微RNA-30b-5p通过靶向Atg5抑制多囊卵巢综合征大鼠卵巢颗粒细胞自噬

王雪敏, 王亚楠, 牛爱琴, 叶英, 李飞

MicroRNA-30b-5p inhibits autophagy in ovarian granulosa cells in polycystic ovary syndrome rats by targeting Atg5

WANG Xuemin, WANG Yanan, NIU Aiqin, YE Ying, LI Fei

图7 各组大鼠卵巢GC中p62、Beclin-1和LC3蛋白表达 Note：A. Relative expression of p62 protein. B. Relative expression of Beclin-1 protein. C. Relative expression of LC3Ⅱ/LC3Ⅰ. D. Western blotting to detect protein expression in ovarian GC of rats in each group. a—blank group; b—control group; c—miR-NC group; d—miR-30b-5p overexpression group; e—miR-30b-5p overexpression+pcDNA3.1-NC group; f—miR-30b-5p overexpression+pcDNA3.1-Atg5 group. ①P=0.000, compared with the blank group; ②P=0.000, compared with the control group; ③P=0.000, compared with the miR-30b-5p overexpression group.

Fig 7 Expression of p62, Beclin-1 and LC3 proteins in ovarian GC of rats in each group