DS was 0.933, the Spearman-Brown coefficient was 0.925, and the retest reliability was higher than 0.900. Conclusion &middot; The DS has good validity and reliability among Chinese junior medical students.
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    Co-occurring psychosocial problems and consistent condom among unmarried female miworkers of two factories in Shanghai
    SHEN Qiu-ming1, SHI Yue1, WANG Ze-zhou1, ZHANG Shu-xian1, Lhakpa Tsamlag1, CAI Bo2, CAI Yong1
       2019, 39 (1): 89-.   DOI: 10.3969/j.issn.1674-8115.2019.01.017
    Abstract438)      PDF(pc) (8552KB)(412)       Save
    Objective &middot; To investigate condom among unmarried female miworkers in Shanghai and explore its relation with some psychosocial problems. Methods &middot; A questionnaire survey was conductedcluster random sampling. A total of 903 unmarried female miworkers who had sexual experience were enrolled to evaluate their condom and psychosocial factors. Logistic regression was used to analyze the related factors of condom use. Results &middot; Among the unmarried female miworkers, the percentage of consistent condom was only 13.8%. Univariate Logistic regression showed that low self-esteem (OR0.47, 95% CI0.25-0.90) and depression (OR0.59, 95% CI0.38-0.93) were related to inconsistent condom after correcting demographic factors. Multivariate Logistic regression showed that depression (OR0.58, 95% CI0.37-0.90) was the main factor related to inconsistent condom use. The participants with at least two psychosocial problems were more difficult to condoms consistently (OR0.56, 95% CI0.32-0.97). Conclusion &middot; The unmarried female miworkers with depression or co-occurring psychological problems in Shanghai are more likely to have unsafe sex behavior, which indicates the importance of psychological interventions.
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    Role of On-cells and Off-cells in the rostral ventromedial medulla in pain modulation
    WANG Ruo-xi, JIANG Tao, JIAO Ying-fu, GAO Po, YANG Li-qun, YU Wei-feng
       2019, 39 (1): 95-.   DOI: 10.3969/j.issn.1674-8115.2019.01.018
    Abstract562)      PDF(pc) (6653KB)(490)       Save
    The rostral ventromedial medulla is one of the key structures in descending pain modulation system. It receives inputs the thalamus, the periaqueductal gray, and parabrachial nucleus, and sends descending projections through the dorsolateral funiculus and ventrolateral funiculus to the spinal dorsal horns. The rostral ventromedial medulla is thought to be the final relay in descending modulation of pain. The neurons in this region can be classified into On-cells, Off-cells and Neutral cells according to the changes in the firing activity before tail flick. This review mainly focuses on pain modulation functions and potential analgesia mechanisms of On-cells and Off-cells.
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    Research progress on esophageal stents for children
    HU Han-bo, SUN Kun
       2019, 39 (1): 99-.   DOI: 10.3969/j.issn.1674-8115.2019.01.019
    Abstract493)      PDF(pc) (5429KB)(330)       Save
    Currently, benign esophageal stricture is a common clinical esophageal disease in children, and its common treatment is balloon dilatation. According to the complexity of the lesion, the child often suffers multiple esophageal dilation. Besides, esophageal stent provides a new choice for the treatment of esophageal stricture in children becaof the long-lasting support. However, the complications limit its clinical application, such as chest pain, restenosis, and stent displacement. This article reviewed the current research and clinical application of esophageal stents for children.
