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    Journal of Shanghai Jiao Tong University (Medical Science) 2020 Vol.40
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       2020, 40 (1): 1-.  
    Abstract365)      PDF(pc) (2681KB)(347)       Save
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    Electron microscopic study of the human THOC1-THOC2-THOC3 subcomplex
    LIU Yong-mei, TAN Ming, LEI Ming
       2020, 40 (1): 1-.   DOI: 10.3969/j.issn.1674-8115.2020.01.001
    Abstract631)      PDF(pc) (9981KB)(614)       Save
    Objective · To study the structure of the human THOC1-THOC2-THOC3 subcomplexnegative-staining electron microscopy. Methods · The recombinant protein components were co-expressed in cultured insect cells, and the protein complex was isolated via sequential Ni-NTA and Strep-Tactin affinity purification followedSuperose gel filtration chromatography. The purified protein sample was then subjected to negative-staining electron microscopy and single particle image analysis. Results · The recombinant human THOC1-THOC2-THOC3 complex with high purity and good homogeneity was obtainedusing a tandem affinity purification scheme with Ni-NTA and streptavidin-based chromatography. Preliminary study on the structure of human THOC1-THOC2-THOC3 subcomplex was achievedelectron microscopy using negative staining with uranium formate. Conclusion · A low resolution model of human THOC1-THOC2-THOC3 subcomplex was achievedsingle particle reconstruction.
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       2020, 40 (1): 2-.  
    Abstract400)      PDF(pc) (4594KB)(353)       Save
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       2020, 40 (1): 3-.  
    Abstract453)      PDF(pc) (1315KB)(252)       Save
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    Changes of ATP metabolism and purinergic receptors in inflammatory response stage of diabetic wound healing
    WANG Qi, ZHU Guan-ya, XIE Ting, GE Kui, NIU Yi-wen
       2020, 40 (1): 10-.   DOI: 10.3969/j.issn.1674-8115.2020.01.002
    Abstract925)      PDF(pc) (9975KB)(452)       Save
    Objective · To investigate the changes of extracellular adenosine triphosphate (ATP) metabolism and its related indices of inflammatory response stage during diabetic wound healing. Methods · Ninety-six C57BL/6J mice were divided into 4 groups randomly, i.e. normal control group (NC, n24), normal with ATP applied group (NA, n24), diabetes control groups (DC, n24), and diabetes with ATP applied group (DA, n24). The mice in DA and DC group were inducedstreptozocin multiple intraperitoneal injection. Full-thickness excisional wound was created on the dorsum of all mice. Distilled water was applied in NC and DC group. ATP was applied in NA and DA group. Hematoxylin and eosin staining (HE staining) was used to observe neutrophils infiltration. Myeloperoxidase deficiency (MPO) contents were detectedenzyme-linked immunosorbent assay (ELISA). The contents of ATP and its metabolites in tissues of wound margin were detectedultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Immunohistochemistry was utilized to investigate the of Connexin43, Pannexin1, CD39, P2X7 and P2Y2. Healing speed were assessedthe calculation of healing rate on each point. Results · ①During the inflammatory response stage of wound healing, the MPO contents, neutrophils infiltration numbers, the ATP contents, and the s of Connexin43, Pannexin1, CD39, P2X7 and P2Y2 of mice in DC group were all lower than those in NC group (all PP0.000). Conclusion · Inflammatory response is insufficient on the early stage of diabetic wound healing, while ATP, ATP hydrolase and purinergic receptor also remain at low level at the same time. To a certain extent, exogenous application of ATP can accelerate diabetic wound healing rate.
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    Association of SIRT1 gene with major depression and its clinical features
    ZHAO Jun-xiong, HUA Shen, FAN Wei-xing, WANG Wei-ping, TANG Wei, ZHANG Chen
       2020, 40 (1): 18-.   DOI: 10.3969/j.issn.1674-8115.2020.01.003
    Abstract1006)      PDF(pc) (5066KB)(544)       Save
    Objective · To explore the relationship between rs3758391 polymorphism of SIRT1 gene and depressive symptoms, and to further understand the role of SIRT1 gene in major depressive disorder. Methods · A total of 323 patients with major depressive disorder were retrospectively collected the Jinhua Second Hospital, Wenzhou Kangning Hospital and Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine. A total of 347 healthy subjects were also recruited. Depressive symptoms were evaluatedusing the Hamilton Depression Scale (HAMD), and rs3758391 polymorphism was genotypedusing the TaqMan SNP genotyping Assay. The effect of rs3758391 polymorphism on the of SIRT1 mRNA in brain was analyzedBRAINEAS database, and the difference of depressive symptom severity among three genotypes at rs3758391 polymorphism was comparedmultivariate analysis of variance. Results · The frequencies of C and T alleles of rs3758391 polymorphism in SIRT1 gene were 18.7% and 81.3% in the case group, and 14.3% and 85.7% in the control group, respectively. The allelic distribution frequencies between the two groups were significantly different (χ24.86, P0.03). There were significant differences in mood, cognitive impairment and HAMD scores among patients with different genotypes of rs3758391 polymorphism (PSIRT1 gene in occipital cortex (OCTX) (P0.003). Conclusion · rs3758391 polymorphism of SIRT1 gene may be a risk factor for major depressive disorder in Chinese Han population, and is associated with the severity of depressive symptoms, especially with emotional symptoms and cognitive impairment.
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    Fatty acid quantification methodisobutyl esterification and GC-FID/MS
    GAN Miao-yan, XIE Hui, TANG Hui-ru
       2020, 40 (1): 22-.   DOI: 10.3969/j.issn.1674-8115.2020.01.004
    Abstract734)      PDF(pc) (9544KB)(405)       Save
    Objective · To establish a quantification method for understanding the varieties and concentrational changes of fatty acids under physiological and pathophysiological conditions. Methods · Based on isobutyl esterification using (-)-menthyl chloroformate and isobutanol, 27 typical fatty acids were qualitatively and quantitatively analyzedgas chromatography-flame ionization detector/mass spectrometry (GC-FID/MS). The sensitivity and stability of the method were detectedlimit of detection (LOD), limit of quantification (LOQ), and intra-day and inter-day relative standard deviation (RSD). The method was applied to four typical biological samples (human serum, urine, feces and rat liver) to verify the universality in different substrates. Results · Isobutyl esters of 27 fatty acids were effectively separated with an HP-5MS column. The results showed nice linearity within 2-3 orders of magnitude in terms of concentration with R2>0.99 for all 27 tested fatty acids. The LODs of the method were between 0.03 and 2.96 pmol on column whereas the LOQs were between 0.09 and 9.86 pmol. The results of method validation showed that the intra-day and inter-day RSDs were all under 10% in the range of high, medium and low concentrations. With this method, moreover, 10, 7, 14 and 9 fatty acids were detected in human serum, urine, feces and rat liver, respectively. Conclusion · A quantitative analytical method for fatty acids with short, media and long chains (C1-C24) is established based on isobutyl estrification in aqueous media. This method has the advantages of mild condition, high sensitivity, good stability, and simple and quick operation, and can be directly used in the detection of serum, urine, feces, liver and other biological samples, which may provide a new idea for high-throughput metabolome analysis.
