微RNA-30b-5p通过靶向Atg5抑制多囊卵巢综合征大鼠卵巢颗粒细胞自噬
王雪敏, 王亚楠, 牛爱琴, 叶英, 李飞

MicroRNA-30b-5p inhibits autophagy in ovarian granulosa cells in polycystic ovary syndrome rats by targeting Atg5
WANG Xuemin, WANG Yanan, NIU Aiqin, YE Ying, LI Fei
图3 各组大鼠卵巢GCmiR-30b-5pAtg5水平比较
Note:A. Relative expression of miR-30b-5p. B. Relative expression of Atg5 mRNA. C. Relative expression of Atg5 protein. D. Western blotting to detect the expression of Atg5 protein in ovarian GC of rats in each group. a—blank group; b—control group; c—miR-NC group; d—miR-30b-5p overexpression group; e—miR-30b-5p overexpression+pcDNA3.1-NC group; f—miR-30b-5p overexpression+pcDNA3.1-Atg5 group. P=0.000, compared with the blank group; P=0.000, compared with the control group; P=0.000, compared with the miR-30b-5p overexpression group.
Fig 3 Comparison of miR-30b-5p and Atg5 levels in ovarian GC of rats in each group