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    Percutaneous mechanical thrombectomy combined with catheter-directed thrombolysis in the treatment of deep venous thrombosis of lower extremity via contralateral femoral vein approach
    LU Hua-xiang, HUANG Jia-qi, HUANG Sheng, YANG Guang-lin, LIU Xiao-bing, LIU Guang
       2019, 39 (1): 102-.   DOI: 10.3969/j.issn.1674-8115.2019.01.020
    Abstract532)      PDF(pc) (5610KB)(238)       Save
    Objective &middot; To analyze the early clinical effects of acute deep venous thrombosis (DVT) of the lower extremities through a contralateral femoral vein approachpercutaneous mechanical thrombectomy (PMT) combined with catheter-directed thrombolysis (CDT) therapy. Methods &middot; A retrospective analysis about 45 patients (September 2016 to August 2017) was conducted to analyze the success rate of the technique, the degree of thrombolysis in different venous segments, and the incidence of complications during the treatment of PMT+CDT. Results &middot; Technique successful rate was 100%. 86.7% of lower extremity thrombosis (LET) Ⅰ segments (calf veins), 82.2% of LET Ⅱ segments (femoral and popliteal veins) and 71.1% of LET Ⅲ segments (common iliac and femoral veins) were completely dissolved. No serious complications occurred. Conclusion &middot; It is feasible, safe and effective to PMT+CDT in acute DVT.
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    Diagnosis and treatment of a case of pulmonary embolism with normal D-dimer
    ZHANG Yi-qin1, XIE Song-ming2, WANG Wen-an1
       2019, 39 (1): 106-.   DOI: 10.3969/j.issn.1674-8115.2019.01.021
    Abstract590)      PDF(pc) (4380KB)(317)       Save
    The patient was a 51-year-old female. She was admitted to the hospital becaof &ldquo;repetitive convulsions for 1 month&rdquo;. No significant neurological positive signs were found in the examination. D-dimer was in the normal range in several times of detection. Brain CT and electroencephalograph showed no obvious abnormality. Sustained hypoxemia occurred on admission, and CT imaging of pulmonary artery perfusion was performed. In the CT images bilateral distal main pulmonary arteries and their main branches showed vessel filling defect, partial lumen stenosis and occlusion, and bilateral small branches of pulmonary arteries showed extensive similar filling defect. Finally the patient was diagnosed with pulmonary embolism, whose condition was safter anticoagulation therapy with warfarin.
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       2019, 39 (10): 1-.  
    Abstract230)      PDF(pc) (2288KB)(266)       Save
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       2019, 39 (10): 2-.  
    Abstract172)      PDF(pc) (13162KB)(184)       Save
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       2019, 39 (10): 3-.  
    Abstract176)      PDF(pc) (15438KB)(326)       Save
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    Metabolic imaging reveals a unique preference of symmetric cell division and homing of leukemia-initiating cells in an endosteal niche
    The research group of ZHENG Jun-ke
       2019, 39 (10): 1107-.   DOI: 10.3969/j.issn.1674-8115.2019.10.001
    Abstract520)      PDF(pc) (3677KB)(186)       Save
    The metabolic properties of leukemia-initiating cells (LICs) in distinct bone marrow niches and their relationships to cell-fate determinations remain largely unknown. Using a metabolic imaging system with a highly responsive genetically encoded metabolic sensor, SoNar, we reveal that SoNar-high cells are more glycolytic, enriched for higher LIC frequency, and develop leukemia much faster than SoNar-low counterparts in an MLL-AF9-induced murine acute myeloid leukemia model. SoNar-high cells mainly home to and locate in the hypoxic endosteal niche and maintain their activities through efficient symmetric division. SoNar can indicate the dynamics of metabolic changes of LICs in the endosteal niche. SoNar-high human leukemia cells or primary samples have enhanced clonogenic capacities in vitro or leukemogenesis in vivo. PDK2 fine-tunes glycolysis, homing, and symmetric division of LICs. These findings provide a unique angle for the study of metabolisms in stem cells, and may lead to development of novel strategies for cancer treatment.