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    Effect of Lycium barbarum polysaccharide and aerobic exercise on rats with non-alcoholic fatty liver disease and its mechanism
    GUO Yi-qiong, WU Qiong, WU Ya-ting, GAO Lu-lu, YANG Jian-jun
       2020, 40 (1): 30-.   DOI: 10.3969/j.issn.1674-8115.2020.01.005
    Abstract589)      PDF(pc) (9012KB)(281)       Save
    Objective · To investigate the effects of different doses of Lycium barbarum polysaccharides (LBP) and aerobic exercise on rats with non-alcoholic fatty liver disease (NAFLD) and its mechanisms. Methods · SD rats of 8 weeks old were randomly divided into normal control group and high fat diet (HFD) group. The normal control group was given normal diet, and the HFD group was given HFD for 10 weeks to construct a NAFLD rat model. After successful modeling, the rats with NAFLD were assigned into four groups with eight in each, namely: HFD group, LBP-a group (50 mg/kg), LBP-b group (100 mg/kg) and aerobic exercise group (20 m/min, 60 min/d). After the intervention for 6 weeks, fasting for 12 hour and fasting blood glucose level was measured. Blood samples and liver tissues of rats were collected after intraperitoneal anesthesia with 10% chloral hydrate. The levels of glutamate pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein (LDL), high density lipoprotein (HDL), malondialdehyde (MDA), superoxide dismutase (SOD) and irisin in rats were measured according to corresponding protocols of kits. The s of peroxisome proliferators-activated receptor-γ coactivator-1 α (Pgc1-α) and fibronectin type Ⅲ domain-containing protein 5 (Fndc5) in liver were detectedusing reverse transcription polymerase chain reaction. Results · Compared with the HFD group, the levels of GPT, GOT, TC, TAG, MDA and homeostasis model assessment of insulin resistance (HOMA-IR) were significantly decreased in LBP-a group, LBP-b group and aerobic exercise group (PPPPgc1-α mRNA in LBP-b group and aerobic exercise group were up-regulated as compared with the HFD group (PFndc5 mRNA in three intervention groups were up-regulated as compared with the HFD group (PConclusion · LBP and aerobic exercise can significantly improve lipid metabolism disorder and decrease liver oxidative stress in rats with NAFLD, which may be related to the up-regulated s of Pgc1-α mRNA and Fndc5 mRNA.
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    Establishment of a lung cancer momodel with humanized immunity and its role in efficacy evaluation of programmed death-1 inhibitors
    ZHOU Zhen, XU Yun-hua, ZHANG Fei-fei, ZHAO Yi, GUO Lin, FU Guo-long, SHEN Sheng-ping, LENG Xue-jiao
       2020, 40 (1): 37-.   DOI: 10.3969/j.issn.1674-8115.2020.01.006
    Abstract922)      PDF(pc) (8899KB)(449)       Save
    Objective · To establish a lung cancer momodel with humanized peripheral blood mononuclear cells (PBMC) expressing programmed death-ligand 1 (PD-L1), and study the role of the model in evaluating the efficacy of programmed death-1 (PD-1) inhibitors. Methods · Fresh biopsy tissue samples or tumor cells in malignant pleural effusion the patients with advanced non-small cell lung cancer were inoculated subcutaneously in CB17-SCID mice to establish patient-derived xenograft (PDX) models. The of PD-L1 in PDX models was detectedimmunohistochemistry. The mature human PBMC and PDX model tumor cells were mixed and then inoculated into NCG mice to establish a PDX model of lung cancer with humanized immunity, on which the efficacy of PD-1 inhibitor was verified. Results · Among the PDX models established16 clinical samples, 2 were strongly positive for PD-L1, 4 were positive, and the rest were negative. In the PDX model with strongly positive PD-L1, the tumor growth inhibition rate of cindilimab, an inhibitor of PD-1, was 82.6%, 21 days after the initial administration; in the PDX model with negative PD-L1, the inhibitor of PD-1 showed no antitumor activity. Conclusion · A PD-L1-expressing lung cancer momodel with humanized immunity is successfully established and the efficacy of PD-1 inhibitor can be evaluated on the model.
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    Effects of low-carbohydrate diet on body weight and glycolipid metabolism in normal rats and obese rats
    PENG Rong, YANG Li-jun, DENG Mao-lin
       2020, 40 (1): 44-.   DOI: 10.3969/j.issn.1674-8115.2020.01.007
    Abstract831)      PDF(pc) (9916KB)(407)       Save
    Objective · To explore the effects of low-carbohydrate diet (LCD) on body weight and glycolipid metabolism in obese rats and normal weight rats. Methods · Eighty male normal weight Sprague-Dawley rats were divided randomly into three groups, i.e., normal control diet group (CD group, n10), LCD group (n10) and traditional high-fat diet group (HFD group, n60). After eight weeks, 30 obese rats were selected HFD group. Then the obese rats were divided randomly into three groups. The feed of two groups was changed HFD to LCD (HFD-LCD group) or CD (HFD-CD group), and the rest group was fed with HFD continuously. The experiment lasted for another eight weeks. The feed of CD group and LCD group remained unchanged. The measurement indicators included body weight, feed intake, visceral fat, and blood biochemical indexes (fasting blood glucose, triacylglycerol, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and serum β-hydroxybutyrate concentration). Results · In normal rats, the 6th week, the body weight of LCD group was significantly lower than that of HFD group (PP>0.05). But at the end of the 16th week, total cholesterol of LCD group was significantly higher than that of CD group (P0.001) and high-density lipoprotein cholesterol was lower (P0.021). In the obese rats, at the end of the 16th week, the body weight of HFD-LCD group was significantly lower than that of HFD group and HFD-CD group (PPP>0.05). In the obese rats, compared with HFD group, the intervention of HFD-LCD (β-88.56, P0.000) and HFD-CD (β-39.08, P0.007) resulted in the decrease of body weight. β-hydroxybutyrate level was helpful to weight loss in the range of 1-2 times of HFD-CD group (β-34.92, P0.006). Conclusion · LCD may have different effects on body weight and lipid metabolism between normal weight rats and obese rats, LCD has no weight loss effect on the normal weight rats, but can increase total cholesterol; however, in obese rats LCD can reduce weight and improve the metabolism of glycolipid.
