基于水凝胶微球建立胰腺癌原代细胞的3D培养模型

马芳芳, 秦洁洁, 任灵杰, 唐笑梅, 刘佳, 施敏敏, 蒋玲曦

Establishment of a 3D culture model in vitro of pancreatic cancer primary cells using hydrogel microspheres

MA Fangfang, QIN Jiejie, REN Lingjie, TANG Xiaomei, LIU Jia, SHI Minmin, JIANG Lingxi

图1 GelMA水凝胶微球的表征 Note：A. Bright field imaging of hydrogel microspheres under a fluorescence inverted microscope (×100). The microspheres were highly dispersed and uniform in size. B. Particle size distribution of GelMA hydrogel microspheres, with an average particle size of 200 μm. C. There was no significant difference in cell proliferation between the two groups by CCK-8 assay. The ctl was 293T cells cultured in high glucose medium, and the mic group was 293T cells cultured with GelMA porous hydrogel microsphere extract.

Fig 1 Characterization of GelMA hydrogel microspheres