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    Intra-bone marrow injection of fetal liver mononuclear cells to reconstruct the hematopoietic system in mice
    YI Qing-fu1, CHI Ya-nan1, LIU Guang-hui2, 3, YANG Guan-heng2, 3, GONG Xiu-li2, 3, FAN Shu-yue1, ZENG Fan-yi1, 2, 3
       2019, 39 (10): 1110-.   DOI: 10.3969/j.issn.1674-8115.2019.10.002
    Abstract426)      PDF(pc) (7367KB)(223)       Save
    Objective &middot; To observe the effect of intra-bone marrow injection (IBMI) of fetal liver mononuclear cell suspension on reconstructing the hematopoietic system in mice. Methods &middot; The fetal liver mononuclear cells the mice with gestational age of 13.5 days were obtainedFicoll separation technique, and then the hematopoietic stem cells (HSCs) were obtainedmagnetic bead sorting. The fetal liver mononuclear cells were injected into the tibias of 137Cs irradiated miceIBMI. The recipient mice were observed for reconstitution of the hematopoietic systemroutine blood examination, blood smear and bone marrow smear. The donor HSCs in the bone marrow of the recipient mice was subjected to flow cytometry 1 year after transplantation. Results &middot; HSCs accounted for 0.171% in the mofetal liver mononuclear cells. One week after transplantation of mofetal liver mononuclear cells, blood cells derived donor cells were detected peripheral blood; in the third week after transplantation, peripheral blood leukocytes, red blood cells and hemoglobin of recipient mice were restored to the pre-irradiation levels; the percentage of donor-derived blood cells in peripheral blood in the fifth week was stable; the HSCs in the bone marrow of recipient mice were derived donor cells 1 year after transplantation. Conclusion &middot; The murine fetal liver mononuclear cells can efficiently reconstruct the hematopoietic system of miceIBMI.
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    Protective effect of recombinant human IL-1Ra on hepatocytes in vitro
    ZHENG Ying, XIAO Xin-yi, YANG Zhuo-yi, ZHOU Mei-qi, CHEN Hui, YUAN Yun-sheng
       2019, 39 (10): 1115-.   DOI: 10.3969/j.issn.1674-8115.2019.10.003
    Abstract399)      PDF(pc) (7379KB)(187)       Save
    Objective &middot; To evaluate protective effects of recombinant human IL-1Ra (rhIL-1Ra) on acute liver injury in vitrousing D-galactosamine (D-GalN) and HepG2 cells to establish the D-galactosamine (D-GalN)-induced HepG2 cells injury models. Methods &middot; Models of HepG2 cells injury inducedD-GalN was established. HepG2 cells were maintained in mediums which contained different concentration of D-GalN (0.02, 0.2, 2 or 4 mg/mL) for different time (1, 2 or 3 d). Optimized concentration and time of D-GalN were used to analyze cell viability and morphology. A serial dose of rhIL-1Ra (10, 20 or 50 &mu;g/mL) was used to treat HepG2 cells which were challenged with D-GalN. Cell apoptosis and the levels of intracellular reactive oxygen species (ROS) were analyzed in different treatment groups. Real-time PCR was employed to analyze the mRNA levels of IL-1&beta;, IL-6 and TNF-&alpha; in cells. ERK1/2 inhibitor (SCH772984) was used to confirm whether ERK1/2 phosphorylation played a critical role in IL-1Ra protecting hepatocytes or not. Results &middot; Cell viability was significantly decreasedD-GalN whose concentration was 4 mg/mL in HepG2 cells after 2 d. Compared with the control group, rhIL-1Ra could significantly improve cell survival and down-regulate the level of ROS in the cells. RhIL-Ra also could suppress of pro-apoptotic cytokines factors (IL-1&beta;, IL-6 and TNF-&alpha;) inducedD-GalN in HepG2 cells. The results also showed that erk1/2 signaling pathways have certain effect on mediating the injury of rhIL-1Ra to protect hepatocytes. Conclusion &middot; RhIL-1Ra can protect hepatocytes toxinsdirectly targeting hepatocytes and inhibit cells apoptosisactivating ERK1/2 pathway in HepG2 cells.