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    Sleep apnea hypopnea syndrome prolongs rapid eyes movement sleep time in depressive state and shortens deep sleep time in manic state in patients with bipolar disorder
    JIN Zhi-xing, WANG Mei-ti, ZHOU Qian, Lü Dong-bin, WANG Fan, HUANG Qin-te, WU Zheng-lin, LI Hai-bin, XU Chu-chen, CAO Lan, ZHAO Jie, CAO Tong-dan, HUANG Hai-jing, FANG Yi-ru, HONG Wu
       2020, 40 (1): 51-.   DOI: 10.3969/j.issn.1674-8115.2020.01.008
    Abstract843)            Save
    Objective · To observe the effect of sleep apnea hypopnea syndrome (SAS) on sleep staging of bipolar disorder patients in different states. Methods · A total of 210 healthy controls aged 18–65 and 235 bipolar disorder patients of the same age were collected. The bipolar disorder patients were divided into depressive, manic and mixed states. Sleep time structure, sleep posture, heart rate and other indicators were collected by using sleep quality assessment system based on cardiopulmonary coupling analysis. According to whether the apnea-hypopnea index (AHI) was ≥ 5 times/h, whether they had SAS were determined. Two-way multivariate analysis of variance was conducted, investigating the effect of clinical states and SAS, on several sleep indicators. Results · SAS significantly prolonged rapid eyes movement (REM) sleep time of patients in depressive state (P=0.000) and shortened deep sleep time of patients in manic state (P=0.011). In addition, the heart rate during sleep (including deep sleep, light sleep and REM sleep) of patients in manic state increased most significantly among the three clinical states (P=0.000). Lying supine aggravated SAS most significantly in manic state among the three clinical states (P=0.002). Conclusion · SAS has different effects on the sleep staging of bipolar disorder patients in different states.
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    Pregnancy outcome comparison between primiparas and multiparas with advanced maternal age
    LIN Jing, LIU Han, LIU Xin-mei, CHEN Xiao-jun, HUANG He-feng
       2020, 40 (1): 58-.   DOI: 10.3969/j.issn.1674-8115.2020.01.009
    Abstract1013)      PDF(pc) (7053KB)(454)       Save
    Objective · To explore the risk of adverse pregnancy outcomes in the primiparas and the multiparas with advanced maternal age. Methods · Through a retrospective cohort study, 6 129 elderly women who gave birth to a single child January 2014 to December 2016 in the International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine were collected to compare the differences in pregnancy outcomes between the primiparas and the multiparas (parity2). Logistic regression analysis was used to evaluate the risk of adverse pregnancy outcomes in the two groups of pregnant women. Results · Compared with the multiparas, the primiparas with advanced maternal age had a higher risk of premature rupture of membranes (adjusted OR1.35, 95% CI 1.18-1.54), preeclampsia/eclampsia (adjusted OR1.99, 95% CI 1.45-2.73), gestational diabetes mellitus (adjusted OR1.32, 95% CI 1.15-1.51), small-for-gestation age (adjusted OR2.49, 95% CI 1.78-3.48), and cesarean section (adjusted OR1.45, 95% CI 1.30-1.62). The birth weight of infants deliveredthe primiparas was slightly lower than that of the multiparas. Conclusion · In the advanced maternal age women, primiparas have a higher risk of adverse perinatal outcomes than multiparas, such as premature rupture of membranes, preeclampsia/eclampsia, gestational diabetes mellitus, small-for-gestation age and cesarean section.
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    Imaging law of postoperative electrode locations in deep brain stimulation for Parkinsons disease
    WANG Tao, ZHANG Chen-cheng, LI Dian-you, SUN Bo-min, FU Meng
       2020, 40 (1): 64-.   DOI: 10.3969/j.issn.1674-8115.2020.01.010
    Abstract784)      PDF(pc) (8236KB)(360)       Save
    Objective · To explore the potential regularity and caof the differences between magnetic resonance imaging (MRI) and computed tomography (CT) presenting electrodes after deep brain stimulation (DBS) for Parkinsons disease (PD), into provide reference for optimizing clinical decision. Methods · Forty-nine PD patients who underwent DBS treatment in Department of Functional Neurosurgery of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Jan. 2016 to Dec. 2017 were selected. CT images were acquired 3-5 days after surgery and MRI images were acquired during 3-6 monthsthe follow-up of regular programming. The fused images of short-term CT combined with preoperative MRI and long-term MRI respectively were compared to show the differencethe tip contact positions of electrodes. Results · A total of 50 groups of postoperative CT fused images and MRI images were obtained. The space differences of the two types of images were 1.36 (0.98, 1.70) mm (P0.021) and 1.28 (0.99, 1.88) mm (P0.006), on the right and left electrodes, respectively. Bilateral electrodes in both short-term and long-term images had a tendency to move to the medial, rear, and bottom part of the brain. Conclusion · The potential tendency in shifts of DBS electrodes can provide reference for establishing the brain drift model and optimizing the position of the implanted electrode.
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    ARHGEF16 variants screening and mutation function analysis for children with total anomalous pulmonary venous connection
    WANG Jing, JIN Li-hui, ZHANG Qi, SUN Kun, YU Yu
       2020, 40 (1): 70-.   DOI: 10.3969/j.issn.1674-8115.2020.01.011
    Abstract699)      PDF(pc) (7314KB)(311)       Save
    Objective · To investigate the pathogenesis of total anomalous pulmonary venous connection (TAPVC), and to identify ARHGEF16 gene through full exon sequencing screening and analyze its mutation function. Methods · The blood, clinical data and auxiliary examination results of 78 children with TAPVC and 100 healthy controls were collected, and the genomic DNA was extracted for ARHGEF16 mutation screening. ARHGEF16 wild-type and mutant plasmids were constructed and transfected into 293T cells. mRNA and protein levels were detectedquantitative real-time PCR (RT-qPCR) and Western blotting, respectively. Protein-protein interaction exploration was performedCytoscape software. Results · Two novel variants c.C236>T (A79V) and c.G619>C (G207R) were found in TAPVC patients and were not found in healthy controls. Compared with the wild type, the mutants ARHGEF16 were up-regulated in both mRNA and protein levels. Protein interaction analysis showed that ARHGEF16 and RAC1 were directly associated; RAC1 was up-regulated in HEK293 cells with ARHGEF16 over through RT-qPCR. Conclusion · The missense mutations of ARHGEF16 affect the mRNA and protein levels of ARHGEF16. Over of ARHGEF16 up-regulates the of RAC1, suggesting that it may participate in the development and formation of TAPVCregulating RAC1.