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    Effect of endothelial dysfunction in hypertrophic scars on fibroblasts biology
    XI Jing, ZHENG Jie-xin, WANG Xi-qiao
       2019, 39 (10): 1122-.   DOI: 10.3969/j.issn.1674-8115.2019.10.004
    Abstract390)      PDF(pc) (7108KB)(169)       Save
    Objective &middot; To investigate the effect of endothelial dysfunction in hypertrophic scar regression on the fibroblasts biology. Methods &middot; Scar-derived endothelial cells were isolated and cultured proliferative scars, regressive scars and mature scars, and the endothelial cells normal skin as control. After 6 h of culture, the endothelial cell culture medium (ECCM) was harvested. In addition, the fibroblasts normal skin were cultured and treated with the ECCM. After 48 h, the cell viability, total collagen production, and cell apoptosis were assessedAlamar blue, sirius red staining, and TUNEL assay, respectively. Furthermore, anti-VEGF (vascular endothelial growth factor), anti-PDGF (platelet derived growth factor), anti-TGF-&beta;1 (transforming growth factor-&beta;1), anti-ET-1 (endothelin 1), and anti-bFGF (basic fibroblast growth factor) neutralizing antibodies were individually added to the ECCM to identify their effects on fibroblasts biology. Results &middot; The results revealed that the ECCM regressive scars inhibited fibroblasts viability and collagen production, and induced apoptosis (PPPConclusion &middot; Vascular endothelial dysfunction in hypertrophic scars can inhibit fibroblast proliferation and collagen production, and induce cell apoptosis, especially in regressive scars. TGF-&beta;1, PDGF and bFGF may play a major role in this process.
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    Microchip based on 3D printing technology for simulating intestinal villi and tumor surface topography
    ZHANG Yi-heng1, DU Jing2, 3, XIA An-yue1, CUI Jin-hui2, GAN Ming-zhe2, LIU Pei-feng1
       2019, 39 (10): 1127-.   DOI: 10.3969/j.issn.1674-8115.2019.10.005
    Abstract408)      PDF(pc) (8610KB)(202)       Save
    Objective · To propose a method for making organ-on-a-chip based on 3D printing, and study the relationship between cell growth on the chips and various factors. Methods · Through 3D printing technology and surface microstructure transfer method, ulcer-like and ridge-like microstructures of the tumor surface and the intestinal villi were fabricated on a polydimethylsiloxane (PDMS) chip. Combined with fluorescence imaging, the effects of surface modification, shapes and heights of microstructures, and culture time on the surface coverage and density of Caco-2 cells on the chip were measured. Results · The PDMS chip was more likely to induce cell adhesion and growth rather than the 3D printing resin chip. On the surface of three-dimensional structure, cell surface coverage and cell density increased after the surface was treated with rat tail collagen Ⅰ (PP0.000). After surface modification, there was no significant difference in cell density at the same height of different steric structure (P>0.05). Conclusion · The intestinal villi and tumor topological organ chips can be fabricated3D printing technology and surface microstructure transfer method. The surface modification and microstructure height affect the cell growth on the surface.
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    Effects and mechanisms of glutamic-pyruvic transaminase 2 on cisplatin resistance in gastric cancer
    YUAN Yuan, MEI Jun
       2019, 39 (10): 1134-.   DOI: 10.3969/j.issn.1674-8115.2019.10.006
    Abstract369)      PDF(pc) (10159KB)(199)       Save
    Objective &middot; To investigate the role and mechanism of glutamic-pyruvic transaminase 2 (GPT2) on cisplatin resistance in gastric cancer. Methods &middot; The Kaplan Meier-Plotter database was used to analyze the relationship between GPT2 and poor prognosis of gastric cancer. The s of GPT2 in gastric cancer cells and tissues were detectedquantitative real-time PCR (qPCR), Western blotting and immunohistochemistry (IHC). The cytotoxicity of cisplatin at different concentrations to human gastric cancer cells and normal gastric epithelial cells was detectedCCK-8 assay. GPT2 over and knockdown cell lines were constructed in cisplatin sensitive MKN28 cells and insensitive MKN45 cells, respectively. CCK-8 assay, colony formation assay and Western blotting were performed to evaluate the cellular cytotoxicity, stemness of cancer cells and the changes of key proteins in stemness-related signaling pathways in GPT2 over and knockdown cell lines, respectively. Results &middot; The high of GPT2 was negatively correlated with the survival of gastric cancer patients. Gastric cancer cells with high of GPT2 were resistant to cisplatin, while cells with low of GPT2 were sensitive to cisplatin. Over of GPT2 could decrease the cell sensitivity to cisplatin, nevertheless knockdown of GPT2 could increase the cell sensitivity to cisplatin. Meanwhile, the further study revealed that over of GPT2 could activate the extracellular regulated protein kinases (ERK) signaling pathway, up-regulate the of SRY-box 2 (SOX2) and Nanog homeobox (NANOG), and enhance the ability of colony formation, while knockdown of GPT2 could inhibit ERK signaling pathway, reduce the of SOX2 and NANOG, and suppress the ability of colony formation. Conclusion &middot; GPT2 are related to the sensitivity of cisplatin treatment. Over of GPT2 can increase the resistance of gastric cancer to cisplatin treatmentactivating ERK signaling pathway and up-regulating the of SOX2 and NANOG.