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    Diagnostic efficiency of ultrasound TI-RADS combined with three-dimensional shear wave elastography in thyroid microcarcinoma
    HAN Rui-jun, DU Jing, CHEN Cui, ZHU Cai-xia, LI Feng-hua, WANG Jia-dong
       2020, 40 (1): 76-.   DOI: 10.3969/j.issn.1674-8115.2020.01.012
    Abstract659)      PDF(pc) (7496KB)(289)       Save
    Objective · To evaluate the diagnostic value of ultrasound thyroid imaging-reporting and data system (TI-RADS) combined with three-dimensional shear wave elastography (3D-SWE) technique in thyroid microcarcinoma. Methods · Jun. 2017 to Jan. 2018, 66 patients with 67 thyroid nodules in the Department of Ultrasound of Renji Hospital, Shanghai Jiao Tong University School of Medicine were recruited. All thyroid nodules were classified as TI-RADS 4-5, and the maximum diameter was 5.0-10.0 mm. After routine ultrasound examination, all thyroid nodules were evaluatedTI-RADS classification, 3D-SWE technique, fine-needle aspiration biopsy (FNAB) examination and an additional BRAFV600E gene mutation test. All the nodules were divided into benign group and malignant group according to the results of surgical pathology or fine-needle aspiration combined with BRAFV600E gene detection. The threshold value of the parameters in 3D-SWE for differentiating benign and malignant thyroid nodules was calculated, and the TI-RADS was further adjustedYoungs modulus parameters of the lesion to obtain combined TI-RADS (conventional ultrasound combined with 3D-SWE) for detecting the benign and malignant nodules. Results · Among the 67 thyroid nodules, 38 of them were malignant and 29 were benign. The optimal cut-off point of three-dimensional maximum Youngs modulus in sagittal plane (3D-S-Emax) was 24.6 kPa. The area under the curve (AUC) value, sensitivity, specificity and accuracy of 3D-S-Emax were 0.683, 65.8%, 65.5% and 65.7%, respectively. The AUC value for combined TI-RADS was 0.801 and that for conventional TI-RADS was 0.794, without statistical difference. The sensitivity, specificity and accuracy of conventional TI-RADS and combined TI-RADS were 63.2%, 82.8%, 71.6%, and 86.8%, 69.0%, 79.1%, respectively. Only sensitivity of combined TI-RADS was significantly higher than that of conventional TI-RADS (P0.004). Conclusion · Combined TI-RADS and conventional TI-RADS have similar diagnostic value in thyroid microcarcinoma, while combined TI-RADS has higher diagnostic sensitivity and lower missed diagnosis rate.
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    Meta-analysis of effect and safety of repetitive transcranial magnetic stimulation on patients with schizophrenia
    HUANG Yan, ZHANG Xian-gao, LIN Heng, CHENG Shu-ying
       2020, 40 (1): 81-.   DOI: 10.3969/j.issn.1674-8115.2020.01.013
    Abstract763)      PDF(pc) (7804KB)(274)       Save
    Objective · To evaluate the efficacy and safety of repetitive transcranial magnetic stimulation (rTMS) for schizophreniameta-analysis, and provid evidence support for clinical treatment of schizophrenia. Methods · Randomized controlled trials of rTMS and schizophrenia were searched on Embase, PubMed, Cochrane Library, CNKI, Wanfang and VIP. The quality of the included literature was controlled and meta-analysis was performed with Stata 15.1 software. Results · Seventeen studies were enrolled, including 486 patients in the stimulation group and 436 in the control group. rTMS was effective in treating patients with schizophrenia (OR3.32, 95% CI 1.93—5.70, Z4.35, P0.000); rTMS treatment was effective for overall condition and negative symptoms of patients with schizophrenia (SMD-0.33, 95% CI -0.58—-0.08, Z2.61, P0.009; SMD-0.41, 95% CI -0.65—-0.17, Z3.31, P0.001), and ineffective for positive symptoms (P0.755). There was no significant difference in adverse reactions between the two groups (P0.732). Subgroup analysis revealed that only high frequency (10 Hz) stimulation and treatment for 4 weeks could achieve therapeutic effects. Conclusion · rTMS is effective in treating negative symptoms of patients with schizophrenia and has high therapeutic safety, but it is ineffective for positive symptoms.
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    Evaluation of glide path preparation and shaping ability of two nickel-titanium engine driven systems in moderate curved root canals
    TAI Zhao-wei, PENG Wei-wei, DU Rong, ZHU Ya-qin
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (1): 89-.   DOI: 10.3969/j.issn.1674-8115.2020.01.014
    Abstract789)      PDF(pc) (6292KB)(480)       Save
    Objective · To evaluate the clinical application effect of two nickel-titanium engine driven systems on moderate curved root canals. Methods · Ninety mesial root canals with moderate curvature of extracted human first mandibular permanent molar were randomly divided into three groups: M3 PRO Group, MTwo group and K group. The glide path was firstly preparedusing 10/04 file in M3 PRO group and MTwo group and 10 file in K group respectively .Then the preparation of root canals up to 25 was performed according to the instruction manual in each different group using standardized technique. All data including time required in glide path preparation, shaping preparation, total root canals preparation and changes of working length in root canal were recorded and analyzed statisticallyusing Analysis of Variance (ANOVA) and LSD-t tests (PResults · Root canal glide path preparation time: MTwo group showed significantly less preparation time while M3 PRO group exhibited significantly the longest preparation time. There was significant difference between MTwo group and M3 PRO group (P0.001). Root canal shaping preparation time: M3 PRO group showed significantly less preparation time while K group exhibited significantly the longest preparation time. Both MTwo group and M3 PRO group showed significantly less preparation time than K group (P0.004, P0.001), but there was no significant difference between MTwo group and M3 PRO group (P0.573). Total root canal preparation time: MTwo group showed the shortest time while K group exhibited the longest time. There were significant differences between MTwo group, M3 PRO group and K group (P0.004, P0.033), but no significant difference between MTwo group and M3 PRO group (P0.451). Changes of root canal working length: There were significant differences among the three groups (P0.037, P0.037, P0.000). M3 PRO group showed a minimal change while K group exhibited significant changes. Conclusion · MTwo nickel-titanium engine driven system with 10/04 file preform a high efficiency in glide path preparation, whilst M3 PRO nickel-titanium engine driven system show less changes in working length. Under the circumstancesthe total time required for root canal preparation is not significant, M3 PRO nickel-titanium engine driven system is more suifor root canal preparation in moderate curved root canals.
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    Analysis of suicidal ideation and its related factors among unmarried female miworkers in some factories in Shanghai and Guangzhou
    ZHOU Ni, SHEN Qiu-ming, SHI Yue, ZHANG Shu-xian, WANG Hu-wen, CHANG Rui-jie, CAI Yong
       2020, 40 (1): 93-.   DOI: 10.3969/j.issn.1674-8115.2020.01.015
    Abstract581)      PDF(pc) (10106KB)(282)       Save
    Objective · To investigate the suicidal ideation among unmarried female miworkers in Shanghai and Guangzhou and explore the association between suicidal ideation and some psychosocial problems, reproductive health factors. Methods · A questionnaire survey was conducted among 3 553 unmarried female miworkers in two factories in Shanghai and two in Guangzhouconvenient sampling. Logistic regression was used to analyze the related factors of suicide ideation including psychosocial problems and reproductive health factors. Results · Overall, 287(8.1%) of the respondents had suicidal ideation during the past year. Univariate Logistic regression showed that alcohol, low self-esteem, depression, anxiety, loneliness, poor social support (emotional support and economic support), favorable attitude toward premarital pregnancy, favorable attitude toward multiple induced abortion, experience of unplanned pregnancy and experience of induced abortion were related to suicidal ideation after demographic factors being corrected. Multivariate Logistic regression showed that alcohol (OR1.42, 95% CI 1.04–1.93), low self-esteem (OR1.47, 95% CI 1.03–2.11), loneliness (OR3.30, 95% CI 2.42–4.51), poor emotional support (OR2.68, 95% CI 2.00–3.61) and poor economic support (OR3.79, 95% CI 2.86–5.04) were the critical factors. Conclusion · The factors such as risk behavior, mental health, lack of social support were associated with increased odds for suicidal ideation among the unmarried female miworkers. The prevention and intervention mechanism should be constructed, and the social support should be enhanced to effectively prevent the occurrence of suicidal ideation.