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    Construction of scell lines expressing large conductance Ca2+-activated K+ channel &alpha;-subunit and molecular mechanism of potassium excretion in this channel
    YING Si-qi, ZHANG Ya, GUO Qin, YANG Yang, LIU Shuang, ZHANG Chong
       2019, 39 (10): 1142-.   DOI: 10.3969/j.issn.1674-8115.2019.10.007
    Abstract425)      PDF(pc) (8837KB)(142)       Save
    Objective &middot; To construct scell lines expressing the large conductance Ca2+-activated K+ channel (MaxiK or BK) &alpha;-subunit and to explore the mechanism of potassium excretion via BK&alpha; channel. Methods &middot; The BK&alpha; plasmid with Myc tag was constructed and transfected into HEK293 cell lineslipofectamine 2000. The positive monoclonal cell lines were screenedG418, and the of BK&alpha; was detectedWestern blotting and the location of BK&alpha;immunofluorescence. The scell lines expressing BK&alpha; protein was cultured on slides to form a single cell layer, which was perfused with different potassium ion concentrations of 5 mmol/L and 100 mmol/L, and the single channel patch clamp recorded the ion flux of BK&alpha;. Wild type and mutants (G77R, G130R, C140R and R297C) of the inwardly rectifying potassium channel (Kir4.1) were transfected into HEK293 cells stably transfected with BK&alpha;, and then the membrane protein was extracted. The of BK&alpha; was detectedWestern blotting. Results &middot; Scell lines expressing BK&alpha; channel were selected HEK293 cells after transfection and cellular immunofluorescence verified the of BK&alpha; channel and its on the cell membrane. The channel open frequency (NPo) of BK&alpha; increased rapidly when perfused with 100 mmol/L potassium. After being transfected with wild type or mutants of Kir4.1, the membrane of BK&alpha; in the scell lines showed significant difference among these groups (PConclusion &middot; The HEK293 cell lines stably expressing BK&alpha; have been successfully constructed. BK&alpha; channel can be activatedhigh potassium solutions. The function of the BK&alpha; subunit can be related to Kir4.1 channel, which may be attributed to the depolarization of the cells transfectedKir4.1 mutants.
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    Evaluation of SDF-1 complex with CGF applied to canine dental-pulp-like tissue regeneration
    QIAO Lu1, WANG Hui-hui1, QI Ying2, FU Meng-chen1, YAN Yan-hong1, ZHAO Yu-mei1
       2019, 39 (10): 1148-.   DOI: 10.3969/j.issn.1674-8115.2019.10.008
    Abstract384)      PDF(pc) (10172KB)(159)       Save
    Objective &middot; To evaluate the feasibility of immature permanent teeth pulp regeneration with a new method that utilizes the integration of concentrated growth factor (CGF) as a scaffold and stromal cell-derived factor-1 (SDF-1). Methods &middot; Canine dental pulp cells (DPC) were isolated and cultured in vitro. Then the effects of SDF-1 and CGF were observed on DPC proliferation and differentiation. The pulpless model was established on the beagle&rsquo;s immature incisors which were divided into four groups: natural pulp (A), empty cannel (B), CGF-filling (C) and SDF-1/CGF-filling (D). After 10 weeks, specimens were checkedimaging examination, RT-PCR and histological observation. Results &middot; CGF extraction (CGFe) induced DPC proliferation while the combination of SDF-1 and CGFe enhanced this effect and also facilitated odontogenic and angiogenic differentiation of DPC. According to imaging examination, the apex growth of all four groups was in varying degrees. RT-PCR indicated the s of odontogenesis and angiogenesis related genes in group D were higher than those in group C. Besides, neonatal dentin and dental-pulp-like tissue were observed inside the canal of both group C and D, while only cementum-like tissue existed around root apex of group B. Conclusion &middot; SDF-1 plays an important role in driving the process of pulp-like-tissue regeneration of immature permanent teethusing CGF as an effective scaffold.