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    Influence of emotional intelligence on behavior performance in crisis intervention situations
    DONG Min-ye, JIA Zhi-ying, SHI Zhen-su, LI Guo-hong
       2020, 40 (1): 101-.   DOI: 10.3969/j.issn.1674-8115.2020.01.016
    Abstract669)      PDF(pc) (7989KB)(381)       Save
    Objective · To explore the effect of emotional intelligence on the behavioral performance in crisis intervention, taking the knowledge and skills learning performance of crisis intervention as the mediating variable. Methods · Based on the sequential pattern analysis and comprehensive evaluation of learning performance in the previous study, Emotional Intelligence Scale was used to collect the emotional intelligence of 104 mental health service personnel. The mediating effect model was used to analyze the relationship among personal traits, learning performance and behavior performance. Results · There was no direct correlation between emotional intelligence and behavior performance (r-0.016, P0.869). The knowledge and skills learning performance of crisis intervention in identifying problems, providing support and coping strategy played a complete mediating role between emotional intelligence and behavior, with indirect effects of a1b1-0.052 (P0.010, 95% CI -0.126 - -0.010), a2b20.139 (P0.000, 95% CI 0.063 - 0.259), and a3b30.087 (P0.012, 95% CI 0.019 - 0.186). Conclusion · Emotional intelligence interferes in behavioral patternaffecting crisis intervention learning performance, but influence relationships are inconsistent.
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    Relationship of health promotion lifestyle with social support and self-efficacy of the elderly in urban-rural communities
    LUAN Wei, ZHU Zhu, ZHU Dong-ping, GE Wen-jing, ZHU Qun-mei, JIANG Yan-qing, LI Jin, JIANG An-li
       2020, 40 (1): 107-.   DOI: 10.3969/j.issn.1674-8115.2020.01.017
    Abstract690)      PDF(pc) (6647KB)(360)       Save
    Objective · To investigate the health promoting lifestyle of the elderly in one urban-rural community in Shanghai, and explore its relationship with social support and self-efficacy. Methods · Using cluster sampling method, 984 senior citizens in a community of Pujiang Town, Minhang District, Shanghai were investigated with general situation, Social Support Rating Scale (SSRS), General Self-Efficacy Scale (GSES) and Health Promotion Lifestyle Profile Ⅱ (HPLPⅡ ). Results · The total score of social support was 40.51±5.92. The total score of general self-efficacy was 2.35±0.58. The total score of health-promoting lifestyle was 120.44±22.75. The total score of SSRS, subjective support and utilization of support, and the total score of GSES were positively correlated with the scores of all dimensions and total scores of HPLPⅡ(PConclusion · The level of health promoting lifestyle of the elderly in the urban-rural community needs to be improved. Among them, the score of nutrition dimension is the highest, and the score of exercise dimension is the lowest. The health promotion behavior and health level of the elderly in the community can be improvedimproving social support and self-efficacy.
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    Research progress on suicide of schizophrenia patients
    ZHANG Jian-biao, Lü Qin-yu, XU Jian, YI Zheng-hui
       2020, 40 (1): 112-.   DOI: 10.3969/j.issn.1674-8115.2020.01.018
    Abstract833)      PDF(pc) (10084KB)(328)       Save
    Suicide refers to the act of voluntarily and actively ending ones life. Schizophrenia is a serious mental illness with a high risk of suicide, which has a great impact on individuals and families. Suicide is the most important caof premature death in schizophrenia patients. The paper reviews recent advances in suicide-related risk factors, suicidal characteristics of patients with different characteristics, and suicide intervention in schizophrenia patients.
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    Research progress of the mechanism and treatment of macrophage in spinal cord injury repair
    LIN Jun-qing, ZHENG Xian-you, BAO Bing-bo, LI Xing-wei, GAO Tao, HUANG Teng-li
       2020, 40 (1): 118-.   DOI: 10.3969/j.issn.1674-8115.2020.01.019
    Abstract1295)      PDF(pc) (8721KB)(517)       Save
    Self-repair has been initiated after spinal cord injury (SCI). Unlike wound healing, the self-regeneration of spinal cord is incomplete and insufficient. Many studies show that there are a lot of cells and factors active in self-repair of SCI. Inflammatory is a hard problem for us to face in SCI. Over-inflammatory will casecondary damage to the spinal cord and the capacity of self-repair. In the inflammatory environment, Many kinds of research evidence prove that macrophage pays a vital role in the over inflammatory with damaging the self-repair of SCI. Thus, targeting macrophage in spinal cord injury will hopefully become a method in SCI repair. This review clarifies the function and mechanism of macrophage in SCI repair and the new targeting macrophage methods in recovering the self-repair capacity of SCI.
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    Recent advances in role of monocyte-macrophage in anti-neutrophil cytoplasmic antibody associated vasculitis
    AN Xiao-ning, CHEN Yong-xi
       2020, 40 (1): 123-.   DOI: 10.3969/j.issn.1674-8115.2020.01.020
    Abstract638)      PDF(pc) (8819KB)(366)       Save
    Anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV) constitues a group of autoimmune diseases with poor prognosis. Inflammation and fibrinoid necrosis of the small vessels are the pathological features of the disease, and many organs and systems can be involved. Monocyte-macrophages are important to innate immune system. Monocyte-macrophage can respond rapidly to inflammation and participate in the progression of AAV. Recently, the role of monocyte-macrophage in AAV has been studied with great detail. This article reviews recent research progress of monocyte-macrophage in AAV so as to further understand the disease.
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    Clinical application of PD-1/PD-L1 to prognosis prediction and treatment of triple-negative breast cancer
    ZHANG Feng-chun, ZHANG Shuo-yuan, CHEN Tian-en, XU Ying-chun
       2020, 40 (1): 128-.   DOI: 10.3969/j.issn.1674-8115.2020.01.021
    Abstract997)      PDF(pc) (10336KB)(473)       Save
    Programmed death-1 (PD-1) receptor and programmed death-ligand 1 (PD-L1) as a pair of T cell immune response co-stimulatory molecules play a negative role in adoptive immunityinhibiting T lymphocyte function. Blocking the PD-1/PD-L1 signaling pathway has been a hot spot of research and treatment of cancer. Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer, and there is no approved targeted therapy. Immunotherapy representedPD-1/PD-L1 blockers, is getting widely studied due to the medical potential of therapy for TNBC. Thereinto, the clinical researches of the monoclonal antibodies against PD-1 or PD-L1 have brought a promising future for the treatment of metastatic TNBC. Currently, there are several trials with anti-PD-1 or anti-PD-L1 monoclonal antibodies and their combination with other therapies in salvage, neoadjuvant, and adjuvant settings on going. In this article, the authors review the current studies about potential value of PD-1/PD-L1 in prognosis, prediction, and treatment in TNBC, intending to cast insight on future basic and clinical studies.