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    Improvement of liver fibrosis diagnostic models based on Youden index
    SANG Chao1, XIE Guo-xiang2, LIANG Dan-dan1, ZHAO Ai-hua1, JIA Wei1, 2, CHEN Tian-lu1
       2019, 39 (10): 1156-.   DOI: 10.3969/j.issn.1674-8115.2019.10.009
    Abstract431)      PDF(pc) (7663KB)(199)       Save
    Objective &middot;using Youden index, to improve the performance of the hepatic fibrosis diagnostic models, and to solve the problem of unbalanced diagnostic sensitivity when there is a big difference in the sample size of two groups. Methods &middot; Two hepatitis B virus (HBV) datasets available on GitHub were selected, including 482 HBV infected subjects recruited Shuguang Hospital in affiliation with Shanghai University of Traditional Chinese Medicine (train set) and 86 HBV infected subjects Xiamen Hospital of Traditional Chinese Medicine (validation set).using the two datasets, linear discriminant analysis model, random forest model, gradient boosting model and decision tree model were established, based on four clinical parameters (age, glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, and platelet count) of patients, for the diagnosis of early and advanced hepatic fibrosis as well as the diagnosis of hepatic fibrosis and cirrhosis. Youden index was used to adjust the threshold value and the classification result of each diagnostic model. The diagnostic performances of each machine learning model and fibrosis index based on the 4 factor (FIB-4) were evaluatedaccuracy, the area under the receiver operating characteristic curve (AUC) and sensitivity. Results &middot; The intergroup sensitivity imbalance occurred in all machine learning models. After using Youden index, the difference of intergroup sensitivity was greatly reduced, and the total accuracy and AUC values of machine learning models were generally higher than those of FIB-4 index. Conclusion &middot; The improved diagnostic models based on Youden index can reduce the difference of intergroup sensitivity and improve the comprehensive performance of the diagnostic models of hepatic fibrosis.
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    Distribution characteristics of fragmented QRS in patients with coronary atherosclerotic heart disease and its relationship with left ventricular remodeling
    SU Hai-xia, ZHU Ya-qin, ZHANG Tian-kuang, ZHANG Hui-li, GU Jun
       2019, 39 (10): 1162-.   DOI: 10.3969/j.issn.1674-8115.2019.10.010
    Abstract335)      PDF(pc) (6913KB)(131)       Save
    Objective &middot; To explore the diagnostic value of fragmented QRS (fQRS) for coronary atherosclerotic heart disease (CHD), and to analyze its relationship with left ventricular remodeling. Methods &middot; Nov. 2016 to Oct. 2018, 498 hospitalized patients in the Department of Cardiovascular Medicine of Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine were selected consecutively. During the hospitalization, all the patients underwent coronary angiography. According to the angiographic results, the patients were divided into the control group (203 patients with negative or coronary stenosis &ge;75%). The incidences of fQRS(+) in the normal electrocardiogram among the three groups were comparedchi-square test of R&times;C contingency table. Two hundred and thirty patients with single-vessel stenosis&ge;30% were divided into the anterior descending branch group (128 cases), the right coronary branch group (59 cases), and the circumflex branch group (43 cases), and the relationship between fQRS(+) leads and diseased vessels was analyzednonparametric test. Finally, all the patients were divided into fQRS(+) group (86 cases) and fQRS(-) group (412 cases). The correlation between fQRS and left ventricular ejection fraction (LVEF), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), interventricular septum thickness (IVST) and left ventricular posterior wall thickness (LVPWT), respectively, were analyzedbinary Logistic regression model. Results &middot; The chi-square test of R&times;C contingency showed that the incidences of fQRS(+) in the three groups were 8.89%, 16.13% and 30.71%, respectively, with statistically significant differences (all P Ⅱ, Ⅲ, AVF, V1, V2) were more common in the right coronary branch group, the fQRS(+) leads reflecting upper lateral wall (Ⅰ, AVL) were more common in the circumflex branch group, with statistically significant differences (all P0.05). Binary Logistic regression analysis showed that fQRS was negatively correlated with LVEF (r-0.030, OR0.971, 95% CI 0.945-0.997, P0.029), and positively correlated with LVESV (r0.042, OR1.043, 95% CI 1.005-1.082, P0.026). Conclusion &middot; fQRS has certain reference value in the clinical diagnosis of CHD, and left ventricular remodeling may be one of the mechanisms of fQRS.
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    Association of serum olanzapine concentration and cytochrome P450 1A2 gene polymorphisms: a meta-analysis
    WANG Ye-wei, CHEN Yan, FANG Xin-yu, ZHANG Yi, CAI Jun, SONG Li-sheng, ZHANG Chen
       2019, 39 (10): 1167-.   DOI: 10.3969/j.issn.1674-8115.2019.10.011
    Abstract396)      PDF(pc) (6944KB)(160)       Save
    Objective · To investigate association of cytochrome P450 1A2 (CYP1A2) gene polymorphisms and serum olanzapine concentration. Methods · Key words including “cytochrome P450 CYP1A2”, “genetic polymorphism”, “antipsychotic”, “olanzapine”, “schizophrenia”, and “serum concentration” were used to search in databases, including SinoMed, Wanfang, Weipu, CNKI, PubMed, Embase and Cochrane Library. The search period was limited the establishment of the database to February 27th, 2018. STATA/SE 12.0 package was applied to conduct statistical analyses. Results · Four studies with a total of 330 schizophrenic patients were included. In the recessive model of CYP1A2 gene -163C>A SNP, patients with A/A genotype had significantly lower olanzapine concentration/dosage ratio (C/D) than patients with C/C or C/A genotype. Conclusion · A/A homozygote of CYP1A2 gene -163C>A SNP is significantly associated with the decreased olanzapine serum concentration.
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    Association study of SNAP25 gene polymorphisms with schizophrenia in Chinese Han population
    WU Yi1, WU Yi-ming1, SONG Bing-fu1, HUANG Xin-xin2, Lü Qin-yu2, YI Zheng-hui2
       2019, 39 (10): 1172-.   DOI: 10.3969/j.issn.1674-8115.2019.10.012
    Abstract416)      PDF(pc) (7414KB)(175)       Save
    Objective · To investigate the relationship between synaptosomal-associated protein of 25 000 (SNAP25) gene and schizophrenia (SZ) in Chinese Han population. Methods · TaqMan probe genotyping technique was used to detect 5 single nucleotide polymorphisms (SNPs) of SNAP25 gene in 434 SZ patients (case group) and 432 healthy controls (control group). Rs3746544, rs8636, rs362998, rs6039769 and rs6077690 were genotyped to compare the alleles, genotypes, genotypes under different genetic models and haplotype frequency distributions between the case group and the control group. Results · The difference of frequency distribution of rs362998 and rs6039769 alleles was statistically significant between the case group and the control group (P0.029, P0.033, respectively). After Bonferroni correction, the differences were not statistically significant (all P>0.05). There was a statistically significant difference in the distribution of rs362998 and rs3746544 genotypes between the case group and the control group (P0.005, P0.043, respectively). There was still a statistically significant difference in rs362998 after correction (P0.025), but there was no statistical difference in rs3746544 (P>0.05). The rs362998 locus was statistically significant between the co-dominant and dominant-genetic models (P0.003), and the difference was still statistically significant after Bonferroni correction (P0.015). Under the codominant and recessive inheritance patterns, the difference of rs3746544 genotype distribution was statistically significant between the groups (P0.042, P0.012, respectively), and the difference was not statistically significant after correction (P>0.05). The differences of frequency distribution of all haplotypes consisting of rs3746544-rs8636 between the case group and the control group were not statistically significant (P>0.05). Conclusion · In Chinese Han population, SNAP25 may be a susceptibility gene for schizophrenia, and rs362998 may be involved in the pathogenesis of schizophrenia.