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    Recent advances in using of stem cell-derived extracellular vesicles in treating central nervous system diseases
    HE Zheng-hui, WANG Yang, DENG Zhi-feng
       2020, 40 (1): 134-.   DOI: 10.3969/j.issn.1674-8115.2020.01.022
    Abstract870)      PDF(pc) (9142KB)(503)       Save
    Stem cell-derived extracellular vesicles can regulate functions of targeting cells in lesions and have fewer risks in treating diseases. In central nervous injury lesions, stem cell-derived extracellular vesicles can promote neurogenesis and angiogenesis and regulate nervous inflammation, which promotes the recovery of the nerve injury. Stem cell-derived extracellular vesicles can ease Aβ protein-related neuron damage in Alzheimers disease. Stem cell-derived extracellular vesicles also can deliver microRNA and chemotherapeutic agents to neuroglioma cells. This review introduces recent advances in using stem cell-derived extracellular vesicles in treating central nervous system diseases.
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    Clinical outcomes of modified lateral advanced flap combined with tunnel technique for the treatment of maxillary isolated soft tissue defect
    FAN Yan-dan, GONG Yin, DONG Jia-chen, SHU Rong
       2020, 40 (1): 139-.   DOI: 10.3969/j.issn.1674-8115.2020.01.023
    Abstract809)      PDF(pc) (6676KB)(553)       Save
    Objective · To observe the clinical outcome of the modified laterally advanced flap combined with tunnel technique for the treatment of maxillary isolated soft tissue defects. Methods · Three of maxillary isolated soft tissue defects were selected, and the modified lateral advanced flap combined with tunnel technique and subepithelial connective tissue graft was performed to repair the soft tissue defects. Pocket depth (PD), clinical attachment loss (CAL), recession height (RD), recession weight (RW), gingiva thickness (GT), and keratinized tissue width (KTW) were measured at baseline and one-year follow-up after treatment and the root coverage rate (RC%) was calculated. Results · Totally three patients were treated, two Miller Ⅲ and one Miller Ⅱ. The mean RC% at one-year follow-up was (78.89±18.36)%. The mean CALs at baseline and follow-up were (6.00±1.00) mm and (1.83±1.61) mm. The mean RHs at baseline and follow-up were (5.33±0.58) mm and (1.17±1.04) mm. The mean RWs at baseline and follow-up were (6.00±1.32) mm and (4.50±3.91) mm. The mean GTs at baseline and follow-up were (0.83±0.29) mm and (1.83±0.76) mm. The mean KTWs at baseline and follow-up were (0.83±0.76) mm and (5.50±0.50) mm. Conclusion · In the treatment of maxillary isolated soft tissue defect, the modified lateral advanced flap combined with tunnel technique has broad application and valid clinical outcome.
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    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1-1.  
    Abstract561)      PDF(pc) (3210KB)(369)       Save
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    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 2-2.  
    Abstract460)      PDF(pc) (19245KB)(128)       Save
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    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 3-3.  
    Abstract383)      PDF(pc) (1891KB)(275)       Save
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    Some considerations about the development of modern orthognathic surgery
    QIU Wei-liu
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1315-1317.   DOI: 10.3969/j.issn.1674-8115.2020.10.001
    Abstract724)      PDF(pc) (4123KB)(558)       Save
    This article discusses the development and concept of modern orthognathic surgery from four aspects: modern orthognathology must have the participation of orthodontist; modern orthognathic surgery has a wide range of disciplines and involves many disciplines; orthognathic surgery, craniofacial surgery, and craniomaxillofacial surgery have their own characteristics; whether orthognathic and craniofacial surgery can be integrated together.
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    Concept formation of non-expansion technology in stem cell therapy and its application prospect
    DAI Ke-rong
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1318-1320.   DOI: 10.3969/j.issn.1674-8115.2020.10.002
    Abstract529)      PDF(pc) (4998KB)(259)       Save
    Stem cell research starting in the 20th century has opened a magical door for clinical cell therapy. However, stem cell therapy has been only promoted in blood diseases up to now, while in other clinical fields, it still remains in the stage of slow development. One of the important restrictions is that the clinical application of stem cells usually need in vitro expansion, resulting in consequences such as the longtime-culture process, tendency to contaminate, high cost, and physical and chemical impact factors, and these could in a certain extent increase the risk both in pre- and post-treatment. As for the use of embryonic stem cells, it could be questioned by ethics for the harvest, the use of animal serum, and the non-diploid unstable cells that may appear after stem cell expansion in vitro. Then, some non-expansion technologies, such as the centrifugation technology, immunomagnetic beads technology, and stem cell screen-enrich-combine(-biomaterials) circulating system, have gradually been paid more attentions, and formed the new concept that are continuously developed. By these techniques, efficient therapeutic doses of stem cells could be obtained without culture in vitro, and the stem cells could be transplanted directly. These technologies have gradually shown good prospects in clinical application and promotion.
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    Oral and Maxillofacial Phenome Project: a new starting point for the sustainable development of stomatology
    ZHANG Zhi-yuan
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1321-1323.   DOI: 10.3969/j.issn.1674-8115.2020.10.003
    Abstract575)      PDF(pc) (3924KB)(189)       Save
    Human Phenome Project is a major theoretical innovation and breakthrough in the field of scientific research, and a new national science and technology strategy. Oral and Maxillofacial Phenome Project also provides a scientific theoretical basis and a new starting point for the sustainable development of stomatology. In this paper, the leading role of the Oral and Maxillofacial Phenome Project in stomatology is described, and the bridge effect between the clinical research and translational medicine on stomatology is illustrated with examples.
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    Auditory brainstem implantation in young children with congenital deafness: a case report
    JIA Huan1, 2*, CHEN Ying1, 2*, ZHANG Zhi-hua1, 2, LI Jing-jie3, LI Yun1, 2, CHEN Jian-qing1, 2, LI Bei1, 2, TAN Hao-yue1, 2, WANG Zhao-yan1, 2, WU Hao1, 2
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1324-1329.   DOI: 10.3969/j.issn.1674-8115.2020.10.004
    Abstract797)      PDF(pc) (7980KB)(267)       Save
    Objective · To investigate the safety and effectiveness of the first series of auditory brainstem implantation (ABI) in young children with congenital deafness in China mainland. Methods · The consecutive pediatric cases with congenital deafness and major malformations of inner ear and/or auditory nerve undergoing ABI surgery in the Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, from January to April in 2019 were retrospectively studied. Their audiological data [aided pure tone average (PTA), scores of Infant-Toddler Meaningful Auditory Integration Scale (IT-MAIS), Categories of Auditory Performance (CAP), Meaningful Use of Speech Scale (MUSS), and Speech Intelligibility Rating (SIR)], radiological data (CT and MRI), and operation and medical records were collected to evaluate the operation safety and hearing rehabilitation outcomes within 1 year after surgery. Results · Four children were included in the study with the age of (26.3±7.8) months at the time of surgery. These patients presented bilaterally profound sensorineural hearing loss. One case had bilateral common cavity malformations with cochlear nerves aplasia, and three had bilateral Michel malformations with cochlear nerves aplasia. All 4 patients were successfully implanted in right side; the pure operation time was (354.4±31.7) min with 5–15 mL hemorrhage. At the first mapping, the average number of active electrodes was 8.5±1.0. Twelve months later, the number of active electrodes was 10.0±1.6, IT-MAIS score was (31.5±1.7) score, CAP was (4.3±0.8) score, MUSS was (14.5±3.7) score, SIR was 2.0 score, and aided PTA was (38.8±5.9) dBHL. Conclusion · ABI can be safely operated in young children with bilateral profound hearing loss, and can effectively restore the hearing and promote speech development.