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    Expression and clinical significance of long-chain non-coding RNA lincRNA-BBOX1-2 in gastric cancer
    SUN Ying1, HU Mei-jie2, GU Wei1, LI Jian1, WANG Ji1, ZHENG Xiong1
       2019, 39 (10): 1178-.   DOI: 10.3969/j.issn.1674-8115.2019.10.013
    Abstract393)      PDF(pc) (5933KB)(199)       Save
    Objective &middot; To evaluate the of long-chain non-coding RNA (lncRNA) lincRNA-BBOX1-2 in gastric cancer tissues and the value of diagnosis and prognosis of gastric cancer. Methods &middot; The of lincRNA-BBOX1-2 was detectedreal-time quantitative PCR in 45 cases of gastric cancer and adjacent normal tissues. The correlations of lincRNA-BBOX1-2 with clinic-pathological features and clinical prognosis were analyzed. Results &middot; The higher of lincRNA-BBOX1-2 was observed in the gastric cancer tissues (3.291&plusmn;0.274 vs 1.125&plusmn;0.075, P0.000) as compared with the adjacent normal tissues. Up-regulation of lincRNA-BBOX1-2 was associated with positive lymph node metastasis (P0.005, r0.172) and advanced clinical stage (P0.013, r0.137). Receiver operating characteristic curve analysis showed that the area under the curve (AUC) value was 0.916 (95%CI 0.859&ndash;0.972) for lincRNA-BBOX1-2 in predicting the occurrence of gastric cancer, and 0.720 (95%CI 0.565&ndash;0.875) for lincRNA-BBOX1-2 in predicting the lymph node metastasis. Conclusion &middot; The up-regulation of lincRNA-BBOX1-2 in gastric cancer tissues is associated with the clinic-pathological features of gastric cancer. lincRNA-BBOX1-2 may be used as a potential diagnostic biomarker in patients with gastric cancer.
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    Incidence and risk factors of infection related to totally implanaccess port in upper arm in cancer patients
    QIU Xiao-xia1*, JIN Guang-xin2*, GUO Yan1,DAI Meng-jun2, HU Jia-xian1, ZHANG Xue-bin2
       2019, 39 (10): 1183-.   DOI: 10.3969/j.issn.1674-8115.2019.10.014
    Abstract415)      PDF(pc) (7707KB)(194)       Save
    Objective &middot; To explore the incidence and risk factors of infection related to totally implanaccess port (TIAP) in upper arm in cancer patients. Methods &middot; A total of 1 961 patients who received TIAP for deliverance of chemotherapy at Renji Hospital, Shanghai Jiao Tong University School of Medicine during the period February 2014 to December 2018. The general clinical data, catheterization status, bacteriological data and incidence of TIAP-related infections were retrospectively analyzed. The univariate analysis and multivariate Logistic regression analysis were used to determine the risk factors TIAP-related infections. Results &middot; A total of 43 patients developed TIAP-related infections, with an incidence of 2.2% (43/1 961). The mean time interval the implantation of upper arm TIAP to the diagnosis of infection was (116.88&plusmn;95.39) d (7-377 d). In univariate analysis, gender (P0.001), catheter diameter (P0.000), upper extremity venous thrombosis (UEVT) (P0.000) and outpatient (P0.005) were significantly associated with the risk of infection related to upper arm TIAP. Multivariate binary Logistic regression analysis showed that gender (OR0.35, P0.012), UEVT (OR31.59, P0.000) and catheter diameter (OR2.81, P0.005) were three significant independent predictors of infection related to upper arm TIAP. Conclusion &middot; Gender, UEVT and catheter diameter are independent risk factors for upper arm TIAP-related infection.
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