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    Clinical research of CO2 laser combined with photodynamic therapy in the treatment of oral leukoplakia
    YAO Yi-lin, WU Lan, TANG Guo-yao
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1330-1333.   DOI: 10.3969/j.issn.1674-8115.2020.10.005
    Abstract926)      PDF(pc) (5659KB)(360)       Save
    Objective · To explore the clinical efficacy of CO2 laser combined with photodynamic therapy (PDT) in the treatment of oral leukoplakia (OL). Methods · A total of 21 OL patients in Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from June 2018 to June 2019 were recruited. They were randomly divided into experimental group (11 cases, treated with CO2 laser combined with PDT) and control group (10 cases, treated with CO2 laser). The adverse effects during the 1st week, the clinical efficacy at the 1st month and the recurrence at the 3rd and 6th months after treatment were recorded. The differences of the above indexes between the two groups were statistically analyzed. Results · At the 1st month after treatment, the significant efficiency of the experimental group and the control group was 100% and 80%, respectively, while the total treatment efficiency were 100% and 90%, respectively. There was no significant difference between the two groups. During the 1st week post-treatment, the patients experienced varying degrees of pain, edema and (or) erosion, and there was no significant difference between the two groups. At the 3rd month after treatment, the recurrence rate of the experimental group and the control group was 0 and 10%, respectively, with no statistical difference; at the 6th month after the treatment, the recurrence rate of the experimental group was significantly lower than that of the control group (P=0.038). Conclusion · Both CO2 laser combined with PDT and single CO2 laser have significant curative effect and no serious adverse effect on the treatment of OL, and the combination therapy can significantly reduce the rate of recurrence.
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    Expression of long noncoding RNA COL11A1-208 in oral squamous cell carcinoma and its clinical significance
    JIANG Ying-ying1, 2, QIN Xing1, ZHANG Jian-jun1, CHEN Wan-tao1
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1334-1339.   DOI: 10.3969/j.issn.1674-8115.2020.10.006
    Abstract641)      PDF(pc) (8411KB)(240)       Save
    Objective · To investigate the expression of long noncoding RNA (lncRNA) COL11A1-208 in oral squamous cell carcinoma (OSCC), and evaluate its potential value in prognosis prediction and diagnosis of OSCC. Methods · The differentially expressed lncRNAs from six pairs of OSCC tissues and the para-carcinoma tissues were detected by lncRNA microarray. Quantitative real-time PCR (qPCR) was used to detect the expression of COL11A1-208 in seventy-four pairs of OSCC tissues and para-carcinoma tissues. The difference of COL11A1-208 expression among the patients with different clinical features were analyzed. The potential effectiveness of COL11A1-208 level in the diagnosis of OSCC was evaluated by the receiver operating characteristic (ROC) curve. Kaplan-Meier survival analysis was used to analyze the effects of COL11A1-208 on the 3-year overall survival rate of OSCC patients. Results · Compared with the para-carcinoma tissues, COL11A1-208 was highly expressed in OSCC tissues (P=0.000), which was consistent with the results of lncRNA microarray. The expression of COL11A1-208 in the patients with advanced TNM stage (Ⅲ - Ⅳ) was significantly higher than that with TNM stage Ⅰ - Ⅱ (P=0.001). The area under ROC curve of COL11A1-208 as a biomarker for the diagnosis of OSCC was 0.702 (95%CI 0.617 -0.788, P=0.000), and the sensitivity and specificity were 87.8% and 54.1%, respectively. The 3-year overall survival rate of the patients with low expression of COL11A1-208 was significantly longer than that of the patients with high expression (P=0.002). Conclusion · The expression of COL11A1-208 is up-regulated in OSCC tissues, which is even higher in the patients with advanced TNM stage. COL11A1-208 might have potential value in prognostic prediction and diagnosis of OSCC.
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    Experimental study of superior mesenteric artery remodeling in cirrhotic rats with portal hypertension
    ZHENG Lei, LIN Jia-yun, ZHANG Chi-hao, ZHAO Zhi-feng, LI Hong-jie, QI Xiao-liang, HUO Hai-zhong#, LUO Meng#
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1340-1346.   DOI: 10.3969/j.issn.1674-8115.2020.10.007
    Abstract720)      PDF(pc) (8381KB)(258)       Save
    Objective · To investigate the remodeling of superior mesenteric artery (SMA) in cirrhotic portal hypertension (PHT) rats. Methods · The rats were divided into normal group (n=8), PHT group with CCl4 inhalation for 8 weeks (n=8) and PHT group with CCl4 inhalation for 12 weeks (n=10). After the model was established, hemodynamic parameters of all the rats were measured. The liver tissues of the three groups were prepared for paraffin section, and then stained with hematoxylin and eosin staining (H-E staining), Masson staining and Sirius Red staining, respectively. After frozen sections of SMA tissues were prepared, H-E staining was performed to quantitatively analyze the lumen diameter, wall thickness and area of SMA. Caldesmon expression was detected by immunohistochemistry. The expression of elastin, cleaved caspase-3 protein (apoptotic signal) and Ki-67 protein (proliferation signal) were detected by immunofluorescence, and caldesmon and elastin of SMA in liquid nitrogen were detected by Western blotting. Results · Compared with the normal control group, portal pressure increased and mean arterial pressure decreased in PHT group with CCl4 inhalation for 8 and 12 weeks (all P<0.05), which meant the PHT model was successfully established. Compared with the normal group, the thickness and area of SMA vessel wall and the expression of caldesmon and elastin in SMA were decreased, and the apoptosis signal of SMA smooth muscle cells was increased in PHT group with CCl4 inhalation for 12 weeks (all P<0.05). Conclusion · The vascular remodeling of SMA in PHT with cirrhosis is thinning, and the expression of related contractile structural proteins such as caldesmon and elastin decrease sharply, while apoptosis signal of smooth muscle layer increases and related damage is serious.
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    Effect and mechanism of ginsenoside Re on intervertebral disc degeneration
    LIANG Zhi-hao, CHEN Zhi-qian, CHEN Chen, ZHOU Yi-fan, YANG Xiao, ZHAO Jie
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1347-1353.   DOI: 10.3969/j.issn.1674-8115.2020.10.008
    Abstract643)      PDF(pc) (9163KB)(237)       Save
    Objective · To clarify the therapeutic effect of ginsenoside Re on intervertebral disc degeneration (IDD) and the potential underlying mechanism. Methods · 10 ng/mL tumor necrosis factor-α (TNF-α) was used to stimulate nucleus pulposus (NP) cells. The expression of inflammation and cartilage-related genes, including matrix metalloproteinase 3 (Mmp 3), a disintegrin and metalloproteinase with thrombospondin 5 (Adamts 5), aggrecan and collagen typeⅡa1 (Col2a1), were measured and compared by real-time quantitative polymerase chain reaction with ginsenoside Re concentration varying from 12.5-50 μmol/L. After stimulation of NP cells with 10 ng/mL TNF-α, the amounts of nuclear factor-κB (NF-κB) pathway-related proteins were measured by using Western blotting with or without 50 μmol/L ginsenoside Re. Twelve 8-week-old rats were used to make coccyx degeneration models (coccygeal vertebrae 6/7 as sham, coccygeal vertebrae 7/8 as puncture), intraperitoneal injection of 50 μmol/L ginsenoside Re as treatment group (n=6) and phosphate buffered saline solution (PBS) as control group (n=6). One month later, the heights of the intervertebral discs were detected by X-ray. The rats' intervertebral disc NP tissues were observed by safranin O-fast green staining and hematoxylin-eosin staining, and histological grade was evaluated. By immunofluorescence, the expressions of TNF-α, MMP 3, aggrecan and COL2A1 were observed. Results · The expressions of inflammatory factors in the NP cells including Mmp 3 and Adamts 5 decreased and the expressions of aggrecan and Col2a1 increased under the treatment of 50 μmol/L ginsenoside Re (all P=0.000). Western blotting showed that compared with the NP cells only stimulated by TNF-α, the amounts of protein p-p65 and p-IκBα decreased significantly under the treatment of ginsenoside Re (both P=0.000). X-ray showed that compared with the puncture segment of PBS injection group, the puncture segment of ginsenoside Re injection group had better disc height indices (P=0.004) and degeneration grades (P=0.000). Immunofluorescence showed ginsenoside Re injection group expressed more aggrecan and COL2A1 (P=0.000), and less TNF-α and MMP 3 (P=0.000). Conclusion · GinsenosideRe can reduce the expressions of inflammatory factors by inhibiting NF-κB pathway in NP cells as well as maintain the disc heights and effectively alleviate the IDD level of rats.
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    Analysis on characteristics of diabetic foot ulceration-related genes and immune cells
    ZHOU Han, YANG Xiao-sheng, LIAO Chen-long, ZHANG Wen-chuan
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1354-1359.   DOI: 10.3969/j.issn.1674-8115.2020.10.009
    Abstract708)      PDF(pc) (9953KB)(166)       Save
    Objective · To explore the characteristics of diabetic foot ulceration (DFU)-related genes expression and immune cells. Methods · Gene expression data set GSE80178 was downloaded from the GEO database. Six DFU samples and three diabetic foot skin (DFS) samples were used to identify the differentially expressed genes (DEGs). The biological processes and cellular components of the DEGs were annotated by using Gene Ontology (GO). The relative proportions of immune cells among DFU samples, DFS samples and non-diabetic foot skin (nDFS) samples were calculated and compared by the deconvolution method. Results · A total of 296 DEGs were obtained, including 80 genes up-regulated and 216 genes down-regulated. GO analysis found that the DEGs were enriched in the cellular components such as exosomes and extracellular space, and in the biological processes such as keratinocyte differentiation, keratinization, and neutrophil chemotaxis. The comparison between DFS and nDFS showed no difference in immune cell proportions, while compared with DFS, the proportions of activated mast cells, resting natural killer cells, CD8+ T cells and monocytes increased in the DFU samples. Conclusion · In the pathogenesis of DFU, there may be active keratinization and immunoregulation in the lesion, and the infiltration of activated mast cells, resting natural killer cells, CD8+ T cells and monocytes may increase in the lesion. Hyperglycemia itself may have little effect on the relative number of immune cells in local skin.
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    Effects of periodontitis on the subsets of helper T cells in peripheral blood of rat models
    YUAN Yun, ZHANG Hong-ming, HUANG Hui
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1360-1364.   DOI: 10.3969/j.issn.1674-8115.2020.10.010
    Abstract617)      PDF(pc) (6686KB)(261)       Save
    Objective · To investigate the effects of periodontitis on the subsets of helper T (Th) cells in peripheral blood of rat models, so as to provide basis for the immunological study of periodontitis. Methods · Rat models of experimental periodontitis were induced by gingiva separation and silk tread ligation. They were divided into periodontitis group (n=5) and normal group (control group, n=5). Periodontal probing was performed two weeks after operation. Alveolar bone resorption was measured by micro-computed tomography (micro-CT). Peripheral blood was taken for flow cytometry to detect the proportions of Th1, Th2, Th17 and T-regulatory (Treg) cells. Difference of cell proportion between the two groups was performed with independent sample t-test. Results · The gingival tissue was swollen and easy to bleed, and alveolar bone resorption was obvious in the periodontitis group. Flow cytometry showed that the proportions of Th1 (P=0.002) and Th17 (P=0.028) cells in peripheral blood of the periodontitis group significantly increased, the proportion of Treg cells significantly decreased (P=0.002), and the proportion of Th2 cells did not change obviously. Conclusion · Periodontitis promotes the differentiation of pro-inflammatory Th cell subsets, while inhibiting the differentiation of inflammation-regulating Th cell subset in peripheral blood of rat models.
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    Comparative study on the regeneration capacity of tissue in root canals between concentrated growth factor and blood clot
    ZHANG Shi-yun, LAI Guang-yun, WANG Jun
    JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE)    2020, 40 (10): 1365-1370.   DOI: 10.3969/j.issn.1674-8115.2020.10.011
    Abstract622)      PDF(pc) (8611KB)(225)       Save
    Objective · To compare the difference of the regeneration capacity of tissue in root canals between concentrated growth factor (CGF) and blood clot by animal study. Methods · CGF was prepared from the upper arm venous blood of healthy volunteers. CGF was implanted into the root segments of isolated teeth, which is the experimental group, and the whole blood was injected into the root segments to form blood clots, which is the control group, with 18 samples in each group. The samples of the control group and experimental group were injected subcutaneously on both sides of the back of nude mice, respectively, and were taken out 4 and 6 weeks after transplantation. Hematoxylin and eosin staining (H-E staining) was used to observe the structure of newly formed tissue in root canals and calculate its area proportion. Immunohistochemical analysis, immunofluorescence analysis and modified Masson staining were used to detect the components of the newly formed tissue, and the area ratio and microvessel density of each component were calculated. Results · The structure of the newly formed tissue induced by the two groups was similar, and the main components were collagen fibers, blood vessels, nerve fibers and adipose tissue. After 4 and 6 weeks of implantation, compared with the control group, the proportion of the newly formed tissue area and microvessel density in the experimental group were higher (all P<0.05). After 6 weeks of implantation, compared with the control group, the proportion of new nerve fibers in the experimental group was increased (P=0.047). Conclusion · CGF and blood clot-induced endodontic tissue regeneration ability is similar, which may imply that CGF can be an alternative to blood clot in the regenerative endodontic treatment.
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